This guide details the standard methods suitable for testing latex vehicles. Included here are methods for latex sampling, nonvolatile content, minimum film formation temperature, qualitative polymeric analysis, density, viscosity, consistency, water content, pH, and surface tension.1.1 This guide covers methods suitable for testing latex vehicles. Certain of these methods were developed expressly for testing latex vehicles (Table 1). Others were developed for testing or analyzing formulated water- or solvent-based coatings but would be equally applicable for testing latices.1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 Latex paints are sometimes applied over substrates that contain a high level of water-soluble salts that results in efflorescence. This practice evaluates a coating’s vulnerability to efflorescence.1.1 This practice covers the evaluation of the degree to which a latex paint resists the formation of efflorescence and alkali burnout on the exposed paint surface.1.2 This practice is designed primarily to relate efflorescence originating in the substrate to the deposit appearing on the surface of latex paints. This practice relates chiefly to the painting of masonry-type substrates such as concrete block, brick, mortar, stucco, poured concrete, and similar materials.1.3 The values in SI units are to be regarded as the standard. The values in parentheses are for information only.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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1.1 This performance specification covers mixtures of water-based latex polymer, mineral aggregates, and optional other admixtures that, when combined, create a weather-protective and aliphatic hydrocarbon-resistant coating over asphalt and other pavements. There are two variations of the coating (Class 1 and Class 2): Class 1, a standard version—typically black but can be other colors, and Class 2, designed to be a solar reflective version.1.2 Units—The values stated in SI units are to be regarded as the standard. No other units of measurement are included in this standard.1.3 The text of this standard references notes and footnotes which provide explanatory material. These notes and footnotes (excluding those in tables and figures) shall not be considered as requirements for the standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 Weakening of natural rubber latex is known to occur after contact with certain lubricants, particularly petroleum-based products.3,4 This procedure was developed as a screening method for lubricant manufacturers to determine whether or not a particular personal lubricant has a significant effect on the tensile and airburst properties of an NRL condom.4.2 This test method is designed for use on NRL condoms that meet the criteria of Specification D3492 and can: (1) have a ring specimen cut in compliance with Appendix X1 of Specification D3492 and (2) be tested for burst properties in compliance with Annex A2 of Specification D3492.4.3 This test method is not to be used to determine the safety of either the test lubricant or NRL condom. This test method is to be used only to determine if the tensile or airburst properties of the NRL condom have been significantly affected by the test lubricant.1.1 This test method covers procedures used to detect a shift in physical properties of natural rubber latex (NRL) condoms after immersion in a personal lubricant. “Personal lubricants” are lubricants such as liquids or gels that are applied by the consumer at the time of condom use.1.2 This test method does not attempt to address compatibility of lubricants applied to a condom at the time of manufacture (“manufacturer lubricants”). It shall be the responsibility of the condom manufacturer to verify the long-term stability (shelf life) of any manufacturer lubricant that is packaged within the individual condom wrapper. Other regulatory requirements may apply.1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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1.1 This specification covers dipped goods and coatings made from compounded latex. Products manufactured from this material include boots, coated clips, coated sponge parts, and coated fabrics for automotive applications.1.2 The compounds listed in Tables 1 and 2 are grouped in classifications based primarily on physical properties, which are prescribed in the tables. These values, together with any additional requirements indicated by suffix letters in the grade designations as described in Section 2, define the properties of the compounds after vulcanization. These values apply to test specimens obtained from standard laboratory-dipped films prepared in accordance with procedures described in the applicable ASTM methods. Test results from finished products may not duplicate the values obtained from standard test films. When differences due to the difficulty in obtaining suitable test specimens from the finished part arise, the purchaser and the supplier may agree on acceptable deviations. This can be done by comparing results obtained on standard test films with those obtained on actual parts.1.3 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

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5.1 This test method measures the initial filtration efficiency of materials by sampling representative volumes of the upstream and downstream latex aerosol concentrations in a controlled airflow chamber.5.2 This test method provides specific test techniques for both manufacturers and users to evaluate materials when exposed to aerosol particle sizes between 0.1 and 5.0 μm.5.2.1 This test method establishes a basis of efficiency comparison between materials.1.1 This test method establishes procedures for measuring the initial particle filtration efficiency of materials using monodispersed aerosols.1.1.1 This test method utilizes light-scattering particle counting in the size range of 0.1 to 5.0 μm and airflow test velocities of 0.5 to 25 cm/s.1.2 The test procedure measures filtration efficiency by comparing the particle count in the feed stream (upstream) to that in the filtrate (downstream).1.3 The values stated in SI units or in other units shall be regarded separately as standard. The values stated in each system must be used independently of the other, without combining values in any way.1.4 The following precautionary caveat pertains only to the test methods portion, Section 10, of this specification. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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1.1 This specification covers the types, grades, and physical properties of aerosol polyurethane and aerosol latex foams extruded from pressurized containers and intended for building envelope air barrier sealant applications in building construction.1.2 For specific aerosol foam sealant applications, operational temperature limit criteria shall be as agreed upon between the aerosol sealant manufacturer and the purchaser.1.3 The values in SI units are to be regarded as standard. The values shown in parentheses are for information and approximation only.1.4 The committee with jurisdiction over this standard is not aware of any comparable standards published by other organizations.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 Interior flat paints may become burnished in areas where clothing or upholstered furniture rub against a wall. This rubbing may cause a smoother, glossier surface at the contacted area, depending on the level or type of pigments in the paint and binder hardness. This method permits a more quantitative estimate of burnish resistance than those using manual rubbing techniques.1.1 This test method covers a procedure for measuring the resistance of latex paints to burnishing under dry conditions.1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1  Dialkyldithiocarbamates (DTCs), benzothiazoles, and thiurams are often used as vulcanization accelerators in NRL products. Zinc DTC accelerators are added either directly or are formed in situ during the vulcanization process via reaction between a thiuram(s) and zinc oxide. DTCs, benzothiazoles, and thiurams have been detected in leachates from medical devices made of rubber such as gloves. Studies have shown these chemicals can cause allergic contact dermatitis. A simple selective method to monitor rubber accelerator levels in rubber extracts would be useful for quality control, product screening and research.5.2 This colorimetric assay measures dialkyldithiocarbamates, including zinc dialkyldithiocarbamates (ZDTC), mercaptobenzothiazole (MBT) and thiurams as a total thiol vulcanization accelerator level in rubber products. A UV spectrophotometer with detection at 320 nm is used to measure the ZDTC, mercaptobenzothiazole and thiurams. Sample extracts diluted at 1:20 prior to measurement on the spectrophotometer is usually sufficient to quantify the residual accelerator level from most commercially available rubber gloves; however, sample dilution can be adjusted (from neat extract to > 1:20 dilution) based on analytical needs. Thiurams and ZDTCs complex with cobalt turning the extract to a concentration-dependent shade of green. ZDTCs reacts quickly while thiurams react very slowly (requiring a heat catalyst). Mercaptobenzothiazole does not complex to Co(III), however, it absorbs strongly at 320 nm. It can be distinguished from both ZDTCs and thiurams by its strong absorbance at 320 nm without the cobalt dependent visible green color. Cobalt complexed thiurams and ZDTCs, but not MBT, also have and absorbance at 370 nm (2).1.1 This test method is designed to quantify the amount of total extractable accelerators in natural rubber latex (NRL) and nitrile gloves. Three common classes of rubber accelerators, the mercaptobenzothiazole (MBT), thiuram, and thiocarbamate type compounds can be detected and quantified by this method. If the specific rubber accelerator(s) present in the glove material is not known, quantification is based on zinc dibutyldithiocarbamate (ZDBC) equivalents. This method will not detect all potential rubber accelerators, including mercaptobenzothiazole disulfide, dimorpholine, thioureas and diphenyl diamine.1.2 For the purpose of this test method, the range of chemical accelerator measurement is based on the limit of detection (LOD) established in the performing laboratory.1.3 This test method should be performed by experienced analysts or under the supervision of those experienced in the use of spectroscopy and working with organic solvents.1.4 This test method has not been validated for measurement of long chain dithiocarbamates or accelerators from other rubber products, such as lubricated condoms (1).2 Although this assay has been reported in the literature for the evaluation of accelerator levels in condoms, further validation for accelerator measurement from other rubber products is required by the testing laboratory prior to use.1.5 This test method is not designed to evaluate the potential of rubber materials to induce or elicit Type IV skin sensitization reactions (for Type IV skin sensitization reactions see Test Method D6355). Total extractable accelerator content does not reflect bioavailablity of individual accelerators that are detected and measured by this method. This test method should be used to test and measure the total residual chemical accelerator level in NRL and nitrile gloves under controlled laboratory conditions, and should not be used to describe, appraise, or assess the hazard or risk of these materials or products under actual in-use conditions.1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This test method, for the determination of protein levels in latex, is primarily intended to test NR, latex, and elastomeric materials for residual protein content. It is assumed that all who use this test method will be trained analysts capable of performing common laboratory procedures skillfully and safely. It is expected that work will be performed in a properly equipped laboratory.1.1 This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with NR, latex, and elastomeric products. Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.1.2 For the purpose of this test method, the range of protein measurement will be based on the limit of detection and quantitation and recorded in micrograms per dm2 test specimen.1.3 The test method is designed to be accurate and compatible with the industrial environment.1.4 Steps are included in this test method to minimize the effects of interfering substances.1.5 It is recognized that other methods for the analysis of leachable proteins exist and these may be used for routine quality control purposes provided they have been validated and a correlation established against the reference method specified by this test method.1.6 This test method has not been validated for use with lubricated products such as condoms. Condoms with different lubricants as typically marketed, have not been tested in an ASTM ILS to determine if, and if so to what degree, the lubricant interferes with the assay.1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 As the brush drag of a paint increases, any natural tendency on the part of the painter to overspread the paint is reduced. When all other factors are held constant, increased brush drag will result in greater film thickness with consequent improvement in durability and hiding. Conversely, sometimes it might be preferred to have a lesser degree of brush drag for easier application (that is, the amount of time and effort in applying a paint to a specific area is reduced with a lesser degree of brush drag).5.2 This test method provides a standardized brushout procedure for the evaluation of brush drag as an alternative to customary informal ad hoc procedures. Its objective is to maximize the reliability and precision with which this characteristic may be determined.NOTE 1: The brush drag of paints is directly related to their high-shear viscosity. There is generally good rank order agreement between results obtained by this method and Test Method D4287. The sensitivity of this brushout method has been found sufficient to distinguish between brushability corresponding to high-shear viscosity differences not lower than 0.3 poise (0.03 Pa.s). Round robin data show that rank order agreement between the brushout and viscometric methods is poor when both latex and solvent-borne paints are part of the same comparison group. This is the result of these two paint types having markedly different rheological properties that affect the relative perception of brush drag.31.1 This test method is a standardized brushout procedure for comparing the brush drag of architectural type solvent-borne paints.1.2 With slight modifications this test method is also applicable to solvent-borne paints.1.3 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This practice can be used by paint formulators to determine if a given paint has the ability to form a film at low temperatures. This practice can be used as an aid for determining coalescing solvent level required to form a good film. Test Method D3793 compares the porosity of a film drawn down at room temperature to another film drawn down and cured at low temperature.5.2 This practice provides no absolute information, rather ranking only of the selected series of paints.5.3 This practice can provide a pass-fail situation if known acceptable and unacceptable paints are included in the test.1.1 This practice specifies a laboratory procedure for evaluating the ability of a latex paint to form a uniform film free of visible defects at low temperatures.1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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1.1 This classification covers descriptions of properties that may be observed in weathered latex paint films. These descriptions should be used in reporting the condition of such paint films. Note 1-In inspecting weathered exterior latex paints, defects or failures in the films may be observed that are not described in ASTM methods for evaluating the deterioration of exterior paints of the linseed-oil type.

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5.1 IgE-mediated allergic reactions to protein allergens in Hevea natural rubber latex derived from the Hevea brasiliensis tree emerged in the 1990s as a concern with occasional allergic manifestations. Symptoms encompassing hives, uriticaria, rhinitis, asthma and anaphylaxis have all been reported in latex allergic individuals exposed to products derived from Hevea natural rubber latex.5.2 Since no safe level of Hevea latex allergen exposure is known, avoidance is the primary mode of treating latex allergy.5.3 As a result of investigations conducted by many scientists across the world, fourteen latex allergens have so far been identified and categorized by the Allergen Nomenclature Sub-Committee of the International Union of Immunological Societies (IUIS) as Hev b 1 to Hev b 13 (Table 1) (see Specification D1193). Reported sensitization rates for these allergenic Hev b proteins vary among the many reports as a result of differences in the study populations, IgE antibody assay methods and the quality of the Hev b allergens used as calibrators and quality control reagents in the analysis. Most studies, however, agree that Hev b 1 and Hev b 3 are important allergens for individuals (for example, children with spina bifida) who are exposed through mucosal contact as a result of multiple surgeries or latex catheter use for an extended period of time. Additionally, investigators performing sensitization studies also agree that Hev b 5 and Hev b 6.02 are important allergens that may elicit sensitization in genetically-predisposed individuals who are exposed to Hevea natural rubber latex (2-4). On the basis of these clinical studies, assays for these four allergenic proteins (that is, Hev b 1, Hev b 3, Hev b 5 and Hev b 6.02) have been developed and they are thus the subject of this standard. Adoption of immunoenzymetric assay reagents and standard proteins needed to quantify other latex allergens (other than Hev b 1, 3, 5, and 6.02) in extracts of Hevea natural rubber latex products will require separate documentation and validation.(A) PR = pathogenesis-related.5.3.1 From the historical context, a number of assays have been developed to quantify the level of protein, antigen and allergen in Hevea natural rubber latex containing products (see Practices D4483 and D4678).5.3.2 The modified Lowry assay for total protein, Test Method D5712, was the first assay of this type. It assesses the level of total protein as an indirect indicator of allergenicity of latex-containing products. This assay does not discriminate between the allergenic and non-allergenic proteins.5.3.3 The second assay to be developed involved the use of human latex-specific IgE antibody in a competitive inhibition immunoassay format to estimate the overall allergenic potency of a Hevea natural rubber product extract (5, 6). The extract is incubated with human serum containing latex-specific IgE antibody and then this mixture is incubated with a solid phase latex allergosorbent. Latex allergenic proteins, if they are present, bind to the latex-specific IgE antibody in solution and they thus inhibit IgE antibody binding onto the latex allergosorbent. Allergosorbent bound IgE is then quantified and the extent of competitive inhibition of IgE binding is a measure of latex allergens. While this assay provides an estimate of the allergenicity or level of Hevea natural rubber allergens extractable from a product, difficulty in procuring reproducible lots of latex specific IgE containing human serum has precluded widespread use of this assay. For this reason, this assay has not been put forth as an ASTM standard.5.3.4 A third assay design is similar to the human IgE based competitive inhibition immunoassay, but it employs rabbit antiserum instead of human serum containing IgE anti-latex. The competitive inhibition enzyme linked immunosorbent assay (ELISA) has been adopted as Test Method D6499. It measures latex proteins that elicit immune responses, but it cannot distinguish between latex allergens (IgE inducing) from non-allergenic antigens (non-IgE inducing).5.3.5 The most recent assay, which is the subject of this standard, is the two-site immunoenzymetric assay (IEMA) which uses an insolubilized capture antibody to bind one of Hev b allergenic proteins from a latex product extract, and a second enzyme labeled detection antibody to detect bound allergens. Optical density responses are interpolated from reference curves constructed with known allergens. The performance characteristics of the reagents used in immunoenzymetric assays for Hev b 1, 3, 5 and 6.02 were investigated in the international collaborative study associated with the development of this standard and results are provided in Sections 15 through 17.1.1 This test method covers an immunological method known as an immunoenzymetric assay to quantify the amount of 4 principal Hevea brasiliensis [Hev b] allergenic proteins [Hev b 1, Hev b 3, Hev b 5 and Hev b 6.02] in Hevea natural rubber and its products2 derived from latex using monoclonal antibodies specific for epitopes on these proteins. Since these assays quantify the levels of only 4 of the known 14 officially acknowledged allergens potentially present in Hevea natural rubber latex containing products, the sum of the four allergen levels shall be viewed as an indicator of the allergen burden and not as a measure of the total allergen content that can be released from the product.1.2 For the purpose of this test method, the range of allergenic protein will be measured in terms of nanogram to microgram quantities per gram or unit surface area of a Hevea natural rubber containing product.1.3 The test method is not designed to evaluate the potential of Hevea natural rubber containing materials to induce or elicit Type I (IgE-mediated) hypersensitivity reactions.1.4 This test method should be used under controlled laboratory conditions to detect and quantify the level of 4 allergenic proteins found in Hevea natural rubber containing products. It should not be used to describe, appraise or assess the hazard or risk of these Hevea natural rubber containing materials or products under actual in use conditions.1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 Shrinkage of a sealant, after application in a building joint, is caused by loss of volatile components from the sealant. This loss results in a decrease in volume and, hence, a change in the sealant's shape. This change in shape, in some applications, should be taken into consideration for acceptable joint appearance and geometry.5.2 The shrinkage value obtained by this test method helps predict the appearance and geometry of the cured sealant in a building joint and is helpful in determining the amount and type of tooling to be done during installation of the sealant.5.3 Latex sealants cure primarily through water evaporation. They may also contain small amounts of other volatile components. However, in this test method all volatiles are treated as water. This assumption still provides a meaningful shrinkage value since the small quantities of other volatiles and their differences in density from that of water do not significantly affect the usefulness of the result obtained.1.1 This test method covers a laboratory procedure for determining volume shrinkage, which occurs during cure, of a latex sealant.1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are provided for information only.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.NOTE 1: A related ISO standard is ISO 10563. The user should compare to determine how it differs from this test method.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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