6.1 This guide can be used to quantitatively assess the intensity of specific attributes of hair odors resulting from hair-care products.6.2 This guide may be utilized for product development, research guidance, and quality control.6.3 These are suggested procedures and are not meant to exclude alternate procedures that may effectively provide the same or similar results.1.1 This guide covers standardized procedures for the quantitative sensory assessment of fragrance/odor intensity or attribute intensity of fragrances in hair-care products through all stages of use (point of purchase, lather, in use, wet hair after rinse, and dry hair) under laboratory conditions with trained assessors.1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This test method provides a relatively simple and reliable microscopical means of measuring the phase abundance of portland cement clinker (Note 1). Microscopical point counting provides a direct measure of the clinker phase composition in contrast to the calculated Bogue phase composition (Note 2).NOTE 1: This test method utilizes a reflected light microscope. Related methods such as transmitted light microscopy, scanning electron microscopy, and automated imaging techniques may also be used for clinker analysis but are not presently included in this test method.NOTE 2: This test method allows direct determination of the proportion of each individual phase in portland cement clinker. This test method is intended to provide an alternative to the indirect estimation of phase proportion using the equations in Specification C150/C150M (footnote C in Table 1 and footnote B in Table 2).5.2 This test method assumes the operator is qualified to operate a reflected light microscope and the required accessories, is able to correctly prepare polished sections and use necessary etchants, and is able to correctly identify the constituent phases.5.3 This test method may be used as part of a quality control program in cement manufacturing as well as a troubleshooting tool. Microscopic characterization of clinker phases may also aid in correlating cement properties and cement performance in concrete, to the extent that properties and performance are a function of phase composition.1.1 This test method covers a systematic procedure for measuring the percentage volume of the phases in portland cement clinker by microscopy.1.2 The values stated in SI units are to be regarded as the standard.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 Overview of Measurement System—Relative intensity measurements made by widefield epifluorescence microscopy are used as part of cell-based assays to quantify attributes such as the abundance of probe molecules (see ASTM F2997), fluorescently labeled antibodies, or fluorescence protein reporter molecules. The general procedure for quantifying relative intensities is to acquire digital images, then to perform image analysis to segment objects and compute intensities. The raw digital images acquired by epifluorescence microscopy are not typically amenable to relative intensity quantification because of the factors listed in 4.2. This guide offers a checklist of potential sources of bias that are often present in fluorescent microscopy images and suggests approaches for storing and normalizing raw image data to assure that computations are unbiased.5.2 Areas of Application—Widefield fluorescence microscopy is frequently used to measure the location and abundance of fluorescent probe molecules within or between cells. In instances where RIM comparisons are made between a region of interest (ROI) and another ROI, accurate normalization procedures are essential to the measurement process to minimize biased results. Example use cases where this guidance document may be applicable include:5.2.1 Characterization of cell cycle distribution by quantifying the abundance of DNA in individual cells (1).75.2.2 Measuring the area of positively stained mineralized deposits in cell cultures (ASTM F2997).5.2.3 Quantifying the spread area of fixed cells (ASTM F2998).5.2.4 Determining DNA damage in eukaryotic cells using the comet assay (ASTM E2186).5.2.5 The quantitation of a secondary fluorescent marker that provides information related to the genotype, phenotype, biological activity, or biochemical features of a colony or cell (ASTM F2944).1.1 This guidance document has been developed to facilitate the collection of microscopy images with an epifluorescence microscope that allow quantitative fluorescence measurements to be extracted from the images. The document is tailored to cell biologists that often use fluorescent staining techniques to visualize components of a cell-based experimental system. Quantitative comparison of the intensity data available in these images is only possible if the images are quantitative based on sound experimental design and appropriate operation of the digital array detector, such as a charge coupled device (CCD) or a scientific complementary metal oxide semiconductor (sCMOS) or similar camera. Issues involving the array detector and controller software settings including collection of dark count images to estimate the offset, flat-field correction, background correction, benchmarking of the excitation lamp and the fluorescent collection optics are considered.1.2 This document is developed around epifluorescence microscopy, but it is likely that many of the issues discussed here are applicable to quantitative imaging in other fluorescence microscopy systems such as fluorescence confocal microscopy. This guide is developed around single-color fluorescence microscopy imaging or multi-color imaging where the measured fluorescence is spectrally well separated.1.3 Fluorescence intensity is a relative measurement and does not in itself have an associated SI unit. This document does discuss metrology issues related to relative measurements and experimental designs that may be required to ensure quantitative fluorescence measurements are comparable after changing microscope, sample, and lamp configurations.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 The design of this test eliminates any loss of viable organisms through wash off, thus making it possible to produce statistically valid data using many fewer test carriers than needed for methods based on simple MPN estimates.5.2 The stringency in the test is provided by the use of a soil load, the microtopography of the brushed stainless steel carrier surface, and the smaller ratio of test substance to surface area typical for many disinfectant applications. Thus, the test substance being assessed is presented with a reasonable challenge while allowing for efficient recovery of the test organisms from the inoculated carriers. The metal disks in the basic test are also compatible with a wide variety of actives.5.3 The design of the carriers makes it possible to place onto each a precisely measured volume of the test organism (10 μL) as well as the control fluid or test substance (50 μL).5.4 The inoculum is placed at the center of each disk whereas the volumes of the test substance covers nearly the entire disk surface, thus virtually eliminating the risk of any organisms remaining unexposed.5.5 In all tests, other than those against viruses, the addition of 10 mL of an eluent/diluent gives a 1:200 dilution of the test substance immediately at the end of the contact time. While this step in itself may be sufficient to arrest the microbicidal activity of most actives, the test protocol permits the addition of a specific neutralizer to the eluent/diluent, if required. Except for viruses, the membrane filtration step also allows processing of the entire eluate from the test carriers and, therefore, the capture and subsequent detection of even low numbers of viable organisms that may be present. Subsequent rinsing of the membrane filters with saline also reduces the risk of carrying any inhibitory residues over to the recovery medium. Validation of the process of neutralization of the test substance is required by challenge with low numbers of the test organism.5.6 In tests against viruses, addition of 1 mL of buffer at the end of the contact time achieves a 1:20 dilution of the test substance while keeping the volume of the eluate reasonably small to allow for the titration of most or all of the eluate in cell cultures. Confirmation of neutralization of the test substance is required by challenge of a residual disinfection load with low numbers of infective units of the test virus. Since the virus assay system is indirect, an additional step is required to demonstrate that prior exposure of the appropriate cell line to any residual disinfectant or disinfectant/neutralizer mixture does not interfere with the detection of a low level of virus challenge (See Appendix).NOTE 1: In 5.5 and 5.6, volumes of 10 mL and 1 mL are recommended instead of 9.95 mL and 950 μL, respectively, for ease of dispensing the eluent.5.7 The soil load in this test is a mixture of three types of proteins (high molecular weight proteins, low molecular weight peptides, and mucous material) designed to represent body secretions, excretions, or other extraneous substances that microbicidal chemicals may encounter under field conditions. It is suitable for working with all types of test organisms included here. The components of the soil load are readily available and subject to much less variability than animal sera.5.8 If distilled water or other diluent is not to be specified on the product label, the diluent for the test substance is assumed to be tap water. Since the quality of tap water varies considerably both geographically and temporally, this test method incorporates the use of water with a specified and documented level of hardness to prepare use-dilutions of test substance that require dilution in water before use. While water with a hardness of at least 300 ppm as CaCO3 is recommended consult local regulations regarding use of hard water prior to testing.5.9 The Annex contains a list of those organisms that are often used in assessing the microbicidal activities of disinfectants for use on environmental surfaces or medical devices. Culture conditions for each organism are also included in the Annex. Depending on the label claim(s) desired and the requirements of the target regulatory agency, one or more of the organisms listed may be selected for the testing. If organisms other than those listed are to be used (for example, in the dairy or brewing industries), a clear justification must be provided and details of the culture media and growth conditions must be validated and clearly specified in test reports.1.1 This test method is designed to evaluate the ability of test substances to inactivate vegetative bacteria, viruses, fungi, mycobacteria, and bacterial spores (1-7) on disk carriers of brushed stainless steel that represent hard, nonporous environmental surfaces and medical devices. It is also designed to have survivors that can be compared to the mean of no less than three control carriers to determine if the performance standard has been met. For proper statistical evaluation of the results, the number of viable organisms in the test inoculum should be sufficiently high to take into account both the performance standard and the experimental variations in the results.1.2 The test protocol does not include any wiping or rubbing action. It is, therefore, not designed for testing wipes.1.3 This test method should be performed by persons with training in microbiology in facilities designed and equipped for work with infectious agents at the appropriate biosafety level (8).1.4 It is the responsibility of the investigator to determine whether Good Laboratory Practice Regulations (GLPs) are required and to follow them where appropriate (40 CFR, Part 160 for EPA submissions and 21 CFR, Part 58 for FDA submissions).1.5 In this test method, SI units are used for all applications, except for distance in which case inches are used and metric units follow.1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This test is intended to assess the mechanical integrity, failure modes, and practical adhesion strength of a specific hard ceramic coating on a given metal or ceramic substrate. The test method does not measure the fundamental “adhesion strength” of the bond between the coating and the substrate. Rather, the test method gives a quantitative engineering measurement of the practical (extrinsic) adhesion strength and damage resistance of the coating-substrate system as a function of applied normal force. The adhesion strength and damage modes depend on the complex interaction of the coating-substrate properties (hardness, fracture strength, modulus of elasticity, damage mechanisms, microstructure, flaw population, surface roughness, and so forth) and the test parameters (stylus properties and geometry, loading rate, displacement rate, and so forth).5.2 The test method as described herein is not appropriate for polymer coatings, ductile metal coatings, very thin (<0.1 μm) ceramic coatings, or very thick (>30 μm) ceramic coatings.NOTE 2: Under narrow circumstances, the test may be used for ceramic coatings on polymer substrates with due consideration of the differences in elastic modulus, ductility, and strength between the two types of materials. Commonly, the low comparative modulus of the polymer substrate means that the ceramic coating will generally tend to fail in bending (through-thickness adhesive failure) before cohesive failure in the coating itself.5.3 The quantitative coating adhesion scratch test is a simple, practical, and rapid test. However, reliable and reproducible test results require careful control of the test system configuration and testing parameters, detailed analysis of the coating damage features, and appropriate characterization of the properties and morphology of the coating and the substrate of the test specimens.5.4 The coating adhesion test has direct application across the full range of coating development, engineering, and production efforts. Measurements of the damage mechanisms in a coating as a function of applied normal forces are useful to understand material-process-property relations; quantify and qualify the mechanical response of coating-substrate systems; assess coating durability; measure production quality; and support failure analysis.5.5 This test method is applicable to a wide range of hard ceramic coating compositions (carbides, nitrides, oxides, diamond, and diamond-like carbon) applied by physical vapor deposition, chemical vapor deposition, and direct oxidation methods to metal and ceramic substrates.5.6 Ceramic coatings can be crystalline or amorphous, but commonly have high relative density with limited porosity (<5 %). Porous coatings can be tested, but the effects of porosity on the damage mechanisms in the coating must be carefully considered.5.7 The test method, as defined with the 200 μm radius Rockwell diamond stylus, is commonly used for ceramic coating thicknesses in the range of 0.10 to 30 μm. Thinner coatings may require a smaller diameter stylus and lower normal forces for reliable results. Thicker coatings may require larger diameter stylus and higher normal forces. Any variations in stylus size and geometry and designated normal force ranges shall be reported.5.8 Specimens commonly have a flat planar surface for testing, but cylinder geometries can also be tested if they are properly fixtured and aligned and the scratch direction is along the long axis of the specimen. The physical size of the test specimen is determined primarily by the capabilities and limits of the test equipment stage and fixturing.5.9 The test is commonly conducted under unlubricated conditions and at room temperature. However, it is feasible and possible to modify the test equipment and test conditions to conduct the test with lubrication or at elevated temperatures.5.10 Coated specimens can be tested after high temperature, oxidative, or corrosive exposure to assess the retained properties and durability (short-term and long-term) of the coating. Any specimen conditioning or environmental exposure shall be fully documented in the test report, describing in detail the exposure conditions (temperature, atmosphere, pressures, chemistry, humidity, and so forth), the length of time, and resulting changes in coating morphology, composition, and microstructure.1.1 This test method covers the determination of the practical adhesion strength and mechanical failure modes of hard (Vickers Hardness HV = 5 GPa or higher), thin (≤30 μm) ceramic coatings on metal and ceramic substrates at ambient temperatures. These ceramic coatings are commonly used for wear/abrasion resistance, oxidation protection, and functional (optical, magnetic, electronic, biological) performance improvement.1.2 In the test method, a diamond stylus of defined geometry (Rockwell C, a conical diamond indenter with an included angle of 120° and a spherical tip radius of 200 μm) is drawn across the flat surface of a coated test specimen at a constant speed and a defined normal force (constant or progressively increasing) for a defined distance. The damage along the scratch track is microscopically assessed as a function of the applied force. Specific levels of progressive damage are associated with increasing normal stylus forces. The force level(s) which produce a specific type/level of damage in the coating are defined as a critical scratch load(s). The test method also describes the use of tangential force and acoustic emission signals as secondary test data to identify different coating damage levels.1.3 Applicability to Coatings—This test method is applicable to a wide range of hard ceramic coating compositions: carbides, nitrides, oxides, diamond, and diamond-like carbon on ceramic and metal substrates. The test method, as defined with the 200 μm radius diamond stylus, is commonly used for coating thicknesses in the range of 0.1 to 30 μm. Test specimens generally have a planar surface for testing, but cylinder geometries can also be tested with an appropriate fixture.1.4 Principal Limitations: 1.4.1 The test method does not measure the fundamental adhesion strength of the bond between the coating and the substrate. Rather, the test method gives an engineering measurement of the practical (extrinsic) adhesion strength of a coating-substrate system, which depends on the complex interaction of the test parameters (stylus properties and geometry, loading rate, displacement rate, and so forth) and the coating-substrate properties (hardness, fracture strength, modulus of elasticity, damage mechanisms, microstructure, flaw population, surface roughness, and so forth).1.4.2 The defined test method is not directly applicable to metal or polymeric coatings which fail in a ductile, plastic manner, because plastic deformation mechanisms are very different than the brittle damage modes and features observed in hard ceramic coatings. The test method may be applicable to hard metal coatings which fail in a brittle mode with appropriate changes in test parameters and damage analysis procedures and criteria.1.4.3 The test method, as defined with the Rockwell C diamond stylus and specific normal force and rate parameters, is not recommended for very thin (<0.1 μm) or thicker coatings (>30 μm). Such coatings may require different stylus geometries, loading rates, and ranges of applied normal force for usable, accurate, repeatable results.1.4.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. Test data values in SI units (newtons (N) for force and millimetres (mm) for displacement) are to be considered as standard and are in accordance with IEEE/ASTM SI 10.1.5 Organization—The test method is organized into the following sections:  Section 1 Purpose and Description 1.1 Applicability 1.3 Principal Limitations 1.4 Organization 1.5Referenced Documents 2 ASTM Standards 2.1 Other Standards and References 2.2Terminology 3Summary of Test Method 4 5Test Methodology and Experimental Control 6 Test Overview 6.1 Test Modes 6.2 Primary and Supplementary Measurements 6.3 Critical Scratch Load Damage Criteria and Scratch Atlas 6.4 Experimental Factors and Variables 6.5Interferences 7 Material and Specimen Related 7.2 Test Method Related 7.3Apparatus 8 General Description 8.1 Stylus and Stylus Mounting 8.2 Mechanical Stage and Displacement Control 8.3 Test Frame and Force Application System 8.4 Force and Displacement Sensors 8.5 Optical Analysis and Measurement 8.6 Data Acquisition and Recording 8.7 Acoustic Emission (Optional) 8.8 Coating Adhesion Reference Specimens (Optional) 8.9 Coating Surface Profilometry (Optional) 8.10 Data Analysis and Output Software (Optional) 8.11Test Specimens 9 Specimen Requirements 9.1 Specimen Characterization 9.2 Specimen Size 9.3 Specimen Flatness and Level 9.4 Polishing (Optional) 9.5 Specimen Exposure Conditioning (Optional) 9.6 Specimen Cleaning 9.7 Specimen Handling and Storage 9.8Calibration 10 System Calibration 10.1 Reference Specimens 10.2Test Procedure 11 Calibration 11.1 Test Mode Selection 11.2 Test Planning 11.3 Stylus Inspection and Cleaning 11.4 Environmental Conditions 11.5 System Setup and Check 11.6 Test Specimen Mounting 11.7 Conducting the Test 11.8 Specimen Count 11.9 Invalid and Censored Data 11.10 Scratch Damage Assessment 11.11Calculations 12Report 13 Test Identification 13.2 Specimen Information 13.3 Test Equipment and Procedure Information 13.4 Test Data and Statistics 13.5Precision and Bias 14Keywords 15Rockwell Diamond Indenter Specifications Annex A1Alignment and Calibration Annex A2Repeatability and Reproducibility Studies Annex A3Coating Damage Criteria and Scratch Atlas Appendix X1Experimental Variables in Scratch Adhesion Testing Appendix X2Bibliography  1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 This guide is intended to assist those writing or revising compositional specifications, sampling practices, and test methods for ferrous and non-ferrous metals, ores, and related materials. It is directed toward those areas that must be addressed to properly coordinate compositional specification, sampling practice, and test methods. Its use will help ensure that compositional requirements are clearly defined and that sampling practices and test methods are available to meet product specifications.4.2 This guide does not attempt to define which elements should be controlled, where samples should be taken, or how they should be analyzed. These items are addressed in standards such as Specification A276, Test Methods and Practices A751, Test Method E34, Practice E255, Test Method E342, and Test Methods E350.4.3 A primary purpose for ASTM sampling practices and test methods is to provide widely-accepted and tested methodology for use in meeting ASTM product specifications. Although it is recognized that individual laboratories are free to use other methods, the availability of ASTM approved methodology is essential for referee purposes and to demonstrate that properly equipped laboratories can make the required measurements.4.4 Sampling practices and test methods to be recommended for use in testing a given product are most easily selected cooperatively by the specification-writing and the methods-writing committees that have jurisdiction over the product. When existing sampling or test methods do not meet the needs of the new product specification standard, the specification-writing committee should request that the methods-writing committee develop the required standards. ASTM Committee E01 is responsible for test methods and practices covering the sampling and analysis of most metals, ores, and related materials.1.1 This guide covers procedures for specifying compositional requirements and identifying appropriate sampling and quantitative analysis test methods to be referenced in product specification standards for metals, ores, and related materials. It is not intended to replace or conflict with either individual product specifications or standards covering broad classifications of products such as Test Methods and Practices A751.1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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