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3.1 The electrical sensing zone method for cell counting is used in tissue culture, government research, and hospital, biomedical, and pharmaceutical laboratories for counting and sizing cells. The method may be applicable to a wide range of cells sizes and cell types, with appropriate validation (10).3.2 The electrical sensing zone methodology was introduced in the mid-1950s (9). Since this time, there have been substantial improvements which have enhanced the operator's ease of use. Among these are the elimination of the mercury manometer, reduced size, greater automation, and availability of comprehensive statistical computer programs.3.3 This instrumentation offers a rapid result as contrasted to the manual counting of cells using the hemocytometer standard counting chamber. The counting chamber is known to have an error of 10 to 30 %, as well as being time-consuming (11). In addition, when counting and sizing porcine hepatocytes, Stegemann et al concluded that the automated, electrical sensing zone method provided greater accuracy, precision, and speed, for both counts and size, compared to the conventional microscopic or the cell mass-based method (7).1.1 This test method, provided the limitations are understood, covers a procedure for both the enumeration and measurement of size distribution of most all cell types. The instrumentation allows for user-selectable cell size settings and is applicable to a wide range of cell types. The method works best for spherical cells, and may be less accurate if cells are not spherical, such as for discoid cells or budding yeast. The method is appropriate for suspension as well as adherent cell cultures (1).2 Results may be reported as number of cells per milliliter or total number of cells per volume of cell suspension analyzed. Size distribution may be expressed in cell diameter or volume.1.2 Cells commonly used in tissue-engineered medical products (2) are analyzed routinely. Examples are chondrocytes (3), fibroblasts (4), and keratinocytes (5). Szabo et al. used the method for both pancreatic islet number and volume measurements (6). In addition, instrumentation using the electrical sensing zone technology was used for both count and size distribution analyses of porcine hepatocytes placed into hollow fiber cartridge extracorporeal liver assist systems. In this study (7), and others (6, 8), the automated electrical sensing zone method was validated for precision when compared to the conventional visual cell counting under a microscope using a hemocytometer. Currently, it is not possible to validate cell counting devices for accuracy, since there not a way to produce a reference sample that has a known number of cells. The electrical sensing zone method shall be validated each time it is implemented in a new laboratory, it is used on a new cell type, or the cell counting procedure is modified.1.3 Electrical sensing zone instrumentation (commonly referred to as a Coulter counter) is manufactured by a variety of companies and is based upon electrical impedance. This test method, for cell counting and sizing, is based on the detection and measurement of changes in electrical resistance produced by a cell, suspended in a conductive liquid, traversing through a small aperture (see Fig. 1(9)). When cells are suspended in a conductive liquid, phosphate-buffered saline for instance, they function as discrete insulators. When the cell suspension is drawn through a small cylindrical aperture, the passage of each cell changes the impedance of the electrical path between two submerged electrodes located on each side of the aperture. An electrical pulse, suitable for both counting and sizing, results from the passage of each cell through the aperture. The path through the aperture, in which the cell is detected, is known as the “electronic sensing zone.” This test method permits the selective counting of cells within narrow size distribution ranges by electronic selection of the generated pulses. While the number of pulses indicates cell count, the amplitude of the electrical pulse produced depends on the cell's volume. The baseline resistance between the electrodes is due to the resistance of the conductive liquid within the boundaries of the aperture. The presence of cells within the “electronic sensing zone” raises the resistance of the conductive pathway that depends on the volume of the cell. Analyses of the behavior of cells within the aperture demonstrates that the height of the pulse produced by the cell is the parameter that most nearly shows proportionality to the cell volume.1.4 Limitations are discussed as follows:1.4.1 Coincidence—Occasionally, more than a single cell transverses the aperture simultaneously. Only a single larger pulse, as opposed to two individual pulses, is generated. The result is a lower cell count and higher cell volume measurement. The frequency of coincidence is a statistically predictable function of cell concentration that is corrected by the instrument. This is called coincidence correction (8). This phenomenon may be reduced by using lower cell concentrations.1.4.2 Viability—Electrical sensing zone cell counting enumerates both viable and nonviable cells and cannot determine percent viable cells. A separate test, such as Trypan blue, is required to determine percent viable cells.1.4.3 Cell Diameter—This is a function of the size range capability of the aperture size selected. Measurements may be made in the cell diameter range of 0.6 μm to 1200 μm. Setting the counting size range on the instrument can affect the test results, especially if the cell size has a large distribution, and should be carefully controlled to help achieve repeatability.1.4.4 Size Range of the Aperture—The size range for a single aperture is proportional to its diameter. The response has been found to depend linearly on diameter over a range from 2 % to 80 % of the diameter. However, the aperture tube may become prone to blockage at levels greater than 60 % of diameter. Therefore, the practical operating range of the aperture is considered to be 2 % to 60 % of the diameter.1.4.5 Humidity—10 % to 85 %.1.4.6 Temperature—10 °C to 35 °C.1.4.7 Electrolyte Solution—The diluent for cell suspension shall provide conductivity and have minimal effect on cell size. The electrolyte of choice is commonly phosphate-buffered saline.

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ASTM D6703-19 Standard Test Method for Automated Heithaus Titrimetry Active 发布日期 :  1970-01-01 实施日期 : 

5.1 This test method is intended as a laboratory diagnostic tool for estimating the colloidal stability of bitumen asphalt, asphalt cross blends, aged asphalt, and heavy oil residuum. Historically, bituminous asphalt and heavy oil residua have been modeled as colloidal suspensions in which a polar associated asphaltene moiety (the dispersed phase) is suspended in a maltene solvent moiety (the dispersing medium) (refer to Test Methods D3279 and D4124 for further definition of asphalt fraction materials). The extent to which these two moieties remain in state of peptization is a measure of the compatibility (colloidal stability) of the suspension. Compatibility may influence the physical properties of these materials, including rheological properties, for example, phase angle and viscosity. This test method and other similar test methods, along with the classical Heithaus test, may be recommended as a measure of the overall compatibility of a colloidal system by determining a parameter referred to as the state of peptization, P. The value of P commonly varies between 2.5 to 10 for unmodified or neat asphalts. Materials calculated to have low values of P are designated incompatible. Materials calculated to have high P values are designated compatible. Values in P are calculated as a function of two parameters that relate to the peptizability of the asphaltene moiety (the asphaltene peptizability parameter, pa) and the solvent power of the maltene moiety (the maltene peptizing power parameter, po). Values of pa and po are calculated as functions of the quantities Cmin and FRmax. Values of Cmin and FRmax are determined from experimental variables, the weight of asphalt (Wa), the volume of solvent (VS) to dissolve the weight of asphalt, and the volume of titrant (VT) added to initiate flocculation.1.1 This test method describes a procedure for quantifying three Heithaus compatibility parameters that quantify the colloidal stability of asphalts and asphalt cross blends and aged asphalts.1.2 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.3 The text of this standard references notes and footnotes which provide explanatory material. These notes and footnotes (excluding those in tables and figures) shall not be considered as requirements of the standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 This test method is useful for accurate measurement of from a wide variety of glass samples, whose ranges from 1.48–1.55. 4.2 It should be recognized that measurement of surface fragments, especially from float glass samples, can result in refractive index values which are different than the refractive index values of fragments from the interior of (for example, bulk) the same broken glass source (5). 4.3 The precision of this test method shall be established in each laboratory that employs it as part of the validation protocol (see Section 9). 4.4 It should be recognized that this technique measures the refractive index of the glass at the match point temperature, which will be higher than ambient temperature, and thus, may give different values from those obtained by other methods, which measure the refractive index at room temperature. 1.1 This test method covers a procedure for measuring and comparing the refractive index (η) at a fixed wavelength (λ) and temperature (T) ( ) of glass from known sources to recovered fragments from a questioned source. 1.2 This test method does not include the measurement of optical dispersion or the measurement of refractive index ( ) at any other wavelength other than the Sodium D line ( ). This method employs a narrow band pass filter at 589 nm, but other filters could be employed using the described method, allowing the to be determined at other wavelengths, and therefore, also allowing for the dispersion value to be calculated. 1.3 Alternative methods for the determination of are listed in Refs (1-5).2 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard cannot replace knowledge, skills, or abilities acquired through education, training, and experience and is to be used in conjunction with professional judgment by individuals with such discipline-specific knowledge, skills, and abilities. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 Residue in LPG is a contaminant that can lead to operational problems in some end use applications. Engines, micro-turbines, fuel cells and other equipment may be sensitive to residue levels as low as 10 mg/kg.5.2 Contamination of LPG can occur during production, transport, delivery, storage and use. A qualitative indication of the contaminants can help track down the source of the contamination from manufacture, through the distribution system, and to the end user.5.3 This test method is designed to provide a lower detection limit, wider dynamic range, and better accuracy than gravimetric methods like Test Method D2158.5.4 This test method can be performed with little or no discharge of LPG vapors, compared to Test Method D2158 which requires evaporation of 100 mL of sample per test.5.5 Sampling for residue in LPG using sorbent tubes can be performed in the field, and the sorbent tubes sent to a laboratory for analysis. This saves significant costs in shipping (weight of tube is approximately 10 grams), and is much safer and easier than transporting LPG cylinders.5.6 This test method determines total residues from C6 to C40, compared to a thermal gravimetric residue method such as Test Method D2158 which heat the residue to 38°C, resulting in a lower recovery due to loss of lighter residue components.5.7 If there is a need to decrease the detection limit of residue or individual compounds of interest below 10 µg/g, the procedures in this test method can be modified to achieve 50 times enhanced detection limit, or 0.2 µg/g.1.1 This test method covers the determination of residue in LPG by automated thermal desorption/gas chromatography (ATD/GC) using flame ionization detection (FID).1.2 The quantitation of residue covers a component boiling point range from 69°C to 522°C, equivalent to the boiling points of C6 through C40 n-paraffins.1.2.1 The boiling range covers possible LPG contaminants such as gasoline, diesel fuel, phthalates and compressor oil. Qualitative information on the nature of the residue can be obtained from this test method.1.2.2 Materials insoluble in LPG and components which do not elute from the gas chromatograph or which have no response in a flame ionization detector are not determined.1.2.3 The reporting limit (or limit of quantitation) for total residue is 6.7 µg/g.1.2.4 The dynamic range of residue quantitation is 6.7 to 3300 µg/g.1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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Cyanides are known to be toxic to man, but more so to fish and other aquatic life. The complexity of the chemistry of cyanides has led to the coexistence of several cyanide species in the environment. The presence of cyanides in industrial, domestic, and surface water is cause for concern. Several regulations and standards require continuous monitoring of cyanides in different types of water and wastes. The automated test methods presented offer useful tools for such monitoring. (See also Practice D 4193.)1.1 These test methods cover the determination of different species of cyanides and thiocyanate in water and waste water, namely weak acid dissociable cyanide, total cyanide, and thiocyanate ().1.1.1 Total Cyanide This test method determines all the weak acid dissociable cyanides and the strong metal-cyano-complexes, such as ferrocyanide [Fe(CN)6] 4, ferricyanide [Fe(CN)6]3, hexacyanocolbaltate [Co(CN) 6]3, and those of gold and platinum.1.1.2 Weak Acid Dissociable CyanideThis test method basically determines free cyanides, as CN and HCN, and weak metal-cyano-complexes such as [Cd(CN) 4] 2 and [Mn(CN)6] 3. Iron complexes are not included.1.1.3 Strong cyanide complexes, like those of iron, cobalt, etc., can be determined by difference, that is, cyanide complexes = total cyanides weak acid dissociable cyanides.1.1.4 Thiocyanate This test method determines the thiocyanate as the difference between another measurement that includes total cyanide plus thiocyanate and the value of total cyanide, that is, thiocyanate = total cyanide plus thiocyanate total cyanide.1.2 Cyanates and cyanogen halides are not detected. Cyanogen chloride hydrolyzes to cyanate at the pH of sample preservation (12).1.3 Most of the organo-cyano-complexes are not measured, with the exception of the weak cyanohydrins.1.4 These test methods apply to different types of water, waste water (raw sewage, sludge, and effluent), sludge, some industrial waste, and sediments. Sample matrixes should be evaluated by the user. The reported precision and bias (see Section ) may not apply to all samples.1.5 The values stated in SI units are to be regarded as the standard.This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. For specific precautionary statements, see Section 9.

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5.1 This test method allows for the measurement of the torque retention properties of container/continuous thread closure systems of various designs, materials, and manufacture, and is suitable for packaging development and engineering evaluation.5.2 This test method can be used for the evaluation of container/continuous thread closure systems under controlled conditions (where the application torque is known and the applied downward force to the closure is zero).5.3 This test method measures torque retention properties of container/continuous thread closure systems with the use of a non-automated, spring torque-meter (with either a dial indicator or a digital readout) or a torque wrench.1.1 These test methods evaluate the torque retention of continuous thread closures on containers, with matching finishes, for predetermined environmental conditions over time.1.2 The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system may not be exact equivalents; therefore, each system shall be used independently of the other. Combining values from the two systems may result in non-conformance with the standard.NOTE 1: The SI unit system is the recommended system.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 Many petroleum products and some non-petroleum products are used as lubricants in the equipment, and the correct operation of the equipment depends upon the appropriate viscosity of the lubricant being used. Additionally, the viscosity of many petroleum fuels is important for the estimation of optimum storage, handling, and operational conditions. Thus, the accurate determination of viscosity is essential to many product specifications.5.2 The viscosity of used oils is a commonly determined parameter in the oil industry to assess the effect of engine wear on the lube oils used, as well as the degradation of the engine parts during operation.5.3 The Houillon viscometer tube method offers automated determination of kinematic viscosity. Typically a sample volume of less than 1 mL is required for the analysis.1.1 This test method covers the measurement of the kinematic viscosity of transparent and opaque liquids; such as base oils, formulated oils, diesel oil, biodiesel, biodiesel blends, residual fuel oils, marine fuels, and used lubricating oils using a Houillon viscometer in automated mode.1.2 The range of kinematic viscosity capable of being measured by this test method is from 2 mm2/s to 2500 mm2/s (see Fig. 1). The range is dependent on the tube constant utilized. The temperature range that the apparatus is capable of achieving is between 20 °C and 150 °C, inclusive. However, the precision has only been determined for the viscosity range; 2 mm2/s to 478 mm2/s at 40 °C for base oils, formulated oils, diesel oil, biodiesel, and biodiesel blends; 3 mm2/s to 106 mm2/s at 100 °C for base oils and formulated oils; 25 mm2/s to 150 mm2/s at 40 °C and 5 mm2/s to 16 mm2/s at 100 °C for used lubricating oils; 25 mm2/s to 2500 mm2/s at 50 °C and 6 mm2/s to 110 mm2/s at 100 °C for residual fuels. As indicated for the materials listed in the precision section.FIG. 1 Houillon Viscometer Typical Viscosity Range of Tube ConstantsNOTE 1: Viscosity range of a Houillon tube is based on most practical flow time of 30 s to 200 s.1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For specific warning statements, see Section 7.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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This specification establishes the performance-based and prescriptive-based methods of evaluating various classes of automated gate constructions that are used for vehicular traffic. The gate types addressed in this specification include horizontal slide gates, horizontal swing gates, vertical lift gates, vertical pivot gates, and overhead pivot gates. Conversely, the four classes of gates covered here are as follows: Class I, a gate for the garage or parking area intended for use in a home of a one-to-four single family dwelling; Class II, a gate intended for use in a commercial location or building such as a multi-family housing unit (five or more single family units), hotel, garages, retail store, or other building servicing the general public; Class III, a gate intended for use in an industrial location or building such as a factory, loading dock area, or other locations not intended to service the general public; and Class IV, a gate intended for use in a guarded industrial location or building such as an airport security area, or other restricted access locations not servicing the general public, in which unauthorized access is prevented by means of supervision by security personnel.1.1 This specification defines performance-based and prescriptive-based methods of evaluating various classes of gates that are used for vehicular traffic and are to be automated.1.2 Gate types addressed in this specification include horizontal slide gates, horizontal swing gates, vertical lift gates, vertical pivot gates, and overhead pivot gates.1.3 Gate types not listed in this specification will be subject to any applicable provisions contained in this specification.1.4 Automated vehicular gate systems shall comply with this specification and shall be compliant with UL 325.1.5 Units—The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units that are provided for information only and are not considered standard.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 Shot peening is a process for cold working surfaces by bombarding the product with shot of a solid and spherical nature propelled at a relatively high velocity. In general, shot peening will increase the fatigue life of a product that is subject to bending or torsional stress. It will improve resistance to stress corrosion cracking. It can be used to form parts or correct their shapes. See Appendix X1 for additional information.5.2 It is essential that the shot peening process parameters be controlled rigidly to ensure repeatability from part to part and lot to lot.5.3 This specification covers techniques and methods necessary for proper control of the shot peening process.AbstractThis specification covers the requirements for automated, controlled shot peening of metallic articles prior to electrolytic or autocatalytic deposition of nickel or chromium, or as a final finish, using shot made of cast steel, conditioned cut wire, or ceramic media. The process is applicable to those materials on which test work has shown it to be beneficial within given intensity ranges. It is not suitable for brittle materials. Hand peening and rotary flap peening are excluded specifically. Shot peening induces residual compressive stresses in the surface and near-surface layers of metallic articles, controlling or limiting the reduction in fatigue properties that occurs from nickel or chromium plating of the article, or the fatigue properties of unplated articles. It is a process for cold working surfaces by bombarding the product with shot of a solid and spherical nature propelled at a relatively high velocity. Cast steel, cut wire, and ceramic shot shall all be spherical in shape and shall all be free of sharp edges, corners, and broken pieces. Prior to shot peening, the following operations shall be done first: heat treatment, machining, grinding, flaw test, crack test, corrosion detection, cleaning, and masking. Peened surfaces shall be uniform in appearance and completely dented so that the original surface is obliterated entirely. After shot peening, the following methods shall be done: residual shot removal, surface finishing, chemical cleaning of nonferrous metals and their alloys, thermal and heat treatments, and corrosion protection.1.1 This specification covers the requirements for automated, controlled shot peening of metallic articles prior to electrolytic or autocatalytic deposition of nickel or chromium, or as a final finish, using shot made of cast steel, conditioned cut wire, or ceramic media. The process is applicable to those materials on which test work has shown it to be beneficial within given intensity ranges. It is not suitable for brittle materials. Hand peening and rotary flap peening are excluded specifically.1.2 Shot peening induces residual compressive stresses in the surface and near-surface layers of metallic articles, controlling or limiting the reduction in fatigue properties that occurs from nickel or chromium plating of the article, or the fatigue properties of unplated articles.1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 For hydrocarbon resins and rosin based resins, softening does not take place at a definite temperature. As the temperature rises, these materials gradually change from brittle solids or very viscous liquids to less viscous liquids. For this reason, determination of the softening point must be made by a fixed, arbitrary, and closely defined method if the results obtained are to be comparable.1.1 These test methods are intended for determining the softening point of hydrocarbon resins, rosin based resins and similar materials by means of an automated ring-and-ball apparatus. Portions are similar in technical content to the automated-apparatus versions of Test Methods D36, E28, and ISO 4625.1.1.1 The ring-and-ball softening point of a hydrocarbon resin and rosin based resins may also be determined with lower precision using the manual ring-and-ball softening point procedure in Test Methods E28.1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This test method allows for the measurement of the torque retention properties of container/continuous thread closure systems of various designs, materials, and manufacture, and is suitable for package development and engineering evaluation.5.2 Each test method can be used for the evaluation of non child resistant container/continuous thread closure systems under controlled conditions such as when the application torque is known and the applied downward force to the closure is zero or for Type I, style “A” push down and turn child resistant container/continuous thread closure systems under controlled conditions such as when the application torque and the applied downward force to the closure is known.5.3 This test method measures torque retention properties of container/continuous thread closure systems with the use of an automated transducer based torque meter operating at a known rotational velocity (rpm) or known torque ramp.5.4 This test method is intended for measurement of dry torque only.1.1 These test methods evaluate the torque retention of continuous thread closures on containers with matching finishes, for predetermined environmental conditions over time. Methods are defined for both Type I, style “A” push down and turn Type II2 child resistant and non child resistant type closures.1.2 The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system may not be exact equivalents; therefore, each system shall be used independently of the other. Combining values from the two systems may result in non-conformance with the standard.NOTE 1: The SI unit system is the recommended system.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 This test method is used for quantitative determination of asphalt binder in asphalt mixtures and asphalt pavement samples for specification acceptance, service evaluation, control, and research.4.2 Aggregates obtained by this method may be used for sieve analysis using Test Method D5444. Extracted asphalt binder from this test method may be recovered using Test Method D1856 or Practice D5404/D5404M.NOTE 1: The quality of results produced by this standard is dependent on the competence of the personnel performing the procedure and the capability, calibration, and maintenance of the equipment used. Agencies that meet the criteria of Specification D3666 are generally considered capable of competent and objective testing, sampling, inspection, etc. Users of this standard are cautioned that compliance with Specification D3666 alone does not completely ensure reliable results. Reliable results depend on many factors; following the suggestions of Specification D3666 or some similar acceptable guidance provides a means of evaluating and controlling some of those factors.1.1 This test method covers the quantitative determination of asphalt binder content in asphalt mixtures and pavement specimens, using the automated computer controller or human-machine interface system (HMI), to perform a solvent extraction for specification acceptance, service evaluation, quality control, and research.1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.3 The text of this standard references notes and footnotes which provide explanatory material. These notes and footnotes (excluding those in tables and figures) shall not be considered as requirements of the standard.1.4 An ILS is being conducted according to Practice E691 and will be available on or before December 2018. Therefore, this standard should not be used for acceptance or rejection of a material for purchasing purposes.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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