5.1 Results from this test method suggest the degree of aerobic, aquatic biodegradation of a lubricant or lubricant component. The rate and extent of oxygen consumption is measured upon exposure of the test material to an inoculum within the confines of a controlled laboratory setting. Test materials which achieve a high degree of biodegradation in this test may be assumed to easily biodegrade in many aerobic aquatic environments.5.2 Because of the stringency of this test method, low results do not necessarily mean that the test material is not biodegradable under environmental conditions, but indicate that further testing is necessary to establish biodegradability.5.3 If the pH value at the end of the test is outside the range from 6 to 8 and if the percentage degradation of the test material is less than 50 %, it is advisable to repeat the test with a lower concentration of the test material or a higher concentration of the buffer solution, or both.5.4 A reference or control material known to biodegrade under the conditions of this test method is necessary in order to verify the activity of the inoculum. The test must be regarded as invalid and shall be repeated using a fresh inoculum if the reference material does not demonstrate biodegradation to the extent of >60 % of the ThO2 within 28 days.5.5 Information on the toxicity of the test material to the inoculum may be useful in the interpretation of low biodegradation results. Toxicity of the test material to the inoculum may be evaluated by testing the test material in combination with the reference material in inhibition control systems. If an inhibition control is included, the test material is assumed to be inhibiting if the degradation percentage of the reference material is lower than 40 % (ISO 8192). In this case, it is advisable to repeat the test with lower concentrations of the test material.5.6 Total oxygen utilization in the blank at the end of the test exceeding 60 mg O2/L invalidates the test.5.7 The water solubility or dispersibility of the lubricant or component may influence the results obtained and hence comparison of test results may be limited to lubricants or components with similar solubilities.5.8 The behaviors of complex mixtures are not always consistent with the individual properties of the components. Test results for individual lubricant components may be suggestive of whether a mixture containing these components (that is, fully formulated lubricants) is biodegradable, but such information should be used judiciously.1.1 This test method covers a procedure for determining the degree of biodegradability of lubricants or their components in an aerobic aqueous medium on exposure to an inoculum under controlled laboratory conditions. This test method is an ultimate biodegradation test that measures oxygen demand in a closed respirometer.1.2 This test method is suitable for evaluating the biodegradation of volatile as well as nonvolatile lubricants or lubricant components.1.3 This test method is applicable to lubricants and lubricant components which are not toxic and not inhibitory to the test microorganisms at the test concentration.1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazards are given in Section 10.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

Measurement of bacterial densities is generally the first step in establishing a relationship between bacteria and other biochemical processes. It is known that the classical plate count procedure underestimates bacterial densities while the epifluorescence direct microscopic procedure more accurately depicts the total numbers of nonviable or dormant and viable cells in a water sample. The acridine-orange INT-formazan reduction technique provides information on the total concentrations of bacteria as well as that proportion which are actively respiring and thus involved in degradative processes.The acridine-orange INT-formazan reduction technique is both quantitative and precise.This procedure is ideal for enumerating both pelagic and epibenthic bacteria in all fresh water and marine environments.The process can be employed in survey studies to characterize the bacteriological densities and activities of environmental waters.1.1 This test method covers the detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. This test method is applicable to environmental waters and potable waters.1.2 Certain types of debris and other microorganisms may fluoresce in acridine-orange stained smears.1.3 The procedure described requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.1.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.1.5 Approximately 104 cells/mL are required for detection by this test method.1.6 Minimal cell size which allows the detection of formazan deposits is represented by bacteria of 0.4 μm.21.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.8 This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

定价： 0元 / 折扣价： 0 元

5.1 Bacterial populations, as part of the microbial community in aquatic systems are actively involved in nutrient cycling. The significance of these populations is often difficult to ascertain because of the presence of many physiological types. However, measurement of bacterial densities is usually the first step in trying to establish any relationship that might exist between bacteria and other biochemical processes.45.2 Acridine-orange epifluorescence direct-counting procedure cannot differentiate between viable and nonviable cells.5.3 This procedure cannot be used to convert directly the numbers to total carbon biomass because of the natural variations in bacterial cell size.5.4 The acridine-orange epifluorescence direct-microscopic count is both quantitative and precise.5.5 This procedure is ideal for enumerating both pelagic and epibenthic bacteria in all fresh water and marine environments.55.6 The process can be employed in survey activities to characterize the bacteriological densities of environmental waters.5.7 The procedure can also be used to estimate bacterial densities in cooling tower waters, process waters, and waters associated with oil drilling wells.1.1 This test method describes a procedure for detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. It is applicable to environmental waters.1.2 Certain types of debris and other microorganisms may fluoresce in acridine orange-stained smears.1.3 The test method requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.21.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.1.5 Approximately 104 cells/mL are required for detection by this test method.21.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.7 This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

定价： 0元 / 折扣价： 0 元

4.1 Absorbed doses of or below 1 kGy can inactivate some parasites, such as the broad fish tapeworm (Dibothrocephalus latus) (2).4.2 Absorbed doses below 10 kGy can reduce or eliminate vegetative cells of pathogenic sporeforming and non-sporeforming microorganisms, such as Clostridium spp., Vibrio spp., Salmonellae, Listeria monocytogenes, or Staphylococcus aureus, that may be present in fresh or frozen product.4.2.1 Absorbed doses below 10 kGy can reduce the numbers of some spores, but are not adequate to reduce the potential health risk from microbial spores or toxins (3).4.3 Absorbed doses below 10 kGy can reduce or eliminate the vegetative cells of sporeforming and non-sporeforming microorganisms, such as Bacillus or Pseudomonas species, that cause spoilage of fresh product, thus extending refrigerated shelf life in many cases (4).1.1 This guide outlines procedures and operations for the irradiation of raw, untreated, fresh (chilled), or frozen finfish and aquatic invertebrates, while ensuring that the irradiated product is safe and wholesome.1.1.1 Aquatic invertebrates include mollusks, crustacea, echinoderms, etc.1.1.1.1 Mollusks include bivalve shellfish, such as clams, mussels, and oysters; snails; and cephalopods, such as squid and octopus.1.1.1.2 Crustacea include shellfish such as shrimp, lobster, crabs, prawns and crayfish.1.1.1.3 Echinoderms include sea urchins and sea cucumbers.1.2 This guide covers absorbed doses used to reduce the microbial and parasite populations in aquatic invertebrates and finfish. Such doses typically are below 10 kGy (1).21.2.1 This guide covers gamma, electron beam, and X-radiation treatment.1.3 The use of reduced-oxygen packaging (vacuum or modified atmosphere, and including products packed in oil) with irradiated, raw product is not covered by this guide. The anaerobic environment created by reduced-oxygen packaging provides the potential for outgrowth of, and toxin production from, Clostridium botulinum spores.1.4 This guide does not cover the irradiation of smoked or dried fish to reduce microbial load or to control insect infestation.1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.6 This document is one of a set of standards that provides recommendations for properly implementing and utilizing radiation processing. It is intended to be read in conjunction with ISO/ASTM Practice 52628.1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

5.1 This practice gives techniques to use in the preparation of lubricants or lubricant components for acute or chronic aquatic toxicity tests. Most lubricants and lubricant components are difficult to evaluate in toxicity tests because they are mixtures of chemical compounds with varying and usually poor solubility in water. Lubricants or lubricant component mixtures should not be added directly to aquatic systems for toxicity testing because the details of the addition procedure will have a large effect on the results of the toxicity test. Use of the techniques described in this practice will produce well-characterized test systems that will lead to tests with meaningful and reproducible results.5.2 The toxicity of mixtures of poorly soluble components cannot be expressed in the usual terms of lethal concentration (or the similar terms of effect concentration or inhibition concentration) because the mixtures may not be completely soluble at treat levels that lead to toxic effects. The test material preparation techniques given in this practice lead to test results expressed in terms of loading rate, which is a practical and meaningful concept for expressing the toxicity of this type of material.5.3 One of the recommended methods of material preparation for lubricants or their components is the mechanical dispersion technique. This particular technique generates turbulence, and thus, it should not be used for poorly swimming organisms.1.1 This practice covers procedures to be used in the preparation of lubricants or their components for toxicity testing in aquatic systems and in the interpretation of the results of such tests.1.2 This practice is suitable for use on fully-formulated lubricants or their components that are not completely soluble at the intended test treat rates. It is also suitable for use with additives, if the additive is tested after being blended into a carrier fluid at the approximate concentration as in the intended fully formulated lubricant. The carrier fluid shall meet the above solubility criterion, be known to be minimally toxic in the toxicity test in which the material will be tested, and be known to have a chemical composition similar to the rest of the intended fully formulated lubricant.1.3 Samples prepared in accordance with this practice may be used in acute or chronic aquatic toxicity tests conducted in fresh water or salt water with fish, large invertebrates, or algae. This practice does not address preparation of samples for plant toxicity testing other than algae.1.4 Standard acute and chronic aquatic toxicity procedures are more appropriate for lubricants with compositions that are completely soluble at the intended test treat rates (1, 2, 3, 4, 5).21.5 This practice is intended for use with lubricants or lubricant components of any volatility.1.6 This practice does not address any questions regarding the effects of any lubricant or lubricant component on human health.1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

5.1 The USEPA's policy for whole-effluent monitoring stresses, an integrated approach to toxicity testing (1, 5) tests and other measures of toxicity, should be systematically employed and should be related to certain aquatic-system factors, such as the type of habitats available (benthic and water column), flow regime, and physicochemical quality of the site water and sediment. The determination of toxicity is generally accomplished with a few surrogate species for four major reasons: a regulatory agency can compare test results between sites and over time in order to help prioritize enforcement efforts, tests using these species are relatively inexpensive since the organisms can be cultured year-round under laboratory conditions, the reliability of test methods utilizing surrogate species is better established than for other species, and surrogate species are better integrated into toxicity identification evaluations than other species. For regulatory purposes, under the National Pollution Discharge Elimination System (NPDES), USEPA considers it unnecessary to conduct whole effluent toxicity tests with resident or indigenous species (6). An alternate testing procedure protocol is provided by USEPA for validating toxicity methods using species not already approved (6,7). In systems where surrogate species are not found, erroneous predictions might be obtained of environmental impact or water and sediment quality impairment based on toxicity tests using surrogate species (8).5.2 This guide is intended to assist researchers and managers in selecting appropriate resident species for site-specific toxicity assessments. This guide could be used to select a resident species for use in predicting the potential toxic effects of a substance in certain types of aquatic environments. Another use might be for selecting a number of indigenous species from the aquatic community, that when tested, might indicate potential toxic effects of the test substance or material on the ecological integrity of that community. Selection of a suitable test species is very important because species might respond quite differently to toxic compounds (9). Species suggested as test organisms by regulatory agencies might not occur in the receiving waters of interest and their sensitivity to a toxic substance might not be representative of the sensitivity exhibited by resident species. Since aquatic ecosystem structure and function is often determined by a few key species (10, 11, 12, 13), toxicological tests with these resident species might be very important.5.3 This guide can be used in the selection of representative test species for certain site-specific assessments, such as the Resident-Species Criteria Modification Procedure (1), the Recalculation Procedure (14), and ecological risk assessment studies.5.4 This guide can be used as a general framework for researchers who desire to develop or modify existing toxicity test methods for previously untested species.5.5 Researchers in countries other than the United States and Canada might obtain useful information from this guide regarding potential test species or test methods for sites of local interest.1.1 This guide along with Guide E1192 and guidance from the U.S. Environmental Protection Agency (1,2)2 covers the use of resident species in toxicity testing, particularly if site-specific information is desired. For example, in those systems where particular species are considered to be economically or aesthetically important, it might be more appropriate to utilize resident species for testing (3). For this reason, the USEPA allows development of site-specific chemical standards, using resident species, in order to reflect local conditions (1). This guide is designed to guide the selection of resident species for use as test organisms in aquatic and sediment toxicity tests. It presupposes that the user is familiar with the taxonomy of aquatic and benthic species and has some field experience.1.2 Because toxicological information is often limited for many aquatic species, it is assumed that the majority of testing applications will be acute tests. Therefore, much of the guidance presented in this guide pertaining to the species selection process is applicable when acute toxicity testing is the desired goal. However, the principles discussed in this guide pertain to chronic toxicity test applications as well, although it should be clearly understood that such testing requires substantially greater effort, time, and resources than acute testing.1.3 The procedures for selecting resident species in toxicity testing are necessarily general at this time because information is often lacking for specific taxa or groups of taxa. This guide attempts to give specific information when appropriate.1.4 This guide is not intended to be inclusive. References listed provide a starting point from which to approach the literature. This guide deals solely with aquatic toxicity test situations. Terrestrial, arboreal, or atmospheric species are not considered in this guide.1.5 This guide is arranged as follows:  Section    1  Referenced Documents 2  Terminology 3  Summary of Guide 4   5  Species Selection Process 6  Collection of Information 6.1Obtaining Resident Species for Toxicity Testing 6.2Criteria for Selection 6.3Test Performance Characterization 6.4Interferences 7  Safety Precautions 8  Documentation 9  Keywords 10   AppendixesPotential Test Species  Appendix X1  Algae  X1.1Aquatic Floating Macrophytes  X1.2Protozoa  X1.3Rotifera  X1.4Attached and Benthic Fauna  X1.5Fish  X1.6Amphibia  X1.7Examples of Resident Species  Table X1.1Taxonomic Keys—Partial Listing  Appendix X2  Flow Chart of Factors to Consider For Selecting A Resident Species  Appendix X3  1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. All safety precautions and health-related practices are the responsibility of the user. Specific safety practices are suggested in Section 8.1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 646元 / 折扣价： 550 元 加购物车

5.1 Polychaetes are an important component of the benthic community, in which they generally comprise 30 to 50 % of the macroinvertebrate population. They are preyed upon by many species of fish, birds, and larger invertebrate species. Larger polychaetes feed on small invertebrates, larval stages of invertebrates, and algae. Polychaetes are especially sensitive to inorganic toxicants and, to a lesser extent, to organic toxicants (1).4 The ecological importance of polychaetes and their wide geographical distribution, ability to be cultured in the laboratory, and sensitivity to contaminants make them appropriate acute and chronic toxicity test organisms. Their relatively short life cycle enables the investigator to measure the effect of contaminants on reproduction.5.2 An acute toxicity or chronic text is conducted to obtain information concerning the immediate effects of an exposure to a test material on a test organism under specified experimental conditions. An acute toxicity test provides data on the short-term effects, which are useful for comparisons to other species but do not provide information on delayed effects. Chronic toxicity tests provide data on long-term effects.5.3 A life-cycle toxicity test is conducted to determine the effects of the test material on survival, growth, and reproduction of the test species. Additional sublethal endpoints (for example, biochemical, physiological, and histopathological) may be used to determine the health of the species under field conditions.5.4 The results of acute, chronic, and life-cycle toxicity tests can be used to predict effects likely to occur on marine organisms under field conditions.5.5 The results of acute, chronic, or life-cycle toxicity tests might be used to compare the sensitivities of different species and the toxicities of different test materials, as well as to study the effects of various environmental factors on the results of such tests.5.6 The results of acute, chronic, or life-cycle toxicity tests might be an important consideration when assessing the hazards of materials to marine organisms (see Guide E1023) or when deriving water quality criteria for aquatic organisms (2).5.7 The results of acute, chronic, or life-cycle toxicity tests might be useful for studying the biological availability of, and structure activity relationships between, test materials.5.8 The results of acute, chronic, and life-cycle toxicity tests will depend partly on the temperature, quality of food, condition of test organisms, test procedures, and other factors.1.1 This guide covers procedures for obtaining data concerning the adverse effects of a test material added to marine and estuarine waters on certain species of polychaetes during short- or long-term continuous exposure. The polychaete species used in these tests are either field collected or from laboratory cultures and exposed to varying concentrations of a toxicant in static or static-renewal conditions. These procedures may be useful for conducting toxicity tests with other species of polychaetes, although modifications might be necessary.1.2 Modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute, chronic, or life-cycle tests with other species of polychaetes.1.3 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, and known or unknown mixtures. With appropriate modifications, these procedures can be used to conduct these tests on factors such as temperature, salinity, and dissolved oxygen. These procedures can also be used to assess the toxicity of potentially toxic discharges such as municipal wastes, sediments/soils, oil drilling fluids, produced water from oil well production, and other types of industrial wastes. An LC50 (medial lethal concentration) may be calculated from the data generated in each acute and chronic toxicity test when multiple concentrations are tested. Growth, determined by a change in measured weight, and reproduction, as the change in total number of organisms, are used to measure the effect of a toxicant on life-cycle tests; data are analyzed statistically to indicate that concentration at which a significant difference occurs between the test solutions and control(s).1.4 The results of dose-response acute or chronic toxicity tests with toxicants added experimentally to salt water should usually be reported in terms of an LC50 (mortality), or EC50 (medial effect concentration). The results of life-cycle toxicity tests with toxicants added experimentally to salt water should be reported as that concentration at which a statistically significant difference in the number of offspring or growth (determined by weight) is produced with reference to the control(s).1.5 Where appropriate, this standard has been designed to be consistent with or complementary to other methods for assessing toxicity to invertebrates described in Test Methods E1367 and E1706, and Guides E1391, E1525, E1611, and E1688.1.6 This guide is arranged as follows:  SectionReferenced Documents  2Terminology  3Summary of Guide  4  5Apparatus  6 Facilities  6.1 Construction Materials  6.2 Test Chambers  6.3 Cleaning  6.4 Acceptability  6.5Safety Precautions  7Dilution Water  8 Requirements  8.1 Source  8.2 Treatment  8.3 Characterization  8.4Test Material  9 General  9.1 Stock Solution  9.2 Test Concentrations  9.3Test Organisms 10 Species 10.1 Age 10.2 Source 10.3 Feeding 10.4 Holding 10.5 Quality 10.6Procedure 11 Experimental Design 11.1  Acute Test 11.1.1  Chronic Test 11.1.2  Life-Cycle Test 11.1.3 Test Condition Specifications 11.2  Dissolved Oxygen 11.2.1  Temperature 11.2.2  Loading 11.2.3  Salinity 11.2.4  Light 11.2.5 Beginning the Test 11.3 Feeding 11.4 Duration of Test 11.5 Biological Data 11.6 Other Measurements 11.7Hazards  Analytical Methodology 13Acceptability of Test 14Calculation of Results 15Report 16Keywords 17Appendixes:   Neanthes arenaceodentata Appendix X1 Capitella capitata Appendix X2 Ophryotrocha diadema Appendix X3 Dinophilus gyrociliatus Appendix X41.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 7.1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 843元 / 折扣价： 717 元 加购物车

5.1 Protection of a species requires the prevention of detrimental effects of chemicals on the survival, growth, reproduction, and health of that species. Behavioral toxicity provides information concerning sublethal effects of chemicals and signals the presence of toxic test substances.5.1.1 The behavioral responses of all organisms are adaptive and essential to survival. Major changes in the behavioral responses of fish, amphibians, and macroinvertebrates may result in a diminished ability to survive, grow, or reproduce and cause significant changes in the natural population (8).5.2 The results from behavioral toxicity tests may be useful for measuring injury in the assessment of damages resulting from the release of hazardous materials (9) .5.3 Behavioral toxicity test methods may be useful for long-term monitoring of effluents (10) .5.4 The results from behavioral toxicity data can be used to predict the effects of exposure on fish, amphibians, and aquatic invertebrates likely to occur in field situations as a result of exposure under similar conditions, including the avoidance of exposure by motile organisms (11).5.5 The results from behavioral toxicity tests might be an important consideration for assessing the hazard of materials to aquatic organisms. Such results might also be used when deriving water quality criteria for fish and aquatic invertebrate organisms.5.6 The results from behavioral toxicity tests can be used to compare the sensitivities of different species, relative toxicity of different chemical substances on the same organism, or effect of various environmental variables on the toxicity of a chemical substance.5.7 The results from behavioral toxicity tests can be used to predict the effects of long-term exposure.5.8 The results of behavioral toxicity tests can be useful for guiding decisions regarding the extent of remedial action needed for contaminated aquatic and terrestrial sites.5.9 The behavioral characteristics of a particular organism must be understood and defined before a response can be used as a measure of toxicity. The range of variability of any behavioral response of unexposed organisms is influenced by genetic, experiential, physiological, and environmental factors. Thus it is important to avoid selecting test organisms from populations that may vary significantly in these factors.5.10 The results of behavioral toxicity tests will depend on the behavioral response measured, testing conditions, water quality, species, genetic strain, life stage, health, and general condition of test organisms. Therefore, the behavioral response may be affected by the test environment.1.1 This guide covers some general information on the selection and application of behavioral methods useful for determining the sublethal effects of chemicals to fish, amphibians, and macroinvertebrates.1.2 Behavioral toxicity occurs when chemical or other stressful conditions, such as changes in water quality or temperature, induce a behavioral change that exceeds the normal range of variability (1).2 Behavior includes all observable, recordable, or measurable activities of a living organism and reflects genetic, neurobiological, physiological, and environmental determinants (2).1.3 Behavioral methods can be used in biomonitoring, the determination of no-observed-effect and lowest-observed-effect concentrations, and the prediction of hazardous chemical impacts on natural populations (3).1.4 Behavioral methods can be applied to fish, amphibians, and macroinvertebrates in standard laboratory toxicity tests, tests of effluents, and sediment toxicity tests.1.5 The various behavioral methods included in this guide are categorized with respect to seven interdependent, functional responses that fish, amphibians, and macroinvertebrates must perform in order to survive. These functional responses include respiration, locomotion, habitat selection, feeding, predator avoidance, competition, and reproduction (4). These responses can be documented visually or through video or acoustic imagery. Electronically recorded information can be derived through manual techniques or through the use of digital image analysis software (5, 6, 7).1.5.1 The functional responses are not necessarily mutually exclusive categories. For instance, locomotion, of some form of movement, is important to all behavioral functions.1.6 Additional behavioral methods for any category may be added when new tests are developed as well as when methods are adapted to different species or different life stages of an organism.1.7 This guide is arranged as follows:  Section Number  1Referenced Documents  2Terminology  3Summary of Guide  4  5Interferences  6Test Facility  7Water Supply  8Safety Precautions  9Test Material 10Test Organisms 11Responses Measured 12Behavioral Test Method Selection Criteria 13Experimental Design 14Acceptability of Test 15Calculation of Test Results 16Report 171.8 The values stated in SI units are to be regarded as the standard. For an explanation of units and symbols, refer to IEEE/ASTM SI 10.1.9 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 9.1.10 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

5.1 Results from this CO2 evolution test method suggest, within the confines of a controlled laboratory setting, the degree of ultimate aerobic aquatic biodegradability of a lubricant or components of a lubricant. Test materials which achieve a high degree of biodegradation in this test method may be assumed to easily biodegrade in many aerobic aquatic environments. (See also Test Method D5864.)5.2 Because of the stringency of this test method, a low yield of CO2 does not necessarily mean that the test material is not biodegradable under environmental conditions, but indicates that further testing needs to be carried out in order to establish biodegradability.5.3 Information on the toxicity of the test material to the inoculum may be useful in the interpretation of low biodegradation results.5.4 Activated sewage-sludge from a sewage treatment plant that principally treats domestic waste may be used as an aerobic inoculum. An inoculum derived from soil or natural surface waters, or any combination of the three sources, may also be used in this test method.NOTE 1: Allowance for various and multiple inoculum sources provides access to a greater diversity of biochemical competency and potentially represents more accurately the capacity for biodegradation.5.5 A reference or control material known to biodegrade under the conditions of this test method is necessary in order to verify the activity of the inoculum. The test method must be regarded as invalid and should be repeated using a fresh inoculum if the reference does not demonstrate biodegradation to the extent of >60 % of the theoretical CO2 within 28 days.5.6 The water solubility or dispersibility of the lubricant or components may influence the results obtained and hence the procedure may be limited to comparing lubricants or components with similar solubilities.5.7 The ratio of carbon incorporated into cellular material to carbon metabolized to CO2 will vary depending on the organic substrate, on the particular microorganisms carrying out the conversion, and on the environmental conditions under which the conversion takes place. In principle, this variability complicates the interpretation of the results from this test method.5.8 The behavior of complex mixtures may not always be consistent with the individual properties of the components. The biodegradability of the components may be suggestive of whether a mixture containing these components (that is, a fully formulated lubricant) is biodegradable but such information should be used judiciously.1.1 This test method covers the determination of the degree of aerobic aquatic biodegradation of fully formulated lubricants or their components on exposure to an inoculum under controlled laboratory conditions. This test method is an ultimate biodegradation test that measures carbon dioxide (CO2) evolution.1.2 This test method is intended to specifically address the difficulties associated with testing water insoluble materials and complex mixtures such as are found in many lubricants.1.3 This test method is designed to be applicable to all non-volatile lubricants or lubricant components that are not toxic and not inhibitory at the test concentration to the organisms present in the inoculum.1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazards are discussed in Section 10.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

5.1 Results from the test method suggest, within the confines of a controlled laboratory setting, the degree of aerobic aquatic biodegradation of a lubricant or components of a lubricant by measuring the evolved carbon dioxide upon exposure of the test material to an inoculum. The plateau level of CO2 evolution in this test method will suggest the degree of biodegradability of the lubricant. Test substances that achieve a high degree of biodegradation in this test may be assumed to easily biodegrade in many aerobic aquatic environments.5.2 Because of the stringency of this test, a low yield of CO2 does not necessarily mean that the test substance is not biodegradable under environmental conditions, but indicates that further testing is necessary to establish biodegradability.5.3 Information on toxicity to the inoculum of the test substance may be useful in the interpretation of low biodegradation results.5.4 Activated sewage-sludge from a sewage-treatment plant that principally treats domestic waste is considered an acceptable active aerobic inoculum available over a wide geographical area in which to test a broad range of lubricants. An inoculum derived from soil or natural surface waters, or both, or any combination of the three sources, is also appropriate for this test method.NOTE 1: Allowance for various and multiple inoculum sources provides access to a greater diversity of biochemical competency and potentially represents more accurately the capacity for biodegradation.5.5 A reference or control substance known to biodegrade is necessary in order to verify the activity of the inoculum. The test must be regarded as invalid and should be repeated using a fresh inoculum if the reference does not demonstrate a biodegradation of >60 % of the theoretical CO2 evolution within 28 days.5.6 A total CO2 evolution in the blank at the end of the test exceeding 75 mg CO2 per 3 L of medium shall be considered as invalidating the test.5.7 The water solubility or dispersibility of the lubricant or component may influence the results obtained and hence the procedure may be limited to comparing lubricants or components with similar solubilities.5.8 The ratio of carbon incorporated into cellular material to carbon released as CO2 will vary depending on the organic substrate, on the particular microorganisms carrying out the conversion, and on the environmental conditions under which the conversion takes place. In principle, this variability complicates the interpretation of the results from this test method.1.1 This test method covers the determination of the degree of aerobic aquatic biodegradation of fully formulated lubricants or their components on exposure to an inoculum under laboratory conditions.1.2 This test method is intended to specifically address the difficulties associated with testing water insoluble materials and complex mixtures such as are found in many lubricants.1.3 This test method is designed to be applicable to all lubricants that are not volatile and are not inhibitory at the test concentration to the organisms present in the inoculum.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazards are discussed in Section 10.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

This specification covers performance requirements for biodegradable hydraulic fluids with low aquatic toxicity used in industrial/mobile hydraulic applications. There are some cases where biodegradable fluids have been found to perform differently than traditional mineral oils, which makes separate performance requirements desirable.1.1 This specification covers performance requirements for biodegradable hydraulic fluids with low aquatic toxicity used in industrial/mobile hydraulic applications.1.2 In some cases, biodegradable fluids have been found to perform differently than traditional mineral oils, thus separate performance requirements are desirable.1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

5.1 Adverse effects on natural populations of aquatic organisms and their uses have demonstrated the need to assess the hazards of many new, and some presently used, materials. The process described herein will help producers, users, regulatory agencies, and others to efficiently and adequately compare alternative materials, completely assess a final candidate material, or reassess the hazard of a material already in use.5.2 Sequential assessment and feedback allow appropriate judgments concerning efficient use of resources, thereby minimizing unnecessary testing and focusing effort on the information most pertinent to each material. For different materials and situations, assessment of hazard will appropriately be based on substantially different amounts and kinds of biological, chemical, physical, and toxicological data.5.3 Assessment of the hazard of a material to aquatic organisms and their uses should never be considered complete for all time. Reassessment should be considered if the amount of production, use, or disposal increases, new uses are discovered, or new information on biological, chemical, physical, or toxicological properties becomes available. Periodic review will help assure that new circumstances and information receive prompt appropriate attention.5.4 If there is substantial transformation to another material, the hazard of both materials may need to be assessed.5.5 In many cases, consideration of adverse effects should not end with completion of the hazard assessment. Additional steps should often include risk assessment, decisions concerning acceptability of identified hazards and risks, and mitigative actions.5.6 Because this practice deals mostly with adverse effects on aquatic organisms and their uses, it is important that mitigative actions, such as improved treatment of aqueous effluents, not result in unacceptable effects on non-aquatic organisms. Thus, this standard should be used with other information in order to assess hazard to both aquatic and non-aquatic organisms.1.1 This guide describes a stepwise process for using information concerning the biological, chemical, physical, and toxicological properties of a material to identify adverse effects likely to occur to aquatic organisms and their uses as a result of release of the material to the environment. The material will usually be a specific chemical, although it might be a group of chemicals that have very similar biological, chemical, physical, and toxicological properties and are usually produced, used, and discarded together.1.2 The hazard assessment process is complex and requires decisions at a number of points; thus, the validity of a hazard assessment depends on the soundness of those decisions, as well as the accuracy of the information used. All decisions should be based on reasonable worst-case analyses so that an appropriate assessment can be completed for the least cost that is consistent with scientific validity.1.3 This guide assumes that the reader is knowledgeable in aquatic toxicology and related pertinent areas. A list of general references is provided (1).21.4 This guide does not describe or reference detailed procedures for estimating or measuring environmental concentrations, or procedures for determining the maximum concentration of test material that is acceptable in the food of predators of aquatic life. However, this guide does describe how such information should be used when assessing the hazard of a material to aquatic organisms and their uses.1.5 Because assessment of hazard to aquatic organisms and their uses is a relatively new activity within aquatic toxicology, most of the guidance provided herein is qualitative rather than quantitative. When possible, confidence limits should be calculated and taken into account.1.6 This guide provides guidance for assessing hazard but does not provide guidance on how to take into account social considerations in order to judge the acceptability of the hazard. Judgments concerning acceptability are social as well as scientific, and are outside the scope of this guide.1.7 This guide is arranged as follows:  SectionReferenced Documents 2 Descriptions of Terms Specific to This Standard 3 Summary of Guide 4  5 Four Basic Concepts 6  The Iteration 6.1 The Two Elements 6.2 The Possible Decisions 6.3 The Phased Approach 6.4Phase I—Use of Low-Cost (Existing) Information 7  Collection of Available Data 7.1 Initial Estimates of Environmental Concentrations 7.2 Initial Estimate of Toxicity to Aquatic Organisms 7.3 Initial Estimate of Bioaccumulation by Aquatic Organ-  isms  7.4 Phase I Hazard Assessment 7.5Phase II—Use of Medium-Cost Information 8  Improved Estimates of Environmental Concentrations 8.2 Acute Toxicity to Aquatic Animals 8.3 Toxicity to Algae 8.4 Expansion of Short-Term Testing 8.5 Bioaccumulation 8.6 Phase II Hazard Assessment 8.7Phase III—Use of High-Cost Information 9  Refined Estimates of Environmental Concentrations 9.2 Chronic Toxicity to Aquatic Animals 9.3 Use of Acute-Chronic Ratios 9.4 Toxicity to Aquatic Plants 9.5 Bioconcentration 9.6 Bioaccumulation from Food 9.7 Phase III Hazard Assessment 9.8Appendixes Appendix X1 Production, Use, Disposal, and Other Release Appendix X2 Biological Considerations Appendix X3 Chemical Considerations Appendix X4 Physical Considerations Appendix X5 Toxicological Considerations Appendix X6 Estimating Environmental Concentrations Appendix X7 Selection of Test Species Appendix X8 Long-Term Toxicity Tests1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 646元 / 折扣价： 550 元 加购物车

5.1 Cyanide and hydrogen cyanide are highly toxic. Regulations have been established to require the monitoring of cyanide in industrial and domestic wastes and surface waters.45.2 It is useful to determine the aquatic free cyanide to establish an index of toxicity when a wastewater is introduced into the natural environment at a given pH and temperature.5.3 This test method is applicable for natural water, saline waters, and wastewater effluent.5.4 Free cyanide measured using this test method is applicable for implementation of the International Cyanide Code Guidance in accordance with Guide D7728.1.1 This test method is used to establish the concentration of free cyanide in an aqueous wastewater, effluent and in-stream free cyanide concentrations after mixing treated water with receiving water. The test conditions of this test method are used to measure free cyanide (HCN and CN–) and cyanide bound in the metal-cyanide complexes that are easily dissociated into free cyanide ions at the pH of 6. Free cyanide is determined at pH 6 at room temperature. The aquatic free cyanide can be determined by matching the pH to the water in the receiving environment in the range of pH 6 to 8. The extent of HCN formation is less dependent on temperature than the pH; however, the temperature can be regulated if deemed necessary for aquatic free cyanide to further simulate the actual aquatic environment.1.2 The free cyanide test method is based on the same instrumentation and technology that is described in Test Method D6888, but employs milder conditions (pH 6–8 buffer versus HCl or H2SO4 in the reagent stream), and does not utilize ligand displacement reagents.1.3 The aquatic free cyanide measured by this procedure should be similar to actual levels of HCN in the original aquatic environment. This in turn may give a reliable index of toxicity to aquatic organisms.1.4 This procedure is applicable over a range of approximately 5 to 500 μg/L (parts per billion) free cyanide. Sample dilution may increase cyanide recoveries depending on the cyanide speciation; therefore, it is not recommended to dilute samples. Higher concentrations can be analyzed by increasing the range of calibration standards or with a lower injection volume. In accordance with Guide E1763 and Practice D6512 the lower scope limit was determined to be 9 μg/L for chlorinated gold leaching barren effluent water and the IQE10 % is 12 µg/L in the gold processing detoxified reverse osmosis permeate waste water sample matrix.1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.6 This test method is not recommended for samples that contain reduced sulfur compounds such as sulfides.1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in 8.6 and Section 9.1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 646元 / 折扣价： 550 元 加购物车

1.1 This practice covers information for manufacture, construction, operations and maintenance of aquatic play equipment and provides safety performance standards for various types of public aquatic play components and aquatic play composite structures.1.2 Inclusions: 1.2.1 Climbable and climb-resistant aquatic play components, composite aquatic play structures, user controls, water sprays, fountains, and slides that occur on wet decks and wading, swimming or leisure pools. The play components specified herein occur for use in aquatic play areas.1.2.2 Play equipment, fall zones, use zones for wet decks, wading pools, swimming pools and leisure pools.1.2.3 This standard is intended to apply to Aquatic Play Equipment that is located in and around re- circulated and potable water recreational facilities. Such facilities include but are not limited to amusement parks, theme parks, water parks, family entertainment centers, municipal swimming pools and municipal parks.1.2.4 Waterslides 6 ft in height or smaller.1.3 Exclusions: 1.3.1 Playground equipment that does not have an entry or an exit onto or into a wet deck, wading pool, swimming pool or aquatic recreation pool.1.3.2 Home playground or home pool equipment or play equipment as scoped in Consumer Safety Performance Specification F1148-21 and ANSI/NSPI-5.1.3.3 Waterslides as scoped in Practice F2376-22.1.3.4 Flotation devices used on water slides or in swimming pools.1.3.5 Swimming pools as specified by ANSI/NSPI-1 or ANSI/IAF-9.1.3.6 Products or facility elements specifically designed to provide access to and from pools for people with disabilities.1.3.7 Water rides such as log flumes, raft rides, inner tube rides, waterslides or other attractions where the participant sits in a vehicle or is physically propelled or moved by or with water.1.3.8 Sports equipment, fitness equipment, and diving equipment.1.4 Compliance: 1.4.1 Where water is indirectly or directly added or applied to Consumer Safety Performance Specification F1487-21 play equipment, the equipment shall comply with this standard. Where a requirement for compliance to a section of the Consumer Safety Performance Specification F1487-21 standard is required by this standard, the section number is preceded with the standard's designation.1.4.2 Soft contained play structures with aquatic play components shall comply with Safety Performance Specification F1918-21 except as modified by this standard.1.4.3 Aquatic play components and composite play structures represented, as complying with this safety performance standard shall meet all applicable requirements specified herein. Anyone representing compliance with this standard shall keep such essential records as are necessary to document any claim that the requirements within this standard have been met.1.5 This standard includes the following sections: Section 1Referenced Documents Section 2Terminology Section 3Manufacturing and Materials Section 4Design Section 5Performance Requirements Section 6Operator Responsibilities Section 7Manufacturer/DesignerResponsibilities Section 8Installer Responsibilities Section 91.6 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units that are provided for information only and are not considered standard. (The conversion factor from inch-pound to metric units is 1 in. = 25.4 mm, and 1 lb = 4.4482 N.)1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 646元 / 折扣价： 550 元 加购物车

5.1 A microcosm test is conducted to obtain information concerning toxicity or other effects of a test material on the interactions among three trophic levels (primary, secondary, and detrital) and the competitive interactions within each trophic level. As with most natural aquatic ecosystems, the microcosms depend upon algal production (primary production) to support the grazer trophic level (secondary production), which along with the microbial community are primarily responsible for the nutrient recycling necessary to sustain primary production. Microcosm initial condition includes some detritus (chitin and cellulose) and additional detritus is produced by the system. The microcosms include ecologically important processes and organisms representative of ponds and lakes, but are non-site specific. To the extent possible, all solutions are mixtures of distilled water and reagent grade chemicals (see Section 8) and all organisms are available in commercial culture collections.5.2 The species used are easy to culture in the laboratory and some are routinely used for single species toxicity tests (Guide E729; Practice D3978, Guides E1192 and E1193). Presumably acute toxicity test results with some of these species would be available prior to the decision to undertake the microcosm test. If available, single species toxicity results would aid in distinguishing between indirect and direct effects.5.3 These procedures are based mostly on published methods (4-6), interlaboratory testing (7-10, 11), intermediate studies (12-23, 24), statistical studies (25-27) and mathematical simulation results (28). Newer studies on jet fuels have been reported (29)(See 15.1 for multivariate statistical analyses) and on the implications of multispecies testing for pesticide registration (30). Environmental Protection Agency, (EPA) and Food and Drug Administration, (FDA) published similar microcosm tests (31). The methods described here were used to determine the criteria for Acceptable Tests (Section 16). Additional papers have been published using this method for measuring chemical stress on organisms (32).5.4 Concurrent to measuring the ecological effects, it is advisable to measure the concentration of the parent test chemical, and if possible, the transformation products ((33) see Section 12). The concentrations can be measured on either the same microcosms or on concurrent replicates. Information on the chemical concentrations of parent material and transformation products would aid in the assessment of chemical persistence, exposure, accumulation, and in interpreting, if recovery is associated with chemical degradation or biological adaptation. This protocol deals only with ecological effects, because the techniques for fate studies are in general usage.5.5 In the microcosm, as in natural ecosystems, a population must be able to obtain its requirements from the products of other trophic levels, to maintain a birth rate equal to or greater than its death rate, and to support populations of organisms that will remove its waste products. As in natural ecosystems, several organisms might be capable of fulfilling the same function, and shifts in species dominance can occur without disruption of an ecological process. However, species that are “ecological equivalents” in one function might not be “equivalent” in other functions; for example, a filamentous alga and a single cell alga might equally produce O2, remove NO3, NH3, and PO4, but differ in the type of grazer populations they can sustain, for example, filamentous alga might support amphipods whereas unicellular algae might support Daphnia.5.6 Results of these microcosm tests might be more likely to be indicative of natural ecosystem responses to chemicals than single species toxicity tests because microcosm tests can indicate the explosive population increases that might occur in a community when more sensitive competitors or predators are eliminated or the food supply is increased through competitive interactions. Also, microcosm tests are more likely to display the effects of chemical transformation or increased exposure to certain organisms by means of concentration of parent or degradation products in their food source or habitat.5.7 A list of potential ecological effects is provided to serve as a summary (see Annex A1).5.8 The microcosm test can also be used to obtain information on the toxicity or other effects of species or strains, not included in the control inocula (13). Additional modifications might be required.5.9 Explicit Limitations of the Aquatic Microcosm Protocol: 5.9.1 The scope of the test is limited in the following respects:5.9.1.1 No fish or other vertebrates are included,5.9.1.2 Predation on Daphnia is extremely limited or absent,5.9.1.3 The ecosystem becomes nutrient limited,5.9.1.4 The inocula are not gnotobiotic and aseptic technique is not used (except in maintaining stock cultures of microorganisms). Contaminating microorganisms are likely to be introduced with the larger organisms and during sampling.5.9.1.5 Most detrital processing is carried out by the sediment microbial community, but this community is not clearly described or measured by this protocol.5.9.2 Extrapolation to natural ecosystems should consider differences in community structure, limiting factors, and water chemistry (see Section 17).1.1 This practice covers procedures for obtaining data concerning toxicity and other effects of a test material to a multi-trophic level freshwater community, independent of the location of the test.1.2 These procedures also might be useful for studying the fate of test materials and transformation products, although modifications and additional analytical procedures might be necessary.1.3 Modification of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting multi-trophic level tests.1.4 This practice is arranged as follows:  Section   Referenced Documents 2Terminology 3Summary of Practice 4 5Apparatus 6 Facilities 6.1 Container 6.2 Equipment 6.3Hazards 7Microcosm Components 8 Medium 8.1 Medium Preparation 8.2 Sediment 8.3 Microcosm Assembly 8.4Test Material 9 General 9.1 Stock Solution 9.2 Nutrient Control 9.3Test Organisms 10 Algae 10.1 Animals 10.2 Specificity of Organisms 10.3 Sources 10.4 Algal Culture Maintenance 10.5 Animal Culture Maintenance 10.6Procedure 11 Experimental Design 11.1 Inoculation 11.2 Culling 11.3 Addition of Test Material 11.4 Measurements 11.5 Reinoculations 11.6Analytical Methodology 12Data Processing 13Calculations of Variables from Measurements 14Statistical Analyses 15Acceptability of Test 16Interpretation of Results 17Report 18Annex Annex A1Appendices   Relationship of Media Appendix X1 Statistical Guidance Appendix X21.5 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.Specific hazard statements are given in Section 7.1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 843元 / 折扣价： 717 元 加购物车