5.1 This practice describes a procedure for producing spore suspensions of C. difficile ATCC 700792, C. difficile ATCC 43598, or C. difficile ATCC 43599. The spore suspensions may be used in antimicrobial efficacy testing, or other laboratory testing requiring C. difficile spores. A spore crop is considered acceptable if the titer is >8 log10 spores/mL, purity of 95 %, and is resistant to 2.5M HCl after 10 min of exposure.1.1 This practice is designed to propagate spores of Clostridioides difficile using liver broth.1.2 It is the responsibility of the user of this practice to determine whether Good Laboratory Practices are required and follow when appropriate.1.3 This practice should only be performed by those trained in microbiological techniques.1.4 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 Core sampling is an acceptable way of obtaining a test specimen without destroying the usefulness of an anode block.4.1.1 Test specimen obtained by this guide can be used by producers and users of carbon anodes for the purpose of conducting the tests in Note 1 to obtain comparative physical properties.4.2 Sampling shall not weaken the anode or increase the likelihood of premature failure.1.1 This guide covers sampling for prebaked carbon anodes used in the production of aluminum, and details procedures for taking test samples from anode blocks. It covers equipment and procedures for obtaining samples from anode blocks in a manner that does not destroy the block or prevent its subsequent use as originally intended. However, the user must determine the subsequent use of the sampled anode blocks. Preferred locations for taking samples from single units of anodes are covered in this guide.1.1.1 Information for sampling of shaped refractory products, in general, is given in ISO 5022. This standard details the statistical basis for sampling plans for acceptance testing of a consignment or lot. Anodes used in the production of aluminum have specific requirements for sampling and while the statistical basis for sampling given in ISO 5022 applies, further or modified requirements may also apply.1.1.2 Information for sampling of anodes for Al-metal production is given in ISO 8007-2. This standard details the statistical basis for sampling plans for acceptance testing of a consignment or lot.1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.NOTE 1: The following ASTM standards are noted as sources of useful information: Test Methods D5502, D6120, D6744, and D6745.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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This specification covers production acceptance test requirements for the manufacture of lighter-than-air light sport aircraft. This provides the minimum requirements for the establishment of a ground and flight test program for verifying the initial (first run) production aircraft meets certain operational performance requirement that have been set forth by the manufacturer in its Aircraft Operating Instructions. The minimum requirements to verify that each subsequent production aircraft has no obvious defects that would prevent the safe operation of the aircraft are described. Every production aircraft shall be flight tested to the minimums such as lighter-than-air airships, hot-air balloons and gas/hybrid balloons.1.1 This specification covers production acceptance test requirements for the manufacture of lighter-than-air light sport aircraft.1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 Core sampling is an acceptable way of obtaining a test specimen without destroying the usefulness of a cathode block.4.1.1 Test specimens obtained by this guide can be used by producers and users of cathode blocks for the purpose of conducting the tests in Note 1 to obtain comparative physical properties.4.2 Sampling shall not weaken the cathode block or increase the likelihood of premature failure. Extreme care shall be exercised when taking vertically drilled samples.1.1 This guide covers sampling of carbon cathode blocks used in the production of aluminum, and details procedures for taking samples from single cathode blocks. It covers equipment and procedures for obtaining samples from cathode blocks in a manner that does not destroy the cathode block or prevent its subsequent use as originally intended. However, the user must determine the subsequent use of the sampled cathode blocks. Preferred locations for taking samples from single units of cathode blocks are covered in this guide.1.1.1 Information for sampling of shaped refractory products, in general, is given in ISO 5022. This standard details the statistical basis for sampling plans for acceptance testing of a consignment or lot. Cathode blocks used in the production of aluminum have specific requirements of sampling, and while the statistical basis for sampling given in ISO 5022 applies, further or modified requirements may also apply.1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.NOTE 1: The following ASTM standards are noted as sources of useful information: Test Methods C559, C611, C651, C747, C1025, C1039, and C1225.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 This document provides guidance for the implementation of procedures for the preparation, testing, and documentation of an in-house reference material (hereafter called an iRM) to be used for a number of purposes, enumerated in the following document, associated with development, validation, and control of chemical and physical measurement processes.4.2 This guide is primarily concerned with characterization of the chemical compositions of metals, ores, and related materials. For all these materials, there is a continuing, strong demand for reference materials (RMs) that is difficult for metrology institutes and private certified reference material (CRM) developers to meet because CRM development requires substantial investments of time and money. The metals and mining industries consume RMs and create new product and by-product compositions at high rates. They use analytical methods that provide rapid and accurate determinations, and both quality assurance and quality control can be maintained using efficient procedures provided appropriate iRMs are available.4.3 The user of this guide must recognize that development of an iRM for any purpose carries with it the responsibility to design and execute the development process correctly, and to document the process thoroughly. In addition, the user of an iRM bears the responsibility for correct use of the iRM material within its design limitations.4.4 This guide contains discussions on material selection and sampling for RMs with some attention given to conversion to the final forms.4.5 The use of iRMs is appropriate for control chart procedures to demonstrate that chemical measurement processes are under statistical control. This function requires demonstration of sufficient homogeneity of a material, but it does not require assignment of chemical and physical property values with associated, exhaustively evaluated uncertainties.4.6 The use of iRMs is appropriate for calibration of test methods and evaluation of calibrations in several ways, including checking for bias, systematic testing of corrections for matrix effects, and testing of sample preparation procedures. See Section 6. This guide provides explanations of general cases in which an iRM can be used as part of a validation process (see Guide E2857).4.7 Because this document is a standard guide, it is intended to educate those who are involved in laboratory operation, quality system development and maintenance, and accreditation of laboratory operations within the scope of a quality system. However, this guide does not constitute requirements for assessment and accreditation.1.1 This document provides guidance for the implementation of procedures for preparation of in-house reference materials for analytical testing of metals, ores, slags, and other materials encountered within the metals and mining industries.1.2 This guide is applicable to the production of reference materials only (usually for internal use) and does not apply to the production of certified reference materials (CRMs). Materials may include metals, alloys, minerals, geological materials, manufacturing intermediates, and byproducts. Samples may be in a number of physical forms including blocks, disks, rods, wires, chips, granules, powders, and liquids.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 A supply well provides groundwater for household, domestic, commercial, agricultural, or industrial uses.4.2 Using a standardized protocol based on an existing industry standard or approved regulatory methods and procedures to collect water samples from a supply well is essential to obtain representative water quality data. These data can be critical to efforts to protect water uses, and human health, and identify changes when they occur. Use of this guide will help the project team to design and execute an effective water supply sampling program.4.3 It is important to understand the objectives of the sampling program before designing it. Water supplies may be sampled for various reasons including any or all of the following:(1) baseline sampling before an operation of concern,(2) periodic sampling during such an operation,(3) investigative responses to perceived changes in water quality, or(4) ongoing monitoring related to known or potential groundwater constituents of concern in the area.Sampling programs should be based on these objectives and be developed in coordination with the prospective laboratory(ies) to ensure its procedures, capabilities, and limitations meet the needs of the program, protect human health and fulfill regulatory requirements.1.1 This guide presents a methodology for obtaining representative groundwater samples from domestic or commercial water wells that are in proximity to oil and gas exploration and production (E&P) operations. E&P operations include, but are not necessarily limited to, site preparation, drilling, completion, and well stimulation (including hydraulic fracturing), and production activities. The goal is to obtain representative groundwater samples from domestic or commercial water wells that can be used to identify the baseline groundwater quality and any subsequent changes that may be identified. While this guide focuses on baseline sampling in conjunction with oil and gas E&P activities, the principles and practices recommended are based on well-established methods that have been in use for many years in other industrial situations. This guide recommends sampling and analytical testing procedures that can identify various chemical species present including metals, dissolved gases (such as methane), hydrocarbons (and other organic compounds), as well as overall water quality.1.2 This guide provides information on typical residential and commercial water supply well systems and guidance on developing and implementing a sampling program, including determining sampling locations, suggested purging techniques, selection of potential analyses and laboratory certifications, data management, and integrity. It also includes guidance on personal safety. The information included pertains to baseline sampling before beginning any activities that could present potential risks to local aquifers, periodic sampling during and after such work, and ongoing monitoring relating to known or potential groundwater constituents in the area. This guide does not address policy issues related to frequency or timing of sampling or sampling distances from the wellhead. In addition, it does not address reporting limits, sample preservation, holding times, laboratory quality control, regulatory action levels, or interpretation of analytical results.1.3 These guidelines are not intended to replace or supersede regulatory requirements and technical methodology or guidance nor are these guidelines intended for inclusion by reference in regulations. Instances where this guide is in conflict with statutory or regulatory requirements, practitioners shall defer to the latter. These guidelines are intended to assist in developing sampling programs to meet project goals and objectives. However, site-specific conditions, regulatory requirements, site-specific health and safety issues, technical manuals and directives, and program data quality objectives should be evaluated and consulted along with the information contained in this guide for each individual site and sampling program.1.4 This guide offers an organized collection of information or a series of options and does not recommend a specific course of action. This document cannot replace education or experience and should be used in conjunction with professional judgment. Not all aspects of this guide may be applicable in all circumstances. This ASTM standard is not intended to represent or replace the standard of care by which the adequacy of a given professional service must be judged, nor should this document be applied without consideration of a project’s many unique aspects. The word “Standard” in the title of this document means only that the document has been approved through the ASTM consensus process.1.5 Users are responsible for investigating and identifying all the legal and regulatory requirements that are applicable for the location where the sampling is being performed.1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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2.1 The purpose of this practice is to establish that each production aircraft has been assembled properly and operates normally throughout its entire allowable flight envelope.2.2 If final assembly is completed outside of the main production facility, the manufacturer shall specify the required procedures and what persons are authorized to perform them.1.1 The following requirements apply for the manufacture of weight-shift-control aircraft. This practice includes the production acceptance test requirements for weight-shift-control aircraft.1.2 This practice applies to aircraft seeking civil aviation authority approval, in the form of flight certificates, flight permits, or other like documentation.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory requirements prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This test method is designed to evaluate nanomaterial capacity to induce nitric oxide production by macrophages.5.2 Activated macrophages generate large quantities of NO. NO generated from activated macrophages is a cytostatic/cytotoxic agent (3-6).5.3 The production of NO in excessive amounts leads to the generation of peroxynitrite by its spontaneous reaction with superoxide. Peroxynitrite causes tissue injury through its capability to damage lipids, proteins, and DNA (2).5.4 NO is a proinflammatory mediator and it is an important marker for activation of inflammation (5, 6).5.5 Testing the capacity of a nanomaterial to induce NO production in vitro helps in predicting the nanomaterial’s biocompatibility through anticipating and understanding the potential problems that might be encountered during its in vivo administration.1.1 This test method delivers a protocol for a quantitative measure of nitrite (NO2–), a stable end-product of nitric oxide (NO), in cell culture medium due to exposure to nanomaterial(s).1.2 NO has a critical role in several pathological conditions in addition to its role in many physiological processes.1.3 This test method uses murine macrophage cell line RAW 264.7 as an in vitro model.1.4 The nitrite is measured in the cell culture medium by a colorimetric analysis using Griess reagent as shown in Fig. 1.FIG. 1 Summary of Nitric Oxide Production Assay1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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1.1 This specification delineates procedural requirements for the audio visual and production services associated with meetings, events, trade shows, or conferences (hereafter, referred to as events).1.2 Audio visual (AV) and production services encompass the range of services required to provide staging, d�cor, scenic elements, audio, video, lighting, and technical production.1.3 There are nine specifications covering the following areas of event planning: destination selection, accommodations, food and beverage, audio visual, onsite offices, communication and marketing materials, transportation, exhibits, and venues. Within each specification are eight categories’ criteria: staff management policy, communications, waste management, energy, air quality, water, procurement, and community partners. Each category is further split into four progressive levels of achievement. Information within each specification is divided as follows: Main Body, Referenced Documents, Terminology, Planner Requirements for Level 1, Supplier Requirements for Level 1, and Keywords; AnnexesPlanner Requirements for Levels 2 – 4 and Supplier Requirements for Levels 2 – 4.1.4 All areas of performance under this specification may not be relevant for each event or for each facility or provider of event services. In some cases, an event planner would use this specification in conjunction with other specifications (for example, Specifications , ) or this specification may be used individually. In other words, elements of an event may be separated and considered solely or added together and considered as a unit (for example, venues used individually versus transportation, accommodations, and destination selection used in conjunction).1.5 If higher levels are achieved in some sections, the event can only be considered environmentally sustainable at the lowest level achieved.1.6 The use of levels within this specification recognizes the path to environmentally sustainable events is a multi-stage effort. The idea behind the levels is to provide a coherent baseline for measuring environmental performance, to be flexible enough to adapt to the needs of more advanced planners and to establish a performance path that encourages further environmental performance improvement. In addition, the evolution of products and services to support events is expected, allowing additional advancements and underscoring the dynamic nature of environmental sustainability. This specification primarily focuses on environmental sustainability, while some aspects of economic and social sustainability are included recognizing the complete path toward overall sustainability.1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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4.1 The purpose of this practice is to provide the minimum requirements necessary for the establishment of a production acceptance program for a manufacturer of light sport aircraft.4.2 The purpose of this specification is to provide the minimum requirements for the establishment of a ground and flight test program for verifying the initial production aircraft meets certain operational performance requirements that have been set forth by the manufacturer in its Pilot’s Operating Handbook (POH).4.3 In addition, this specification provides minimum requirements to verify that each subsequent production airplane has no obvious defects that would prevent the safe operation of the airplane.4.4 All requirements given in this specification are to be performed in accordance with the manufacturer’s Specification F2972-compliant quality assurance system requirements.4.5 The following criteria should not be construed as requirements for specific features to be included on a LSA. When a requirement specifies a feature that does not exist on a LSA, the requirement does not apply.1.1 The following requirements apply for the manufacture of fixed wing aircraft, including gliders. This practice includes the production acceptance test requirements.1.2 This practice applies to aircraft seeking civil aviation authority approval, in the form of flight certificates, flight permits, or other like documentation.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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This specification covers the procedures and test methods for the production of dry cast concrete, defined as very low slump or zero-slump concrete that requires continuous and intense vibration, or mechanical means, or a combination of vibration and mechanical means to consolidate the concrete, enabling immediate removal of the forms from the product. The placement, consolidation, curing, or protection of the concrete are not covered by this specification. The aggregates shall be sized, graded, proportioned, and mixed with such proportions of cementitious material, water, and admixtures, if any, to produce a thoroughly mixed concrete of such quality that the precast product will conform to the test and design requirements of the relevant specification. Materials covered by this specification are the following: cementitious materials (cement, slag cement, fly ash), allowable combinations of cementitious materials, aggregates, and admixtures and blends.This specification also covers weigh batching and mixing, volumetric batching and continuous mixing, reporting, and strength of the specimen.1.1 This specification covers the production of dry cast concrete as defined in 3.2.1. Requirements for quality of concrete shall be as hereinafter specified. This specification does not cover the placement, consolidation, curing, or protection of the concrete.1.2 The values stated in inch-pound units are to be regarded as standard. No other units of measurement are included in this standard.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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1.1 This classification system covers thermoplastic materials, used as joining materials, for the creation of joints between similar or dissimilar materials, to produce finished products or parts that are intended to be disassembled and/or reassembled.1.2 This class of materials enables disconnection of the different parts or layers of a products for refurbishing, repair and full recovery and recycling of the joined materials, for instance at the end of life of the product(s), enabling the reuse of valuable resources, and hence reducing adverse impact on the environment.1.3 The properties included in this classification system are those required to identify the materials covered. It is possible that there are other requirements necessary to identify particular characteristics important to specialized applications. One way of specifying them is by using the suffixes as given in Section 5.1.4 This classification system and subsequent line callout (specification) are intended to provide a means of calling out plastic materials used in the fabrication of end items or parts. It is not intended for the selection of materials. Material selection is best made by those having expertise in the plastic field after careful consideration of the design and the performance required of the part, the environment to which it will be exposed, the fabrication process to be employed, the costs involved, and the inherent properties of the material other than those covered by this classification system.1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.6 The following precautionary caveat pertains only to the test methods portion, Section 11, of this classification system. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.NOTE 1: Application examples are fully recyclable mattresses and floor coverings. These products can be disassembled into its different parts, by using heat, for refurbishing, repair and full recovery and recycling of the joined materials at the end of the product’s life.1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 Replication is a nondestructive sampling procedure that records and preserves the topography of a metallographically prepared surface as a negative relief on a plastic film (replica). The replica permits the examination and analysis of the metallographically prepared surface on the LM or SEM.4.2 Enhancement procedures for improving replica contrast for microscopic examination are utilized and sometimes necessary (see 8.1).NOTE 1: It is recommended that the purchaser of a field replication service specify that each replicator demonstrate proficiency by providing field prepared replica metallography and direct LM and SEM comparison to laboratory prepared samples of an identical material by grade and service exposure.1.1 This practice covers recognized methods for the preparation and evaluation of cellulose acetate or plastic film replicas which have been obtained from metallographically prepared surfaces. It is designed for the evaluation of replicas to ensure that all significant features of a metallographically prepared surface have been duplicated and preserved on the replica with sufficient detail to permit both LM and SEM examination with optimum resolution and sensitivity.1.2 This practice may be used as a controlling document in commercial situations.1.3 The values stated in SI units are to be regarded as the standard. Inch-pound units given in parentheses are for information only.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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This specification covers mixing water used in the production of hydraulic cement concrete. It defines sources of water and provides requirements and testing frequencies for qualifying individual or combined water sources. Mixing water shall consist of: batch water, ice, water added by truck operator, free moisture on the aggregates, and water introduced in the form of admixtures. Potable and non-potable water is permitted to be used as mixing water in concrete. The following are concrete performance requirements for mixing water: compressive strength and time of set. Density of water shall be tested or monitored with a hydrometer. Optional chemical limits for combined mixing water are given for: chloride, sulfate, alkalis, and total solids.1.1 This specification covers the compositional and performance requirements for water used as mixing water in hydraulic cement concrete. It defines sources of water and provides requirements and testing frequencies for qualifying individual or combined water sources. In any case where the requirements of the purchaser differ from these in this specification, the purchaser’s specification shall govern.1.2 This specification does not purport to cover methods of storage, transportation, or blending of water, or to address the development and maintenance of quality control programs sponsored or managed by the manufacturer.1.3 The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system are not necessarily exact equivalents; therefore, to ensure conformance with the standard, each system shall be used independently of the other, and values from the two systems shall not be combined.1.4 The text of this specification references notes and footnotes that provide explanatory material. These notes and footnotes (excluding those in tables and figures) shall not be considered as requirements of the standard.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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