5.1 The bioavailability of chemical elements is poorly related to the chemical composition of soils and plant growth media containing a mineral or any type of adsorbed phase. The chemical potential (pi for element, i,) is an intensity parameter (I), and the sorbed amount in equilibrium with the soil solution is a measure of the quantity (Q). These parameters for each element (essential or toxic) should be measured in the presence of other elements at or near the desired intensity. This test method is the only method that generates these results simultaneously for several elements. The computer software allows these values to be related to the total sorbed quantities of the different elements. For many substrates, it has been found that the theory for the method holds to the degree that vegetation has been established on many non-soil substrates and soil-water-food chain problems have been evaluated by this test method. This test method has been used on many sites in Pennsylvania and other locations to monitor the effect of sewage sludge applications on land as a source of essential elements for plants with no harmful effects on the food chain. It has also been used to evaluate synthetic soils produced from fly-ash alone or as a component of coal refuse for the establishment of vegetation on mine spoils, coal refuse piles, and abandoned mine lands.Note 1—The quality of the result produced by this standard is dependent on the competence of the personnel performing it, and the suitability of the equipment and facilities used. Agencies that meet the criteria of Practice D3740 are generally considered capable of competent and objective testing/sampling/inspection and the like. Users of this standard are cautioned that compliance with Practice D3740 does not in itself assure reliable results. Reliable results depend on many factors; Practice D3740 provides a means of evaluating some of those factors.1.1 This test method covers the determination of quantity (Q) and intensity (I) results for several elements in soils, spoils, fly-ash, and other soil substitutes to ascertain their suitability for the growth of vegetation and possible adverse effects of metals on the food chain.1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.3 All measured and calculated values shall conform to the guidelines for significant digits and rounding established in Practice D6026.1.4 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

定价： 0元 / 折扣价： 0 元

4.1 For many structural ceramic components in service, their use is often limited by lifetimes that are controlled by a process of SCG. This test method provides the empirical parameters for appraising the relative SCG susceptibility of ceramic materials under specified environments. Furthermore, this test method may establish the influences of processing variables and composition on SCG as well as on strength behavior of newly developed or existing materials, thus allowing tailoring and optimizing material processing for further modification. In summary, this test method may be used for material development, quality control, characterization, and limited design data generation purposes. The conventional analysis of constant stress rate testing is based on a number of critical assumptions, the most important of which are listed in the next paragraphs.4.2 The flexural stress computation for the rectangular beam test specimens or the equibiaxial disk flexure test specimens is based on simple beam theory, with the assumptions that the material is isotropic and homogeneous, the moduli of elasticity in tension and compression are identical, and the material is linearly elastic. The average grain size should be no greater than one-fiftieth of the beam thickness.4.3 The test specimen sizes and fixtures for rectangular beam test specimens should be in accordance with Test Method C1161, which provides a balance between practical configurations and resulting errors, as discussed in Refs (4, 5). Only four-point test configuration is allowed in this test method for rectangular beam specimens. Three-point test configurations are not permitted. The test specimen sizes and fixtures for disk test specimens tested in ring-on-ring flexure should be chosen in accordance with Test Method C1499. The test specimens for direct tension strength testing should be chosen in accordance with Test Method C1273.4.4 The SCG parameters (n and D) are determined by fitting the measured experimental data to a mathematical relationship between strength and applied stress rate, log σf = 1/(n+1) log σ˙ + log D. The basic underlying assumption on the derivation of this relationship is that SCG is governed by an empirical power-law crack velocity, v = A[KI/KIC]n (see Appendix X1).NOTE 3: There are various other forms of crack velocity laws which are usually more complex or less convenient mathematically, or both, but may be physically more realistic (6). It is generally accepted that actual data cannot reliably distinguish between the various formulations. Therefore, the mathematical analysis in this test method does not cover such alternative crack velocity formulations.4.5 The mathematical relationship between strength and stress rate was derived based on the assumption that the slow crack growth parameter is at least n ≥ 5 (1, 7, 8). Therefore, if a material exhibits a very high susceptibility to SCG, that is, n < 5, special care should be taken when interpreting the results.4.6 The mathematical analysis of test results in accordance with the method in 4.4 assumes that the material displays no rising R-curve behavior. It should be noted that the existence of such behavior cannot be determined from this test method.4.7 Slow crack growth behavior of ceramic materials exposed to stress-corrosive gases or liquid environments can vary as a function of mechanical, material, and electrochemical variables. Therefore, it is essential that test results accurately reflect the effects of specific variables under study. Only then can data be compared from one investigation to another on a valid basis or serve as a valid basis for characterizing materials and assessing structural behavior.4.8 The strength of advanced ceramics is probabilistic in nature. Therefore, SCG that is determined from the strengths of a ceramic material is also a probabilistic phenomenon. Hence, a proper range and number of applied stress rates in conjunction with an appropriate number of specimens at each applied stress rate are required for statistical reproducibility and design (2). Guidelines are provided in this test method.NOTE 4: For a given ceramic material/environment system, the SCG parameter n is constant regardless of specimen size although its reproducibility is dependent on the variables mentioned in 4.8. By contrast, the SCG parameter D depends significantly on strength and thus on specimen size (see Eq X1.6 in Appendix X1).4.9 The strength of a ceramic material for a given specimen and test fixture configuration is dependent on its inherent resistance to fracture, the presence of flaws, and environmental effects. Analysis of a fracture surface, fractography, though beyond the scope of this test method, is highly recommended for all purposes, especially to verify the mechanism(s) associated with failure (refer to Practice C1322).4.10 The conventional analysis of constant stress rate testing is based on a critical assumption that stress is uniform throughout the test piece. This is most easily achieved in direct tension test specimens. Only test specimens that fracture in the inner gauge section in four-point testing should be used. Three-point flexure shall not be used. Breakages between the outer and inner fixture contact points should be discounted. The same requirement applies to biaxial disk strength testing. Only fractures which occur in the inner loading circle should be used. Furthermore, it is assumed that the fracture origins are near to the tensile surface and do not grow very large relative to the thickness of rectangular beam flexure or disk strength test specimens.4.11 The conventional analysis of constant stress rate testing is also based on a critical assumption that the same type flaw controls strength in all specimens at all loading rates. If the flaw distribution is multimodal, then the conventional analysis in this standard may produce erroneous slow crack growth parameter estimates.1.1 This test method covers the determination of slow crack growth (SCG) parameters of advanced ceramics by using constant stress rate rectangular beam flexural testing, ring-on-ring biaxial disk flexural testing, or direct tensile strength, in which strength is determined as a function of applied stress rate in a given environment at ambient temperature. The strength degradation exhibited with decreasing applied stress rate in a specified environment is the basis of this test method which enables the evaluation of slow crack growth parameters of a material.NOTE 1: This test method is frequently referred to as “dynamic fatigue” testing (1-3)2 in which the term “fatigue” is used interchangeably with the term “slow crack growth.” To avoid possible confusion with the “fatigue” phenomenon of a material which occurs exclusively under cyclic loading, as defined in Terminology E1823, this test method uses the term “constant stress rate testing” rather than “dynamic fatigue” testing.NOTE 2: In glass and ceramics technology, static tests of considerable duration are called “static fatigue” tests, a type of test designated as stress rupture (See Terminology E1823).1.2 Values expressed in this test method are in accordance with the International System of Units (SI) and IEEE/ASTM SI 10.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 646元 / 折扣价： 550 元 加购物车

5.1 An accelerated test for determining the resistance of interior coated building products to mold growth is useful in estimating the relative performance for use in interior environments under conditions favorable to fungal growth.5.2 Static or environmental chambers provide controlled laboratory micro-environment conditions. These chambers are not intended to duplicate room conditions, and care must be taken when interpreting the results. Static chambers are not a substitute for dynamic chambers or field studies.1.1 This test method covers an environmental chamber and the conditions of operation to evaluate in a 4-week period the relative resistance to mold growth and microbial surface defacement on coated building products designed for interior application using an indirect inoculation method. The apparatus is designed so it can be easily built or obtained by any interested party.1.2 This test method can be used to evaluate the comparative resistance of coated building products to accelerated mold growth. Ratings do not imply a specific time period that the coated building product will be free of fungal growth during installation in an interior environment.1.3 This test method is not intended for use in the evaluation of public health claims.1.4 The test method is intended for the accelerated evaluation of mold growth on a coated building product designed for interior use. This method is not intended for evaluation of surfaces designed for exterior applications or uncoated surfaces. Use of this test method for evaluating exterior performance has not been validated, nor have the limitations for such use been determined.1.5 The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system may not be exact equivalents; therefore, each system shall be used independently of the other. Combining values from the two systems may result in non-conformance with the standard.1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

4.1 The test gives an estimate of the ability of a rubber vulcanizate to resist crack growth of a pierced specimen when subjected to bending or flexing.4.2 No exact correlation between these test results and service is implied due to the varied nature of service conditions.1.1 This test method covers the determination of crack growth of vulcanized rubber when subjected to repeated bending strain or flexing. It is particularly applicable to tests of synthetic rubber compounds which resist the initiation of cracking due to flexing when tested by Method B of Test Methods D430. Cracking initiated in these materials by small cuts or tears in service, may rapidly increase in size and progress to complete failure even though the material is extremely resistant to the original flexing-fatigue cracking. Because of this characteristic of synthetic compounds, particularly those of the SBR type, this test method in which the specimens are first artificially punctured in the flex area should be used in evaluating the fatigue-cracking properties of this class of material.1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

6.1 The environmental chamber method is an accelerated test for determining the resistance of Wet Blue and Wet White to the growth of fungi, the causal agent of mold. See Test Method D3273.3,46.2 The environmental chamber method is useful in estimating the performance of fungicides and should assist in the prediction of storage time before fungal growth begins.6.3 The environmental chamber method duplicates the natural environment in which Wet Blue or Wet White is inoculated with fungal spores and subsequently disfigured or discolored by fungi.6.4 The environmental chamber method measures the resistance of the treated Wet Blue or Wet White to the germination of spores and subsequent vegetative growth that spreads over the surface of a comparatively large Wet Blue or Wet White specimen over a period of four weeks.6.5 The environmental chamber can be kept inoculated with fungi representative of those found in tanneries by adding samples of Wet Blue and Wet White with fungal growth from currently operating tanneries.6.6 Control specimens of Wet Blue and Wet White without fungicide treatment can be added to the chamber periodically to increase levels of fungal growth in the chamber.6.7 Leaching of fungicide from the test specimen into the agar often causes a zone of inhibition of fungal growth in the Petri dish test, but in the environmental chamber any leaching of fungicide from the test specimen drips into the water contained in the chamber and thus does not cause the types of false readings observed in the Petri dish test.1.1 This environmental chamber method measures the resistance of the treated Wet Blue and Wet White to the germination of spores and subsequent vegetative growth over a period of four weeks. The test method is useful in estimating the performance of fungicides and should assist in the prediction of storage time of Wet Blue and Wet White before fungal growth begins. The apparatus is designed so it can be easily built or obtained by any interested party and duplicate the natural environment in which Wet Blue and Wet White is inoculated with fungal spores. Spores that germinate on untreated or treated Wet Blue and Wet White can produce fungal growth, resulting in disfigurement or discoloration, or both, of the Wet Blue and Wet White.1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

5.1 This test method evaluates the effect of an ECP on seed germination and initial plant growth in a controlled environment.5.2 The results of this test can be used to compare ECPs and other erosion control materials to determine which are the most effective at encouraging the growth of vegetation.1.1 This test method covers guidelines, requirements, and procedures for evaluating the effect of Erosion Control Products (ECPs) on seed germination and vegetation enhancement.1.2 This test method will evaluate the effects of both rolled erosion control products (RECPs) and hydraulically-applied erosion control products (HECPs) on seed germination in a controlled environment.1.3 This test method utilizes bench-scale testing procedures and shall not be interpreted as indicative of field performance.1.4 This test method is not intended to replace full-scale simulation or field testing in acquisition of performance values that are required in the design of erosion control measures utilizing RECPs and HECPs.1.5 The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system may not be exact equivalents; therefore, each system shall be used independently of the other. Combining values from the two systems may result in non-conformance with the standard.1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

定价： 590元 / 折扣价： 502 元 加购物车

6.1 Creep crack growth rate expressed as a function of the steady state C* or K characterizes the resistance of a material to crack growth under conditions of extensive creep deformation or under brittle creep conditions. Background information on the rationale for employing the fracture mechanics approach in the analyses of creep crack growth data is given in (11, 13, 30-35). 6.2 Aggressive environments at high temperatures can significantly affect the creep crack growth behavior. Attention must be given to the proper selection and control of temperature and environment in research studies and in generation of design data. 6.2.1 Expressing CCI time, t0.2 and CCG rate, da/dt as a function of an appropriate fracture mechanics related parameter generally provides results that are independent of specimen size and planar geometry for the same stress state at the crack tip for the range of geometries and sizes presented in this document (see Annex A1). Thus, the appropriate correlation will enable exchange and comparison of data obtained from a variety of specimen configurations and loading conditions. Moreover, this feature enables creep crack growth data to be utilized in the design and evaluation of engineering structures operated at elevated temperatures where creep deformation is a concern. The concept of similitude is assumed, implying that cracks of differing sizes subjected to the same nominal C*(t), Ct, or K will advance by equal increments of crack extension per unit time, provided the conditions for the validity for the specific crack growth rate relating parameter are met. See 11.7 for details. 6.2.2 The effects of crack tip constraint arising from variations in specimen size, geometry and material ductility can influence t0.2 and da/dt. For example, crack growth rates at the same value of C*(t), Ct in creep-ductile materials generally increases with increasing thickness. It is therefore necessary to keep the component dimensions in mind when selecting specimen thickness, geometry and size for laboratory testing. 6.2.3 Different geometries as mentioned in 1.1.6 may have different size requirements for obtaining geometry and size independent creep crack growth rate data. It is therefore necessary to account for these factors when comparing da/dt data for different geometries or when predicting component life using laboratory data. For these reasons, the scope of this standard is restricted to the use of specimens shown in Annex A1 and the validation criteria for these specimens are specified in 11.7. However if specimens other than the C(T) geometry are used for generating creep crack growth data, then the da/dt data obtained should, if possible, be compared against test data derived from the standard C(T) tests in order to validate the data. 6.2.4 Creep cracks have been observed to grow at different rates at the beginning of tests compared with the rates at equivalent C*(t), Ct or K values for cracks that have sustained previous creep crack extension (12, 13). This region is identified as ‘tail’. The duration of this transient condition, ‘tail’, varies with material and initially applied force level. These transients are due to rapid changes in the crack tip stress fields after initial elastic loading and/or due to an initial period during which a creep damage zone evolves at the crack tip and propagates in a self-similar fashion with further crack extension (12, 13). This region is separated from the steady-state crack extension which follows this period and is characterized by a unique da/dt versus C*(t), Ct or K relationship. This transient region, especially in creep-brittle materials, can be present for a substantial fraction of the overall life (35). Criteria are provided in this standard to quantify this region as an initial crack growth period (see 1.1.5) and to use it in parallel with the steady state crack growth rate data. See 11.8.8 for further details. 6.3 Results from this test method can be used as follows: 6.3.1 Establish predictive models for crack incubation periods and growth using analytical and numerical techniques (18-21). 6.3.2 Establish the influence of creep crack development and growth on remaining component life under conditions of sustained loading at elevated temperatures wherein creeps deformation might occur (23-28). Note 1: For such cases, the experimental data must be generated under representative loading and stress-state conditions and combined with appropriate fracture or plastic collapse criterion, defect characterization data, and stress analysis information. 6.3.3 Establish material selection criteria and inspection requirements for damage tolerant applications. 6.3.4 Establish, in quantitative terms, the individual and combined effects of metallurgical, fabrication, operating temperature, and loading variables on creep crack growth life. 6.4 The results obtained from this test method are designed for crack dominant regimes of creep failure and should not be applied to cracks in structures with wide-spread creep damage which effectively reduces the crack extension to a collective damage region. Localized damage in a small zone around the crack tip is permissible, but not in a zone that is comparable in size to the crack size or the remaining ligament size. Creep damage for the purposes here is defined by the presence of grain boundary cavitation. Creep crack growth is defined primarily by the growth of intergranular time-dependent cracks. Crack tip branching and deviation of the crack growth directions can occur if the wrong choice of specimen size, side-grooving and geometry is made (see 8.3). The criteria for geometry selection are discussed in 5.8. 1.1 This test method covers the determination of creep crack initiation (CCI) and creep crack growth (CCG) in metals at elevated temperatures using pre-cracked specimens subjected to static or quasi-static loading conditions. The solutions presented in this test method are validated for base material (that is, homogenous properties) and mixed base/weld material with inhomogeneous microstructures and creep properties. The CCI time, t0.2, which is the time required to reach an initial crack extension of δai = 0.2 mm to occur from the onset of first applied force, and CCG rate, a˙ or da/dt are expressed in terms of the magnitude of creep crack growth correlated by fracture mechanics parameters, C* or K, with C* defined as the steady state determination of the crack tip stresses derived in principal from C*(t) and Ct (1-17).2 The crack growth derived in this manner is identified as a material property which can be used in modeling and life assessment methods (17-28). 1.1.1 The choice of the crack growth correlating parameter C*, C*(t), Ct, or K depends on the material creep properties, geometry and size of the specimen. Two types of material behavior are generally observed during creep crack growth tests; creep-ductile (1-17) and creep-brittle (29-44). In creep ductile materials, where creep strains dominate and creep crack growth is accompanied by substantial time-dependent creep strains at the crack tip, the crack growth rate is correlated by the steady state definitions of Ct or C*(t) , defined as C* (see 1.1.4). In creep-brittle materials, creep crack growth occurs at low creep ductility. Consequently, the time-dependent creep strains are comparable to or dominated by accompanying elastic strains local to the crack tip. Under such steady state creep-brittle conditions, Ct or K could be chosen as the correlating parameter (8-14). 1.1.2 In any one test, two regions of crack growth behavior may be present (12, 13). The initial transient region where elastic strains dominate and creep damage develops and in the steady state region where crack grows proportionally to time. Steady-state creep crack growth rate behavior is covered by this standard. In addition, specific recommendations are made in 11.7 as to how the transient region should be treated in terms of an initial crack growth period. During steady state, a unique correlation exists between da/dt and the appropriate crack growth rate relating parameter. 1.1.3 In creep ductile materials, extensive creep occurs when the entire un-cracked ligament undergoes creep deformation. Such conditions are distinct from the conditions of small-scale creep and transition creep (1-10). In the case of extensive creep, the region dominated by creep deformation is significant in size in comparison to both the crack length and the uncracked ligament sizes. In small-scale-creep only a small region of the un-cracked ligament local to the crack tip experiences creep deformation. 1.1.4 The creep crack growth rate in the extensive creep region is correlated by the C*(t)-integral. The Ct parameter correlates the creep crack growth rate in the small-scale creep and the transition creep regions and reduces, by definition, to C*(t) in the extensive creep region (5). Hence in this document the definition C* is used as the relevant parameter in the steady state extensive creep regime whereas C*(t) and/or Ct are the parameters which describe the instantaneous stress state from the small scale creep, transient and the steady state regimes in creep. The recommended functions to derive C* for the different geometries shown in Annex A1 is described in Annex A2. 1.1.5 An engineering definition of an initial crack extension size δai is used in order to quantify the initial period of crack development. This distance is given as 0.2 mm. It has been shown (41-44) that this initial period which exists at the start of the test could be a substantial period of the test time. During this early period the crack tip undergoes damage development as well as redistribution of stresses prior reaching steady state. Recommendation is made to correlate this initial crack growth period defined as t0.2 at δai = 0.2 mm with the steady state C* when the crack tip is under extensive creep and with K for creep brittle conditions. The values for C* and K should be calculated at the final specified crack size defined as ao + δai where ao is initial size of the starter crack. 1.1.6 The recommended specimens for CCI and CCG testing is the standard compact tension specimen C(T) (see Fig. A1.1) which is pin-loaded in tension under constant loading conditions. The clevis setup is shown in Fig. A1.2 (see 7.2.1 for details). Additional geometries which are valid for testing in this procedure are shown in Fig. A1.3. These are the C-ring in tension CS(T), middle crack specimen in tension M(T), single edge notched tension SEN(T), single edge notched bend SEN(B), and double edge notched tension DEN(T). In Fig. A1.3, the specimens’ side-grooving-position for measuring displacement at the force-line displacement (FLD) and crack mouth opening displacement (CMOD) and positions for the electric potential drop (EPD) input and output leads are shown. Recommended loading for the tension specimens is pin-loading. The configurations, size range are given in Table A1.1 of Annex A1, (43-47). Specimen selection will be discussed in 5.9. 1.1.7 The state-of-stress at the crack tip may have an influence on the creep crack growth behavior and can cause crack-front tunneling in plane-sided specimens. Specimen size, geometry, crack length, test duration and creep properties will affect the state-of-stress at the crack tip and are important factors in determining crack growth rate. A recommended size range of test specimens and their side-grooving are given in Table A1.1 in Annex A1. It has been shown that for this range the cracking rates do not vary for a range of materials and loading conditions (43-47). Suggesting that the level of constraint, for the relatively short term test durations (less than one year), does not vary within the range of normal data scatter observed in tests of these geometries. However, it is recommended that, within the limitations imposed on the laboratory, that tests are performed on different geometries, specimen size, dimensions and crack size starters. In all cases a comparison of the data from the above should be made by testing the standard C(T) specimen where possible. It is clear that increased confidence in the materials crack growth data can be produced by testing a wider range of specimen types and conditions as described above. 1.1.8 Material inhomogeneity, residual stresses and material degradation at temperature, specimen geometry and low-force long duration tests (mainly greater that one year) can influence the rate of crack initiation and growth properties (42-50). In cases where residual stresses exist, the effect can be significant when test specimens are taken from material that characteristically embodies residual stress fields or the damaged material, or both. For example, weldments, or thick cast, forged, extruded, components, plastically bent components and complex component shapes, or a combination thereof, where full stress relief is impractical. Specimens taken from such component that contain residual stresses may likewise contain residual stresses which may have altered in their extent and distribution due to specimen fabrication. Extraction of specimens in itself partially relieves and redistributes the residual stress pattern; however, the remaining magnitude could still cause significant effects in the ensuing test unless post-weld heat treatment (PWHT) is performed. Otherwise residual stresses are superimposed on applied stress and results in crack-tip stress intensity that is different from that based solely on externally applied forces or displacements. Not taking the tensile residual stress effect into account will produce C* values lower than expected effectively producing a faster cracking rate with respect to a constant C*. This would produce conservative estimates for life assessment and non-conservative calculations for design purposes. It should also be noted that distortion during specimen machining can also indicate the presence of residual stresses. 1.1.9 Stress relaxation of the residual stresses due to creep and crack extension should also be taken into consideration. No specific allowance is included in this standard for dealing with these variations. However the method of calculating C* presented in this document which used the specimen’s creep displacement rate to estimate C* inherently takes into account the effects described above as reflected by the instantaneous creep strains that have been measured. However extra caution should still be observed with the analysis of these types of tests as the correlating parameters K and C* shown in Annex A2 even though it is expected that stress relaxation at high temperatures could in part negate the effects due to residual stresses. Annex A4 presents the correct calculations needed to derive J and C* for weldment tests where a mismatch factor needs to be taken into account. 1.1.10 Specimen configurations and sizes other than those listed in Table A1.1 which are tested under constant force will involve further validity requirements. This is done by comparing data from recommended test configurations. Nevertheless, use of other geometries are applicable by this method provided data are compared to data obtained from standard specimens (as identified in Table A1.1) and the appropriate correlating parameters have been validated. 1.2 The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are provided for information only and are not considered standard. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This guide presents options for a systematic assessment of fungal growth in buildings.5.2 This guide allows for site-specific flexibility and professional judgment in the choice of assessment procedures. It may not be necessary to perform in its entirety the basic assessment presented below to resolve a particular problem, for example, where fungal growth is localized and the source and extent of moisture is readily observable.5.3 Conversely, no matter how comprehensive the survey, all fungal growth may not be identified or located in a fungal assessment.5.4 Material removal or destructive investigation may be needed to access suspect surfaces.5.5 Using the procedures described in this guide, the investigator may have obtained the data necessary to suggest specific recommendations, for example, how to remediate the observed fungal growth, or how to prevent further fungal growth, but those recommendations are beyond the scope of this guide.5.6 Precautions may be needed to protect the assessor and building occupants where access may disturb fungal growth.5.7 It is the user’s responsibility to protect information that may be considered confidential, or private, or both, in accordance with project contract, corporate protocol, or local, state, and federal regulations, or a combination thereof.5.8 It may be necessary to enlist other disciplines or trade expertise to assist in some steps of the assessment, but recommendations of when to enlist and whom to enlist are beyond the scope of this guide.1.1 This guide provides a compendium of information and a menu of options for assessment of fungal growth in buildings, but does not recommend a specific course of action. Due to the wide variety of fungal problems affecting buildings and their occupants, and the wide variety of buildings, it is not possible to describe a set of uniform steps that will always be performed during an assessment (that is, a standard practice); therefore the user of this guide must decide which steps are appropriate for a given situation or building.1.2 This guide is specific to fungal growth, which is only one potential problem in a building environment. It may be part of, but is not intended to take the place of, a comprehensive indoor air quality investigation.1.3 This guide describes minimum steps and procedures for collecting background information on a building in question, procedures for evaluating the potential for moisture infiltration or collection, procedures for inspection for suspect fungal growth, and procedures beyond the scope of a basic survey that may be useful for specific problems.1.4 Assessments for fungal growth may be useful wherever fungal growth is suspected, excess moisture has been present or when there are concerns regarding potential fungal growth.1.5 Periodic fungal assessment in buildings may be a component of preventative maintenance programs.1.6 This guide is applicable to buildings including residential (for example, single or multi-family), institutional (for example, schools, hospitals), government, public assembly, commercial (for example, office, retail), and industrial facilities.1.7 Recommendations for developing a sampling strategy or methods for the collection and analysis of fungal samples are beyond the scope of this guide. For recommendations for developing a sampling strategy, see Ref (1)2, Chapter 10.1.8 Recommendations for remediation of fungal growth are beyond the scope of this guide.1.9 This guide is not intended to supersede any government regulations governing the assessment of fungal growth in buildings.1.10 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.11 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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3.1 An accelerated test for determining the resistance of interior coatings to mold growth is useful in estimating the performance of coatings designed for use in interior environments that promote mold growth and in evaluating compounds that may inhibit such growth and the aggregate levels for their use (see also Note 1).3.2 This test method should preferably be used by persons who have had basic microbiological training.1.1 This test method describes the use of an environmental chamber and operating conditions to evaluate the relative resistance of interior coatings to surface fungal growth in a severe interior environment during a 4-week period.1.2 This test method can be used to evaluate the comparative resistance of interior coatings to accelerated mold growth. Performance at a certain rating does not imply any specific period of time for a fungal free coating. However, a better rated coating nearly always performs better in actual end use.NOTE 1: This test method is intended for the accelerated evaluation of an interior coatings’ resistance to fungal defacement. Use of this test method for evaluating exterior coatings’ performance has not been validated, nor have the limitations for such use been determined. If this test method is to be used for the testing of an exterior coating system, a precautionary statement regarding interpretation of results as being outside of the scope of this test method must be included in the test report. Any accelerated weathering (leaching, weathering machine exposure, etc.) should be reported and should also bear reference to the fact that it is beyond the current scope of this test method.1.3 Temperature and humidity must be effectively controlled within the relatively narrow limits specified in order for the chamber to function reproducibly during the short test period. Severity and rate of mold growth on a film is a function of the moisture content of both the film and the substrate. A relative humidity of >93 % at a temperature of 32.5 ± 1 °C (90 ± 2 °F ) is necessary to initiate and maintain mold growth and for test panels to develop rapidly and maintain an adequate moisture level to support mold growth.1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 The growth of fungi in and on the surface of paint films represents a major cause of discoloration or disfigurement of painted surfaces. Because of their dark pigmentation, it is frequently difficult to distinguish fungi from dirt or soil particles.4.2 Use of Pictorial Standards:  4.2.1 The pictorial references that are part of this test method are for illustration purposes and may be used for visual comparisons.4.2.2 The diagrams represent an idealized schematic of various growth levels on paint films. They are intended as a representation only, but will serve as a useful guideline to establish amount and type of growth.4.2.3 The diagrams represented in Fig. 1 are not derived from a linear scale. The scale is intended to provide for more discrimination at the earlier stages of fungal or algal growth. It is at these levels that greater discernment is necessary.4.2.4 Comparisons made on dark colored substrates will be much more difficult, and will therefore require much more care and attention. It must be noted that because it is difficult to distinguish mild fungal or algal growth on the very dark substrates, there may be a tendency to under-rate those specimens.1.1 Fungal growth, frequently referred to as mildew in the paint industry, causes defacement of paint film exposed outdoors. The visual rating of paint surface disfigurement due to fungal or algal attack is required in order to compare the performance of different coatings.1.2 This method of rating mildew evaluation is intended to be used on exterior exposed paint films. This method may be used to rate interior fungal or algal growth, but it should be noted that the growth patterns on interior surfaces are different than exterior due to the lack of weathering influences. It is primarily intended for test specimens, but can also be used for rating mildew growth on larger structures such as entire houses. If this is used for large areas, the project should be broken down into smaller sections.1.3 This method is intended for field use for the macro rating of surface disfigurement only. The visual scales are meant to be used by the unaided eye to rate algal, fungal, or dirt disfigurement on larger surface areas such as test panels, siding boards, or entire buildings. Techniques are included for the differentiation of soil and dirt.1.4 Fungi will grow on most paint films exposed outdoors that are located in conditions favorable to growth. Test procedures such as Practices D1006, D3456, and G7 are available describing natural exposure tests that can be used to expose paint films, in order to create fungal or algal growth.1.5 The pictorial references available for use with this test method provide a numerical basis for rating the degree of fungal or algal growth on paint films.1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 These test methods are appropriate for qualifying absorbent pads used with membrane filters for bacteriological enumeration.5.1.1 The test methods described are applicable to quality control testing of absorbent pads by the suppliers and users of these pads and to specification testing of absorbent pads intended for use with membrane filters in bacteriological enumeration.5.2 Other pure culture organisms and their appropriate culture medium may be substituted for the E. coli and M-FC media for specification testing, as required.1.1 These test methods cover the determination of the nutrient-holding capacity and the toxic or nutritive effect on bacterial growth of organisms retained on a membrane filter, when the absorbent pad being tested is used as a nutrient reservoir and medium supply source for the retained bacteria.1.2 The tests described are conducted on 47 mm diameter disks, although other size disks may be employed for bacterial culture techniques.1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 Tissue-engineered cartilage is prepared by seeding stem cells or chondrocytes in a three-dimensional biodegradable scaffold under controlled growth conditions. It is expected that the cells will differentiate towards chondrogenic lineage and produce an ample amount of cartilage extracellular matrix proteins, proteoglycans, and collagen type-II. Longitudinal assessment is needed weekly for the first few weeks in vitro and monthly at a later stage in vivo to determine the growth rate of tissue-engineered cartilage. Traditional testing methods such as histological staining, mechanical testing, and qPCR are invasive, destructive, and cannot be performed in vivo after the transplantation of engineered tissue as a regenerative treatment. In the regenerative medicine of cartilage, it is important to evaluate whether the implanted tissue regenerates as an articular cartilage over time. MRI is the only available non-invasive imaging modality that is utilized for post-operative monitoring and assessment of cartilage regeneration in clinics. Therefore, it is important to evaluate tissue-engineered cartilage using MRI at the preclinical stage as well.4.7.1 The change in calculated relaxation rate, R2(ECM), using Eq 1 have been found to be positively correlated with tissue growth (3, 6).1.1 This standard is intended as a standard test method for engineered cartilage tissue growth evaluation using MRI.1.2 This standard is intended for use in the development of tissue engineering regenerative medical products for cartilage damages, such as in knee, hip, or shoulder joints.1.3 This standard has been prepared for evaluation of engineered cartilage tissue growth at the preclinical stage and summarizes results from tissue growth evaluation of tissue-engineered cartilage in a few notable cases using water spin-spin relaxation time, T2, in vitro and in vivo in small animal models.1.4 This standard uses the change in mean T2 values as a function of growth time to evaluate the tissue growth of engineered cartilage.1.5 This standard provides a method to remove the scaffold contribution to the tissue growth evaluation.1.6 Information in this standard is intended to be applicable to most porous natural and synthetic polymers used as a scaffold in engineered cartilage, such as alginate, agarose, collagen, chitosan, and poly-lactic-co-glycolic acid (PLGA). However, some materials (both synthetic and natural) may require unique or varied methods of MRI evaluation that are not covered in this test method.1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 The static chambers have several different applications:4.1.1 The static chambers can be used to compare the susceptibility of different materials to the colonization and amplification of various microorganisms under defined conditions.4.1.2 Chambers operated at high relative humidities may be used to perform worst case scenario screening tests on materials by providing an atmosphere where environmental conditions may be favorable for microbial growth.4.1.3 Use of multiple chambers with different environmental parameters, such as a range of relative humidities, permits the evaluation of multiple microenvironments and allows investigation of materials under differing environmental conditions.4.1.4 Drying requirements for wetted materials may also be investigated. This information may be relevant for determining material resistance to microbial growth after becoming wet. These conditions may simulate those where materials are subjected to water incursion through leaks as well as during remediation of a building after a fire.4.1.5 Growth rates of microorganisms on the material may also be investigated. Once it has been established that organisms are able to grow on a particular material under defined conditions, investigations into the rate of organism growth may be performed. These evaluations provide base line information and can be used to evaluate methods to limit or contain amplification of microorganisms.4.2 These techniques should be performed by personnel with training in microbiology. The individual must be competent in the use of sterile technique, which is critical to exclude external contamination of materials.1.1 Many different types of microorganisms (for example, bacteria, fungi, viruses, algae) can occupy indoor spaces. Materials that support microbial growth are potential indoor sources of biocontaminants (for example, spores and toxins) that can become airborne indoor biopollutants. This guide describes a simple, relatively cost effective approach to evaluating the ability of a variety of materials to support microbial growth using a small chamber method.1.2 This guide is intended to assist groups in the development of specific test methods for a definite material or groups of materials.1.3 Static chambers have certain limitations. Usually, only small samples of indoor materials can be evaluated. Care must be taken that these samples are representative of the materials being tested so that a true evaluation of the material is performed.1.4 Static chambers provide controlled laboratory microenvironment conditions. These chambers are not intended to duplicate room conditions, and care must be taken when interpreting the results. Static chambers are not a substitute for dynamic chambers or field studies.1.5 A variety of microorganisms, specifically bacteria and fungi, can be evaluated using these chambers. This guide is not intended to provide human health effect data. However, organisms of clinical interest, such as those described as potentially allergenic, may be studied using this approach.1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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1.1 This test method covers a procedure for measuring the cut growth resistance of precut vulcanizate specimens, under controlled conditions of environment, temperature, frequency, and severity. 1.2 The values stated in SI units are to be regarded as the standard. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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This practice covers the procedures used for detection of mycoplasma contamination of cell cultures by growth on agarose medium. This practice does not cover identification of mycoplasma and indirect methods for detection of mycoplasma. This practice will not detect cultivar strains of Mycoplasma hyorhinis nor intended for use in detection of mycoplasma contamination in sera, culture media, vaccines, or other systems. The practice involves DM-1 solid medium preparation, quality control, and mycoplasma isolation.1.1 This practice covers the procedures used for detection of mycoplasma contamination by direct microbiological culture.1.2 This practice does not cover indirect methods for detection of mycoplasma such as DNA staining, biochemical detection, or genetic probes.1.3 This practice does not cover methods for identification of mycoplasma organisms.1.4 This practice will not detect cultivar strains (1) of Mycoplasma hyorhinis.1.5 This practice is not intended for use in detection of mycoplasma contamination in sera, culture media, vaccines, or other systems.This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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