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1.1 This method covers quantitative methods for the sampling and determination of Gram-negative bacterial endotoxin concentrations in water miscible metalworking fluids (MWF).1.2 Users of this method should be familiar with the handling of MWF.1.3 This method gives an estimate of the endotoxin concentration of the sampled MWF.1.3.1 Used on site, this method gives an indication of changes in Gram-negative bacterial contamination in the MWF.1.3.2 This method does not replace Practice E 2144.1.4 This method seeks to minimize inter-laboratory variation but does not ensure uniformity of results.1.5 This method is not intended to relate endotoxin concentration in MWF to health effects of inhaled endotoxin.1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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5.1 Certified reference materials (CRMs) prepared from nuclear materials are well characterized, traceable, and sufficiently homogenous and stable for their intended use. Usually they are certified using the most unbiased and precise measurement methods available, often with more than one laboratory being used on a national or international level. CRMs are at the top of the metrological hierarchy of reference materials. A graphical representation of a typical national nuclear measurement system is shown in Fig. 3.FIG. 3 Typical National Nuclear Measurement System5.2 Working reference materials (WRMs) need to have quality characteristics that are similar to CRMs, although the rigor used to achieve those characteristics is not usually as stringent as for CRMs. Similarly, production of WRMs should be in accordance with applicable requirements of ISO 17034. Where possible, CRMs are typically used to calibrate the methods used for establishing reference values assigned to WRMs, thus providing traceability to CRMs as required by ISO/IEC 17025. A WRM is normally prepared for a specific application.5.3 Because of the importance of having highly reliable measurement data from nuclear material analysis, particularly for material control and accountability purposes, CRMs are used for calibration when available. However, CRMs prepared from nuclear materials are not always available for specific applications. Thus, there may be a need for a laboratory to prepare nuclear material WRMs to meet specific needs; for example, to match the matrix in process samples. In such cases, a WRM can be tailored to meet specific needs of a process or laboratory. Also, CRM supply may be too limited for use in the quantities needed for long-term, routine use. When properly prepared, WRMs will serve equally well as CRMs for most applications, and using WRMs will help preserve supplies of CRMs.5.4 Difficulties may be encountered in the preparation of RMs from nuclear materials because of the chemical and physical properties of the materials. Chemical instabilities, problems in ensuring stoichiometry, homogeneity, and radioactivity are among the factors to be considered, with all three factors being involved with some materials. Those preparing WRMs from nuclear materials need to be aware of how these factors may affect preparation, as well as being aware of the other criteria governing the preparation of reliable WRMs.5.5 While use of WRMs provides benefits for the laboratory, it is important to observe the distinction between WRMs, which are prepared by a laboratory for use by that laboratory (or, in some cases, an affiliated satellite laboratory or production facility served by the laboratory), and CRMs which provide certificates of analysis (in accordance with ISO Guide 31) and can be offered for sale.1.1 This guide covers the preparation and characterization of working reference materials (WRM) that are produced by a laboratory for its own use in the analysis of nuclear fuel cycle materials. Guidance is provided for proper planning, preparation, packaging, and storage; requirements for characterization; homogeneity and stability considerations; and value assignment. When traceability to SI is desired for a WRM, it will be achieved by a defined, statistically sound characterization process that is traceable to a certified value on a certified reference materials. While the guidance provided is generic for nuclear fuel cycle materials, detailed examples for some materials are provided in the appendixes.1.2 This guide does not apply to the production and characterization of certified reference materials (CRM). Refer to ISO 17034 and ISO Guide 35 for guidance on reference material production, characterization, certification, sale, and distribution requirements.1.3 The information provided by this guide is found in the following sections:  SectionPerform WRM Planning 6Prepare and Process Materials 7Packaging and Storage of Materials 8Perform Homogeneity Study 9Perform Stability Studies 10Characterize Materials 11Perform Uncertainty Analysis 12Produce Documentation 13Carry Out WRM Utilization and Monitoring 141.4 The values stated in SI units are to be regarded as standard. The non-SI units of molar, M, and normal, N, are also regarded as standard. Any non-SI units of measurement shown in parentheses are for information only.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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