定价： 515元 / 折扣价： 438 元 加购物车

This guide is intended for use in any laboratory utilizing PCR or RT-PCR to amplify and detect nucleic acid sequences of mycobacteria from a biological preparation and to identify the species of origin.The criteria used for the identification and evaluation of the amplification reactions should be administered by an individual trained in the use of molecular biological and microbiological techniques associated with PCR and MTB.1.1 This guide covers basic considerations, criteria, principles and recommendations that should be helpful when developing, utilizing, or assessing PCR-specific protocols for the amplification and detection or identification of mycobacterial nucleic acids. This guide is not a specific protocol for the detection of specific mycobacteria. It is intended to provide information that will assist the user in obtaining high quality and reliable data. The guide is closely related to and should be used concurrently with the general PCR Guide E 1873.1.2 This guide has been developed for use in any molecular biology or biotechnology laboratory. It may be useful for the detection of mycobacteria in clinical, diagnostic laboratories.1.3 This guide does not cover details of the various methods such as gel electrophoresis that can be utilized to help identify PCR-amplified mycobacterial nucleic acid sequences, and it does not cover details of instrument calibration.1.4 This guide does not cover specific variations of the basic PCR or RT-PCR technology (for example, quantitative PCR, multiplex PCR and in situ PCR), and it does not cover details of instrument calibration.1.5 Warning-Laboratory work involving certain clinical specimens and microorganisms can be hazardous to personnel. Precaution: Biosafety Level 2 facilities are recommended for potentially hazardous work, and Biosafety Level 3 facilities are required for propagating and manipulating Mycobacteria tuberculosis cultures (). Safety guidelines should be adhered to according to NCCLS M29-T2, I17-P and other recommendations ().

定价： 0元 / 折扣价： 0 元

This specification covers polyvinyl chloride and other conforming organic polymer-coated steel chain-link fabric, coated before weaving. Fabric produced from three classes of wire coatings are covered as follows: Class I consists of polymer coatings extruded over zinc-coated, aluminum-coated, or zinc-5 % aluminum-mischmetal alloy-coated, or zinc-5 % aluminum-mischmetal alloy-coated steel wire; Class 2a consists of polymer coating extruded and adhered to zinc-coated, aluminum-coated, or zinc-5 % aluminum-mischmetal alloy-coated steel wire; Class 2b consists of polymer coating fused and adhered to zinc-coated, aluminum-coated, or zinc-5 % aluminum-mischmetal alloy-coated steel wire. Materials shall be tested and the individual grades shall conform to material, weave, mesh size, diamond count, wire size, fabric height, selvage, breaking strength, weight of Zinc, Aluminum, or Zinc-5 % Aluminum-Mischmetal Alloy Coatings, polymer coating thickness, polymer-coated wire, and roll length.1.1 This specification covers polyvinyl chloride and other conforming organic polymer-coated steel chain-link fabric, coated before weaving. Polyvinyl chloride, polyolefin, and other organic polymer coating hereinafter will be designated as polymer coating.1.2 Fabric produced from three classes of wire coatings are covered as follows:1.2.1 Class I consists of polymer coatings extruded over zinc-coated, aluminum-coated, or zinc-5 % aluminum-mischmetal alloy-coated, or zinc-5 % aluminum-mischmetal alloy-coated steel wire.1.2.2 Class 2a consists of polymer coating extruded and adhered to zinc-coated, aluminum-coated, or zinc-5 % aluminum-mischmetal alloy-coated steel wire.1.2.3 Class 2b consists of polymer coating fused and adhered to zinc-coated, aluminum-coated, or zinc-5 % aluminum-mischmetal alloy-coated steel wire.1.3 The values stated in inch-pound units are to be regarded as the standard. The values given in parentheses are provided for information only.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

定价： 918元 / 折扣价： 781 元 加购物车

The intended use of this practice is for chain link fencing of varying heights and designs to be used to enclose a sports field, sport court or recreation facility including the internal fencing required for safety, separation of activities, security, crowd control, access and egress or other requirements.Consideration should be given to fence offset distances from the activity field to provide a safety area for the participants and viewers.This practice is not intended for applications where fencing higher than 12 ft (3660 mm) is desired by the owner.Follow Guide F 1553 format to specify the chain link fence material and installation.Warning Regarding Windscreens and Added Fence Padding—If windscreens or padding are to be installed at the time of fence erection or at a later time, it is advisable to use stronger framework, closer post spacing or back bracing of posts depending on the type of screening material to be used, area of the fence covered and the local wind and weather conditions. Post size and spacing based on wind load can be calculated using the Chain Link Fence Manufacturers Institute’(CLFMI) Guide WL 2445.1.1 This practice is designed to be used for developing the chain-link fence, design, layout and installation for sports and recreation facilities such as sports fields, sports courts, waterfront areas, docks and marinas and other specific facilities.This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

定价： 0元 / 折扣价： 0 元

定价： 515元 / 折扣价： 438 元 加购物车

This specification deals with Grade 80 heat-treated alloy steel chain for applications such as slings, lifting assemblies, and load binding. The alloy steel chain shall be made to a fully-killed fine austenitic grain process. Alloy steel chain may be made by the electric welding or gas welding process. The material shall be heat treated which includes quenching and tempering. Material's composition shall conform to the chemical requirements for carbon, phosphorous, sulfur, nickel, chromium, and molybdenum. The material shall conform to the mechanical property requirements for breaking force and elongation and to the dimensional requirements for the appropriate size chain. All chain shall be tested to at least the proof load.1.1 This specification covers Grade 80 heat-treated alloy steel chain for such applications as slings, lifting assemblies, and load binding. For overhead lifting applications, only alloy chain should be used.1.2 The chain grade is based on the nominal stress in the link at the design breaking force strength. It is calculated by taking the minimum breaking force load and dividing by two times the nominal cross-sectional area of the link.1.3 The values stated in either SI units or in other units are to be regarded separately as standard. The values stated in each system are not necessarily exact equivalents; therefore, to ensure conformance with the standard, each system shall be used independently of the other, and values from the two systems shall not be combined.1.3.1 Metric Units—Grade = 1/10 of the minimum breaking force in kilonewtons divided by two times the nominal cross-sectional area in square millimeters. = (MBF)/(0.005)(π)(d)(d)1.3.2 English Units—Grade = 0.000689 of the minimum breaking force in pounds divided by two times the nominal cross-sectional area in square inches. = (0.000689)(MBF)/(0.5)(π)(d)(d)1.3.3 MBF = minimum breaking force (lb or kN); d = chain diameter (in. or mm).NOTE 1: The above formulas are for round diameter links only. If different cross sections are used, the actual cross section of the link would need to be calculated and used.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 515元 / 折扣价： 438 元 加购物车

5.1 The Intended Use of This Guide is to identify the specific functions and qualities desired of a pickleball fence and offer recommendations on how to achieve them with a chain link fence for different types of pickleball courts.5.2 The Primary Function of a Pickleball Court Fence is to keep pickleball balls in and uninvited traffic out. It also serves as a barrier to prevent players from colliding with fixed objects and spectators. It also prevents players from running onto irregular or slippery surfaces or into dangerous adjacent areas. Fences need to respond to secondary functional requirements, including: spectator viewing, screening the court from wind and visual distractions outside the court, and being as open and welcoming as security conditions permit. In cases where security or dangerous adjacent conditions are an issue, a uniformly high type of enclosure can still be called for, but in most cases, pickleball fences are a variety of heights and may accommodate lights, shade shelters, gates for players and maintenance, and can have completely open (no fence) portions of the perimeter that lead to decks, grandstands, or landscaping1.1 The purpose of this guide is to inform the builder, designer, facility manager, or owner, or a combination thereof, of a pickleball court or facility about the many details and features of pickleball court fence. It focuses on what to consider when designing a pickleball fence, offers some recommendations, and points the user to where they could find additional useful information regarding the design, construction, and maintenance of pickleball courts.1.2 Units—The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units that are provided for information only and are not considered standard.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

This specification covers zinc-coated (galvanized) steel chain-link fence fabric, zinc coated either before or after weaving. The wire shall be woven throughout in the form of approximately uniform square mesh, having parallel sides and horizontal and vertical diagonals of approximately uniform dimensions. The top and bottom of the fabric shall be knuckled or twisted. A typical diamond count for each standard height is given. Chain-link fabric shall conform to the requirements specified for (1) mesh size, (2) wire size, (3) fabric height, (4) selvage, (5) zinc coating weight (Class 1or Class 2), and (6) breaking strength. The standard length of rolls and sampling requirements for testing are specified as well.1.1 This specification covers zinc-coated steel chain-link fence fabric, zinc coated either before or after weaving.1.2 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units that are provided for information only and are not considered standard.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

This specification covers carbon steel chain for such applications as railroad cars, construction, industrial uses, load binding, and general purposes other than overhead lifting. The three classes of carbon steel chains covered here include Grade 30 (proof coil chain), Grade 43 (high test chain), and Grade 70 (transport chain). The dimensional requirements of the chain with respect to the appropriate grade and size are presented. The mechanical testing requirements for the steel includes, proof testing, breaking force testing, and elongation testing. One test for breaking strength and elongation shall be made from each lot. The elongation and breaking force tests may be performed at the same time on the same test specimen.1.1 This specification covers carbon steel chain for such applications as railroad cars, construction, industrial uses, load binding, and general purposes other than overhead lifting.NOTE 1: This specification does not cover carbon steel chain for sprocket applications.1.2 Three classes of carbon steel chain are covered:1.2.1 Grade 30—Proof coil chain.1.2.2 Grade 43—High test chain.1.2.3 Grade 70—Transport chain.1.3 The chain grade is based on the nominal stress in the link at the design breaking force strength. It is calculated by taking the minimum breaking force load and dividing by two times the nominal cross-sectional area of the link.1.4 The values stated in either SI units or in other units are to be regarded separately as standard. The values stated in each system are not necessarily exact equivalents; therefore, to ensure conformance with the standard, each system shall be used independently of the other, and values from the two systems shall not be combined.1.4.1 Metric Units—Grade = 1/10 of the minimum breaking force in kilonewtons divided by two times the nominal cross-sectional area in square millimeters. = (MBF)/(0.005)(π)(d)(d)1.4.2 English Units—Grade = 0.000689 of the minimum breaking force in pounds divided by two times the nominal cross-sectional area in square inches. = (0.000689)(MBF)/(0.5)(π)(d)(d)1.4.3 MBF = minimum breaking force (lb or kN); d = chain diameter (in. or mm).NOTE 2: The above formulas are for round diameter links only. If different cross sections are used, the actual cross section of the link would need to be calculated and used.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 515元 / 折扣价： 438 元 加购物车

定价： 515元 / 折扣价： 438 元 加购物车

定价： 590元 / 折扣价： 502 元 加购物车

定价： 590元 / 折扣价： 502 元 加购物车

This specification prescribes the minimum requirements for the design, performance, testing, and certification of protective garments and protective devices that, when worn by operators of power chain saws, shall provide cut resistance protection to their legs, and thereby reducing leg injuries caused by close contact with the running chain saw. The protective garments and devices shall be constructed with suitable fit and flexibility so as to provide sufficient leg comfort and normal maneuverability to perform intended tasks, while maintaining satisfactory functionality and efficacy for the duration of its regular use throughout seasonal climatic variations. Completed garments shall be inspected and certified as specified, and maintained in accordance with the manufacturer's instructions.1.1 This specification specifies minimum requirements for the design, performance, testing, and certification of protective clothing and protective devices designed to provide cut resistance protection to the legs of operators of power chain saws.1.2 The objective of this specification is to prescribe fit, function, and performance criteria for protective clothing and protective devices that, when worn by chain saw operators, are intended to reduce the severity of leg injuries caused by contact with a running power saw chain.1.3 This standard is not intended to serve as a detailed manufacturing or purchasing specification, but can be referenced in purchase contracts to ensure that minimum performance requirements are met.1.4 Controlled laboratory tests used to determine compliance with the performance requirements of this standard shall not be deemed as establishing performance levels for all situations to which chain saw operators may be exposed.1.5 Mandatory requirements are indicated by the use of “shall”; recommendations and advisory information is indicated by use of “should.”1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

定价： 0元 / 折扣价： 0 元 加购物车