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5.1 The durability of antimicrobial agents applied to textiles is an important attribute for many of the available technologies on the market. Antimicrobial agents that claim durability are typically fixed ionically, covalently or physically, or both, to a textile surface and are expected to retain their antimicrobial functionality after 5, 25 or 50 washes.5.2 Textile wash standards do exist that measure features as diverse as colorfastness or softener retention, pilling, or even the appearance of the decorative coatings of a zipper; however, no wash method exists that is specific for measuring the durability of an antimicrobial agent applied directly into or onto a textile surface.5.3 Current wash standards have been written to either closely simulate (AATCC TM135) or accelerate (AATCC TM61) the laundering conditions that would be experienced during normal home laundering. While shown to be effective when testing physical properties of textiles, these methods introduce variables to the washing protocol that can directly affect the final antimicrobial properties of a fabric. For example, many wash protocols add bleach or softeners which can build up over time and may introduce false positive results in industry standard microbiological tests. Conversely, powdered detergents if not completely rinsed after each wash can leave residual surfactants that can build up over time but are generally removed during wear. These residual detergents can potentially coat an antimicrobial surface and provide false negative results.5.4 Very specific parameters are identified within this practice to closely replicate home launderings as identified and studied in previous wash protocols (AATCC TM61) and accepted within the textile industry. This practice uses detergents and washing conditions which limit potential cross contamination of samples during washing and unrealistic deposition of residual detergents on the test fabric. These conditions increase the reproducibility and reliability of subsequent microbiological test methods.5.5 This practice allows for the simple washing of textile fabrics for the subsequent antimicrobial testing. Any industry accepted antimicrobial test standard could be used following this washing protocol.5.6 This practice is appropriate for porous materials such as textiles or any porous, soft substrate that is intended to withstand multiple home washes. This practice is intended to measure the durable antibacterial properties of such materials. In most instances, further studies will be required to support and substantiate actual claims being made for the performance of treated materials in practice or as part of a regulatory process.5.7 This standard practice has been shown to be effective at measuring the durability of polymer based antimicrobial agents to home laundering conditions. Particle based or other antimicrobial agents may require modifications of the current methodology to simulate laundering conditions in practice. The exact correlation between expressed laundry care instructions on the antimicrobial treated article and the exposure conditions identified in the standard practice must be determined separately for every antimicrobial active.1.1 To determine the durability of standard antibacterial treatments on textile products such as apparel, piece goods, household articles, hereinafter referred to as “textile” or “textile products” to multiple home launderings.1.2 This practice subjects textile products treated with antimicrobial agents to multiple simulated and accelerated home launderings under defined parameters such that reproducible and reliable antimicrobial analysis can be performed using standard industry accepted protocols.1.3 For some antimicrobial agents, the durability of antibacterial properties resulting from exposure to detergent solution and abrasive action of multiple home launderings has been shown to be approximated by one 45-minute laundering cycle. The exact correlation between expressed laundry care instructions and exposure conditions identified in the practice should be determined separately for every antimicrobial agent.1.4 The subsequent microbiological methods shall be performed by individuals experienced and adept in microbiological procedures and in facilities suitable for the handling of the microorganisms under test.1.5 This standard may involve hazardous materials, operation, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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3.1 This practice may be used to determine non-protein or non-nitrogen containing organic matter in leather which is not extractable with water or hexane. Examples would be vegetable tannins and acrylic lubricants.1.1 This practice covers the determination of the combined tannin and nonextractable organic resins and the degree of tannage of all types of vegetable-tanned leather and leather with organic retannages. This practice does not apply to wet blue.1.2 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This guide is to be used by anyone developing cleaning requirements for specifications for manufacturing, maintenance, or overhaul. This guide has been designed to be application specific for each cleaning task and to assure the design engineer that the process selected by the industrial or manufacturing engineer will be compatible with both the part material and the subsequent process(es). This guide allows the industrial or manufacturing engineer to customize the selection of the cleaning product based on the materials of the part being cleaned; the cleanliness required for the subsequent process(es); and the environmental, cost, and health and safety concerns.1.1 This guide is intended to assist design engineers, manufacturing/industrial engineers, and production managers in selecting the best fit cleaning agent and process. This guide takes into account environmental pollution prevention factors in a selection process.1.2 This guide is not to be considered as a database of acceptable materials. It will guide the engineers and managers through the cleaning material selection process, calling for engineers to customize their selection based on the cleaning requirements for the cleaning tasks at hand. If a part can be cleaned, and kept clean, it can be cycled through several process steps that have cleaning requirements. This eliminates extra cleaning process steps during the total process. A total life cycle cost analysis or performance/cost of ownership study is recommended to compare the methods available.1.3 This guide is for general industry manufacturing, equipment maintenance and remanufacturing operations, and to some extent precision cleaning of mechanical parts and assemblies. It is not intended to be used for optical, medical, or electronics applications, nor is it intended for dry-cleaning or super-critical fluid cleaning.1.4 The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system are not necessarily exact equivalents; therefore, to ensure conformance with the standard, each system shall be used independently of the other and values from the two systems shall not be combined.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This practice should be used to determine if a fungal control agent is effective to preserve pigment suspensions, dye solutions, pulp slurries, starch solutions, polymers, sizing agents, latex emulsions, and other specific aqueous-based materials used in the paper industry. Separate evaluations should be made on a representative type for each specific class of product to be preserved.NOTE 1: Control of bacterial spoilage of similar products can be evaluated by Test Method E723.NOTE 2: Slimicides for control of fungal or bacterial slime can be evaluated by Test Method E1839.1.1 This laboratory practice is used to determine the efficacy of a fungal control agent to prevent spoilage of in-process aqueous-based products used in the paper industry.1.2 For information on bacterial control agents, see Test Method E723.1.3 It is the responsibility of the investigator to determine whether good laboratory practices (GLP) are required and to follow them when appropriate (see 40 CFR 160).1.4 A knowledge of microbiological techniques is required for these procedures.1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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ASTM E2407-04(2015) Standard Test Method for Effectiveness of Defoaming Agents (Withdrawn 2024) Withdrawn, No replacement 发布日期 :  1970-01-01 实施日期 : 

5.1 This test is designed to determine the ability of a material to reduce or eliminate undesirable foam that can be generated during the mixing and application of agricultural chemicals in dilute aqueous mixtures.5.2 This method is useful for testing liquid defoaming agents, such as “silicone emulsion” or “organic” type defoaming agents. In principle, it might also be used to test dry antifoam agents with minor modifications to the procedure.5.3 This method could be employed to determine the relative effectiveness of one defoaming agent versus another. It is left to the user of this method to interpret the results with respect to the actual field use of the defoaming agents tested.5.4 This method assumes that the defoaming agent being tested has sufficient durability to maintain its activity for the duration of the test.5.5 Sodium lauryl ether sulfate as the foaming agent is described in Section 7, Reagents. If desired the user of this test method may select an alternative suitable foaming agent.1.1 This test method describes a qualitative method for the evaluation of liquid defoaming agents used to control undesirable foam in dilute, aqueous surfactant solutions. This laboratory test method may be applied to aqueous systems containing additional components, such as agricultural chemicals. This method is described using SI units1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.3 This standard method does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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This test method covers the determination of the efficiency of ordinary commercial wetting agents using the skein test. It is applicable under limited and controlled conditions, but does not necessarily yield information correlating with specific end uses. The apparatus is comprised of a hook of standard weight depending on the concentration of the wetting agents and an anchor which shall be a flat, cylindrical, lead slug of specific dimensions. The reagents shall consist of water, acid and base test solutions, and wetting agent. The average of at least four determinations of the sinking time for each concentration of wetting agent shall be obtained and presented in plots with logarithmic coordinates. Standard deviation based on six replicate determinations shall also be calculated for precision.1.1 This test method2,3 covers the determination of the efficiency of ordinary commercial wetting agents as defined in Terminology D459. This test method is applicable under limited and controlled conditions, but does not necessarily yield information correlating with specific end uses.1.2 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units that are provided for information only and are not considered standard.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 This practice specifies an in-vivo measurement of CWA decontamination on the skin.4.2 CWA skin decontaminants will have different modes of action including absorption, adsorption, removal, chemical neutralization or some combination of the above. There is, therefore, no single representative in-vitro method for validation of decontamination efficacy of products for skin decontamination. For example, measuring the presence of a radiolabelled chemical warfare agent after chemical neutralization, may give a false positive results. It has been shown that if the agent has been chemically neutralized, the radiolabel may still be present in a non-toxic molecule. In addition, some chemical neutralization methods may break down the original agent, but the breakdown product is highly toxic. In the case of VX, hydrolysis produces a highly toxic product, EA2192 (S-(2-diisopropylaminoethyl) methylphosphonothioic acid (8).4.3 This standard practice is of significance in that efficacy is thoroughly evaluated to the extent possible to represent use on human skin. In-vivo studies have demonstrated that simple chemical monitoring for disappearance of the chemical agent may not be sufficient to measure decontamination and neutralization effectiveness. A standard practice is needed for determining actual decontamination and neutralization by measuring the decrease in mortality or lesion size caused by the agent.1.1 This practice establishes an in-vivo method for assessing the comparative efficacy of products used for the decontamination of chemical warfare agents (CWAs) on the skin.1.2 This practice provides a quantitative efficacy comparison of different skin decontamination products.1.3 To minimize the number of animals used, this in-vivo practice should be performed only after rigorous in-vitro studies of the candidate decontaminant, which can show the implied claims including chemical neutralization, decontamination studies on surfaces and appropriate testing such as cytotoxicity.1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.5 This standard does not purport to address all of the safety concerns, if any, associated with the use of decontamination products or CWAs. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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ASTM D4552/D4552M-20 Standard Classification for Hot-Mix Recycling Agents Active 发布日期 :  1970-01-01 实施日期 : 

3.1 Recycling of deteriorated asphalt pavements is being used with increasing frequency for its economy and benefit of conserving raw materials. The objective of recycling is to reuse the two ingredients of asphalt concrete-aggregate and asphalt binder and to restore the desired properties to the mixture. Recycling is carried out hot or cold, depending on the condition of the deteriorated pavement, construction procedure, availability of equipment, and cost. This classification is for classifying recycling agents to be used in recycled asphalt mixtures.NOTE 3: The quality of the results produced by this standard are dependent on the competence of the personnel performing the procedure and the capability, calibration, and maintenance of the equipment used. Agencies that meet the criteria of Specification D3666 are generally considered capable of competent and objective testing, sampling, inspection, etc. Users of this standard are cautioned that compliance with Specification D3666 alone does not completely ensure reliable results. Reliable results depend on many factors; following the suggestions of Specification D3666 or some similar acceptable guideline provides a means of evaluating and controlling some of those factors.1.1 This standard covers a standardized method whereby recycling agents to be used in hot recycling of asphalt concrete can be classified. The recycling agents are classified by viscosity in mm2/s measured at 60 °C [140 °F]. This classification does not apply to emulsified recycling agents.1.2 This standard does not address the performance of asphalt binder blends with recycling agents, or that of hot-mix asphalt mixture containing recycling agents. Adherence of a recycling agent to this specification does not necessarily relate to the performance of asphalt binders and mixtures containing the recycling agents.NOTE 1: The impact of recycling agents has been evaluated by extracting the asphalt binder from the pavement to be recycled, and combining with the recycling agent to meet the appropriate grade within Specification D946/D946M or Table 1, 2, or 3 of Specification D3381/D3381M. More recently, some specifications have referred to performance grading as described in Specification D6373 or D8239.NOTE 2: Efforts are underway to utilize performance-based testing of the asphalt mixture containing recycling agents as a measure of compliance.1.3 The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system may not be exact equivalents; therefore, each system shall be used independently of the other. Combining values from the two systems may result in nonconformance with the standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 Substrate–bonded, antimicrobial agents are not typically free to diffuse into their environment under normal conditions of use. This test method ensures good contact between the bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the test specimen in a challenge suspension during the test period.5.2 The metabolic state of the challenge species can directly affect measurements of the effectiveness of particular antimicrobial agents or concentrations of agents. The susceptibility of the species to particular biocides could be altered depending on its life stage (cycle). One-hour contact time in a buffer solution allows for metabolic stasis in the population. This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism.5.3 Leaching of an antimicrobial is dependent upon the test conditions being utilized and the ultimate end use of the product. Additional testing may be required to determine if a compound is substrate-bound in all conditions or during the end use of the product.5.4 This test method cannot determine if a compound is leaching into solution or is immobilized on the substrate. This test method is only intended to determine efficacy as described in subsequent portions of the method.5.5 The test is suitable for evaluating stressed or modified specimens, when accompanied by adequate controls.NOTE 1: Stresses may include laundry, wear and abrasion, radiation and steam sterilization, UV exposure, solvent manipulation, temperature susceptibility, or similar physical or chemical manipulation.1.1 This test method is designed to evaluate the antimicrobial activity of antimicrobial-treated specimens under dynamic contact conditions. This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials. These difficulties include ensuring contact of inoculum to treated surface (as in AATCC TM100), flexibility of retrieval at different contact times, use of inappropriately applied static conditions (as in AATCC TM147), sensitivity, and reproducibility.1.2 This test method allows for the ability to evaluate many different types of treated substrates and a wide range of microorganisms. Treated substrates used in this test method can be subjected to a wide variety of physical/chemical stresses or manipulations and allows for the versatility of testing the effect of contamination due to such things as hard water, proteins, blood, serum, various chemicals, and other contaminants.1.3 Surface antimicrobial activity is determined by comparing results from the test sample to controls run simultaneously.1.4 This test method may not be appropriate for all types of antimicrobial-treated articles or antimicrobial agents. The proper test methodology should be determined based on antimicrobial mode of action and end-use expectations (Guide E2922)1.5 Proper neutralization of all antimicrobials must be confirmed using Test Methods E1054.1.6 This test method should be performed only by those trained in microbiological techniques.1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.8 This standard may involve hazardous materials, operations and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This in vivo procedure is designed to test the ability of hygienic handwash and handrub agents to reduce levels of selected infectious viruses from experimentally contaminated fingerpads of adults. Since the two thumbpads and all eight fingerpads can be contaminated with virus and used in a given test, it allows for the incorporation of a wet inoculum input control, dried virus recovery control, and up to three replicates to assess the virus-eliminating efficiency of a test or control agent, or a vehicle material. No more than 100 μL of the virus suspension are required to complete one test.5.2 This test method is designed to be performed by a trained individual, who is responsible for choosing the appropriate host system for the test virus and applying the techniques necessary for propagation and maintenance of host and test virus. For a reference text, refer to Lennette et al (12).5.3 Whereas the method described here relates to testing with viruses of human origin, it can be readily adapted to work with animal pathogenic viruses as well as bacteriophages. Standard methods for working with bacteria (Test Method E2276) and fungi (Test Method E2613) are also available.5.4 Infectious microorganisms left on hands after washing can be reduced further by drying the washed hands with paper, cloth, or warm air (13). A step for the drying of fingerpads after exposure to the control or test product, therefore, has not been included to avoid virus removal by the drying process itself.5.5 This test method is not meant for use with surgical hand scrubs or preoperative skin preps.5.6 The level of viable virus in the dried inocula the control fingerpads should not be less than 104 infectious units which would permit the detection of up to a 4 log10 reduction in the infectivity titer of the virus by the test product under the conditions of this test method.1.1 Human skin is not known to carry viruses as a part of its resident microbiota, with the notable exception of papilloma viruses (10). Hands transiently contaminated with viruses can, however, act as vehicles for the spread of many types of viral infections. Hand hygiene is meant to reduce the load of viruses and other transient microorganisms on hands, thereby reducing the risk of disease transmission. Such reductions in the virus load may be due to a combination of virus inactivation and mechanical removal of infectious virus from the skin.1.2 This test method is designed to determine the comparative virus-eliminating effectiveness of microbicidal or non-microbicidal formulations. This test method is not meant for use with surgical hand scrubs or preoperative skin preps.NOTE 1: The test method should be performed by persons with training in virology in facilities designed and equipped for work with infectious agents at biosafety level 2 (11).1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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1.1 The guide provides information and clarity to support the health and maintenance of Marine Sanitation Devices (MSDs) on maritime vessels and platforms to promote effective operations and performance throughout the lifecycle. This includes identification of chemicals and their derivatives that can be detrimental to proper MSD operations. This guide will promote better understanding of the impacts of certain chemicals on the health and operations of MSD systems, plus provide guidance to inform operators of best practices and procedures for effective operations and maintenance. This guide is designed to assist both operators and MSD Original Equipment Manufacturers (OEMs) in collaboratively working to ensure effective operations and maintenance, and to reduce performance degradations that result from the introduction of harmful chemicals. The primary application of this guide is to Type II MSDs (described in Section 4), installed on larger ships and employing biological treatment of sewage and gray water.NOTE 1: This guide does not constitute regulations or ship classification society rules, which should be consulted where applicable.1.2 Manufacturers preparing new product specifications or revising existing ones should follow the practices and procedures outlined herein, and be guided by the latest specifications covering similar commodities. Similarly, vessel owner/operators should consult this guide regarding in-service operations and maintenance.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 The different procedures and methods are designed to be used to produce survival data after microorganisms are exposed to antimicrobial agents in order to calculate values that can be used to analyze and rationalize the effectiveness of antimicrobial agents when tested using other, often applied test methods.5.2 The data from these test procedures may be used in the selection and design of other tests of effectiveness of antimicrobial agents, some of which may be required by regulatory agencies to establish specific claims. Basic kinetic information about killing rate often serves as the initial information on which a testing program can be built.1.1 This guide covers the methods for determining the death rate kinetics expressed as D-values. These values can be derived from the construction of a kill curve (or survivor curve) or by using other procedures for determining the number of survivors after exposure to antimicrobial chemicals or formulations. Options for calculations will be presented as well as the method for calculation of a concentration coefficient.1.1.1 The test methods are designed to evaluate antimicrobial agents in formulations to define a survivor curve and to subsequently calculate a D-value. The tests are designed to produce data and calculate values that provide basic information of the rate-of-kill of antimicrobial formulations tested against single, selected microorganisms. In addition, calculated D-values from survivor curves from exposure at different dilutions of antimicrobial can be used to show the effect of dilution by calculation of the concentration exponent, η (2). D-value determination assumes the ideal of first-order killing reactions that are reflected in a straight-line reduction in count where a count-versus-time plot is done. The goal here is not to determine the time at which no survivors are found, but to determine a standard value that can be used in processing and exposure determinations or used to estimate dilutions.1.1.2 As an example of potential use of kill curve data, the published FDA, OTC Tentative Final Monograph for Health-Care Antiseptic Drug Products, Proposed Rule, June 17, 1994 has suggested the testing of topically applied antimicrobial products using survival curve (or kill curve) calculations. The methods described in this guide are applicable to these products, but adjustments such as the use of antifoaming agents when the reaction mixture is stirred may be necessary to counteract the presence of detergents in many formulations. Frequently the sampling for these tests is done after very short intervals of exposure to the formulation, such as 30 and 60 s. This methodology also has been applied to preservative testing of antimicrobial ingredients in more complex cosmetic formulations (5).1.2 The test methods discussed should be performed only by those trained in microbiological techniques.1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 These practices should be used only to collect visible samples that are suspected biological agents and toxins and have been field screened as defined by the FBI-DHS-HHS/CDC Coordinated Document for explosive hazard, radiological hazard, and other acute chemical hazards. 4.2 These practices provide standardized methods for collecting, packaging, and transporting suspicious visible powder samples that are suspected biological agents and toxins. Collection of a bulk powder material from a nonporous surface using a sterile swab and laminated card as the collection devices to move the material into a container will depend on several factors, including (but not limited to): (1) amount of visible powder present; (2) sample composition; (3) choice of collection device; (4) size and shape of the collection container; (5) ability of the powder to become aerosolized; (6) texture and porosity of the surface; (7) humidity; (8) air movement; and (9) electrostatic properties of powders and collection tools/containers. 4.3 Similarly, these practices standardize methods for sampling suspicious visible powders for on-site analysis, although wipe and swab sampling is often employed in the field for subsequent LRN reference laboratory analysis. The ability to collect suitable samples from nonporous surfaces using a sterile moistened swab will depend on the following factors: (1) swabbing procedure; (2) swab material; (3) sample composition; and (4) texture of the surface. 4.4 These practices standardize suspicious powder collection and packaging procedures and swab sampling procedures in order to reduce exposure risk, to reduce variability associated with sample handling and sample analysis, and to increase reliability of sampling visible powder samples from nonporous surfaces. 4.5 The bulk sample collection practice and the swab sampling practice are recommended for collecting amassed or dispersed powder samples from all nonporous surfaces on which the suspicious powder sample is clearly visible. 4.6 These practices are not recommended for samples on porous materials such as upholstery, carpeting, air filters, or ceiling tiles. 4.7 These practices are recommended for collecting visible powders where the bulk of the powder sample is amassed or dispersed over a limited area (optimally, area should be less than 20 by 20 cm (approximately 8 by 8 in.) or 400 cm2 (approximately 64 in.2). 4.8 These practices are to be performed by personnel who are adequately trained to work with hazardous materials in the hot zone (see NFPA 472, or OSHA - 29 CFR 1910.120). Personnel performing collection or screening under these practices shall be adequately trained in the use of sampling equipment, materials, and procedures. This includes personnel performing the prior initial chemical and radiological screening. Personnel should use the appropriate level of personal protective equipment (PPE) to mitigate hazards during collection and screening. Personnel performing collection or screening under these practices shall be aware of evidence preservation and sampling procedures (NFPA 472 section 6.5). 4.9 These standard practices should be used in accordance with Guide E2770 for best practices for planning, training and evaluation of competency. 1.1 These practices address collection of visible powders that are suspected biological agents and toxins from solid nonporous surfaces using a bulk collection method, using a dry swab and laminated card, followed by a swab sampling method using a sterile moistened swab. Bulk powder samples are collected and packaged in a manner that permits the maximum amount of the sample to be safely transported to a reference laboratory within the Centers for Disease Control and Prevention (CDC) national Laboratory Response Network (LRN)2 for confirmatory identification and safe storage. If the source of the powder is a letter or small package, that item is also packaged in a manner that permits it to be safely transported to an LRN reference laboratory. A sterile moistened swab may be used to collect residual powder from the nonporous surface and may be used to conduct on-site biological assessments for the purpose of testing for biological agents and toxins. 1.2 These practices are performed in coordination with the Federal Bureau of Investigation (FBI) as part of a risk assessment including hazard assessment and threat credibility evaluation as recommended and clarified in Guide E2770. The decision to implement these practices and collect a public safety sample will be made by members of the response community of the jurisdiction assuming responsibility through coordination with the FBI and the receiving LRN reference laboratory. 1.3 Sample Collection Method A covers the bulk collection and packaging of suspicious visible powders that are suspected biological agents and toxins from solid nonporous surfaces. All samples suspected to be biological agents and toxins on nonporous surfaces should be collected according to Sample Collection Method A and sent to an LRN reference laboratory for confirmatory testing. 1.4 Sample Collection Method B covers swab sampling of residual suspicious powders that are suspected biological agents and toxins from solid nonporous surfaces. Swab samples can be used for on-site biological assessment; however results from on-site biological assessments are not definitive; confirmatory testing by the LRN reference laboratory is necessary to make public health decisions. 1.5 These practices incorporate reference guidance for packaging and transport of suspicious visible powders to comply with all appropriate federal regulations regarding biosafety and biosecurity. 1.6 These practices should only be used to collect visible samples that are suspected biological agents and toxins and have been field screened according to reference guidance for explosive hazard, radiological hazard, and other acute chemical hazards. 1.7 The values stated in SI units are to be regarded as standard. The values given in parentheses are for information only. 1.8 This standard does not purport to address all of the safety concerns associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. 1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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1.1 The purpose of this terminology standard is to establish uniformity in terms used in the field of antimicrobial and antiviral agent testing. Terms are adapted from related fields such as regulatory terms defined by law and definitions as supported by test requirements.1.2 The terms are appropriate to the wide range of interest related to standards developed in the area of antimicrobial and antiviral testing.1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 This test method is intended for products sold as tank mix adjuvants for use with agricultural products.4.2 Part A provides a determination of whether the pH modification characteristics of the adjuvant tested meet the definition of an acidifier, alkalinity agent, or a basic blend in Terminology E1519. Part B provides a measure of the buffer capacity of the adjuvant. This can be used to estimate the amount of a buffer that will be required to keep a spray solution at the desired pH.4.3 Other pH standards can be used (see Test Method E70) as long as the pHs measured are not outside the range of the standards used.4.4 If samples of the material to be used in a particular application are available, the first method can be used to determine what adjuvant rate is required to obtain the desired pH. The ability of an adjuvant to adjust the pH of a particular system will depend on the initial pH before addition.4.5 The second method can be used to determine the amount of an adjuvant required to change the pH of a quantity of spray solution to the required value (see Note 1).NOTE 1: Many acidifiers and basic blends also are buffers, so the pH change does not provide a useful measure of buffer capacity.1.1 These test methods cover determining if an adjuvant meets the definitions of an acidifier, a basic blend, alkalinity agent, or buffer in Terminology E1519.1.2 The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system may not be exact equivalents; therefore, each system shall be used independently of the other. Combining values from the two systems may result in non-conformance with the standard.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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