5.1 The practical life of an internal combustion engine is most often determined by monitoring its oil consumption. Excessive oil consumption is cause for engine repair or replacement and can be symptomatic of excessive wear of the piston ring or the cylinder bore or both. More wear-resistant materials of construction can extend engine life and reduce cost of operation. Although components made from more wear-resistant materials can be tested in actual operating engines, such tests tend to be expensive and time consuming, and they often lead to variable results because of the difficulty in controlling the operating environment. Although bench-scale tests do not simulate every aspect of a fired engine, they are used for cost-effective initial screening of candidate materials and lubricants. The test parameters for those tests are selected by the investigator, but the end result is a pair of worn specimens whose degree of wear needs to be accurately measured. The use of curved specimens, like segments of crowned piston rings, presents challenges for precise wear measurement. Weight loss or linear measurements of lengths and widths of wear scars may not provide sufficient accuracy to discriminate between small differences in wear. This guide is intended to address that problem.1.1 This guide describes a profiling method for use accurately measuring the wear loss of compound-curved (crowned) piston ring specimens that run against flat counterfaces. It does not assume that the wear scars are ideally flat, as do some alternative measurement methods. Laboratory-scale wear tests have been used to evaluate the wear of materials, coatings, and surface treatments that are candidates for piston rings and cylinder liners in diesel engines or spark ignition engines. Various loads, temperatures, speeds, lubricants, and durations are used for such tests, but some of them use a curved piston ring segment as one sliding partner and a flat or curved specimen (simulating the cylinder liner) as its counterface. The goal of this guide is to provide more accurate wear measurements than alternative approaches involving weight loss or simply measuring the length and width of the wear marks.1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 Use this test method to measure the thermal protection provided by different materials, garments, clothing ensembles, and systems when exposed to a specified fire (see 3.2.2, 3.2.3, 4.1, and 10.4).5.1.1 This test method does not simulate high radiant exposures, for example, those found in electric arc flash exposures, some types of fire exposures where liquid or solid fuels are involved, nor exposure to nuclear explosions.5.2 This test method provides a measurement of garment and clothing ensemble performance on a stationary upright manikin of specified dimensions. This test method is used to provide predicted skin burn injury for a specific garment or protective clothing ensemble when exposed to a laboratory simulation of a fire. It does not establish a pass/fail for material performance.5.2.1 This test method is not intended to be a quality assurance test. The results do not constitute a material’s performance specification.5.2.2 The effects of body position and movement are not addressed in this test method.5.3 The measurement of the thermal protection provided by clothing is complex and dependent on the apparatus and techniques used. It is not practical in a test method of this scope to establish details sufficient to cover all contingencies. Departures from the instructions in this test method have the potential to lead to significantly different test results. Technical knowledge concerning the theory of heat transfer and testing practices is needed to evaluate if, and which departures from the instructions given in this test method are significant. Standardization of the test method reduces, but does not eliminate, the need for such technical knowledge. Report any departures along with the results.1.1 This test method is used to provide predicted human skin burn injury for single-layer garments or protective clothing ensembles mounted on a stationary upright instrumented manikin which are then exposed in a laboratory to a simulated fire environment having controlled heat flux, flame distribution, and duration. The average exposure heat flux is 84 kW/m2 (2 cal/s·cm2), with durations up to 20 s.1.2 The visual and physical changes to the single-layer garment or protective clothing ensemble are recorded to aid in understanding the overall performance of the garment or protective clothing ensemble and how the predicted human skin burn injury results can be interpreted.1.3 The skin burn injury prediction is based on a limited number of experiments where the forearms of human subjects were exposed to elevated thermal conditions. This forearm information for skin burn injury is applied uniformly to the entire body of the manikin, except the hands and feet. The hands and feet are not included in the skin burn injury prediction.1.4 The measurements obtained and observations noted can only apply to the particular garment(s) or ensemble(s) tested using the specified heat flux, flame distribution, and duration.1.5 This standard is used to measure and describe the response of materials, products, or assemblies to heat and flame under controlled conditions, but does not by itself incorporate all factors required for fire hazard or fire risk assessment of the materials, products, or assemblies under actual fire conditions.1.6 This method is not a fire test response test method.1.7 The values stated in SI units are to be regarded as standard. The values given in parentheses are mathematical conversions to inch-pound units or other units commonly used for thermal testing. If appropriate, round the non-SI units for convenience.1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.9 Fire testing is inherently hazardous. Adequate safeguards for personnel and property shall be employed in conducting these tests.1.10 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This test method determines the effectiveness of UVGI devices for reducing viable microorganisms deposited on carriers.5.2 This test method evaluates the effect soiling agents have on UVGI antimicrobial effectiveness.5.3 This test method determines the delivered UVGI dose.1.1 This test method defines test conditions to evaluate ultraviolet germicidal irradiation (UVGI) light devices (mercury vapor bulbs, light-emitting diodes, or xenon arc lamps) that are designed to kill/inactivate influenza virus deposited on inanimate carriers.1.2 This test method defines the terminology and methodology associated with the ultraviolet (UV) spectrum and evaluating UVGI dose.1.3 This test method defines the testing considerations that can reduce UVGI surface kill effectiveness (that is, soiling).1.4 Protocols for adjusting the UVGI dose to impact the reductions in levels of viable influenza virus are provided (Annex A1).1.5 This test method does not address shadowing.1.6 The test method should only be used by those trained in microbiology and in accordance with the guidance provided by Biosafety in Microbiological and Biomedical Laboratories.21.7 This test method is specific to influenza viruses1.8 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.9 Warning—Mercury has been designated by many regulatory agencies as a hazardous substance that can cause serious medical issues. Mercury, or its vapor, has been demonstrated to be hazardous to health and corrosive to materials. Use caution when handling mercury and mercury-containing products. See the applicable product Safety Data Sheet (SDS) for additional information. The potential exists that selling mercury or mercury-containing products, or both, is prohibited by local or national law. Users must determine legality of sales in their location.1.10 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.11 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 The test method was designed to determine the LR in spores on a hard, non-porous surface after exposure to a test chemical in a closed system.5.2 Each test includes three control carriers (exposed to phosphate buffered saline with Tween-80), three test system control carriers (exposed to 1500 ppm  ± 150 ppm sodium hypochlorite), and ten treated carriers (per test chemical/concentration/contact time combination).1.1 This test method covers a standardized approach to quantitatively determine the effectiveness of antimicrobial chemicals in treating hard, non-porous surfaces contaminated with spores of C. difficile (ATCC 43598) grown in accordance with Practice E2839.1.2 This test method is based on principles established for Test Method E2197 and an Organisation for Economic Co-operation and Development Guidance Document.21.3 Training in basic microbiology and aseptic technique are required to perform this assay.1.4 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 The different procedures and methods are designed to be used to produce survival data after microorganisms are exposed to antimicrobial agents in order to calculate values that can be used to analyze and rationalize the effectiveness of antimicrobial agents when tested using other, often applied test methods.5.2 The data from these test procedures may be used in the selection and design of other tests of effectiveness of antimicrobial agents, some of which may be required by regulatory agencies to establish specific claims. Basic kinetic information about killing rate often serves as the initial information on which a testing program can be built.1.1 This guide covers the methods for determining the death rate kinetics expressed as D-values. These values can be derived from the construction of a kill curve (or survivor curve) or by using other procedures for determining the number of survivors after exposure to antimicrobial chemicals or formulations. Options for calculations will be presented as well as the method for calculation of a concentration coefficient.1.1.1 The test methods are designed to evaluate antimicrobial agents in formulations to define a survivor curve and to subsequently calculate a D-value. The tests are designed to produce data and calculate values that provide basic information of the rate-of-kill of antimicrobial formulations tested against single, selected microorganisms. In addition, calculated D-values from survivor curves from exposure at different dilutions of antimicrobial can be used to show the effect of dilution by calculation of the concentration exponent, η (2). D-value determination assumes the ideal of first-order killing reactions that are reflected in a straight-line reduction in count where a count-versus-time plot is done. The goal here is not to determine the time at which no survivors are found, but to determine a standard value that can be used in processing and exposure determinations or used to estimate dilutions.1.1.2 As an example of potential use of kill curve data, the published FDA, OTC Tentative Final Monograph for Health-Care Antiseptic Drug Products, Proposed Rule, June 17, 1994 has suggested the testing of topically applied antimicrobial products using survival curve (or kill curve) calculations. The methods described in this guide are applicable to these products, but adjustments such as the use of antifoaming agents when the reaction mixture is stirred may be necessary to counteract the presence of detergents in many formulations. Frequently the sampling for these tests is done after very short intervals of exposure to the formulation, such as 30 and 60 s. This methodology also has been applied to preservative testing of antimicrobial ingredients in more complex cosmetic formulations (5).1.2 The test methods discussed should be performed only by those trained in microbiological techniques.1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 The Single Tube Method is designed to evaluate the efficacy of disinfectants against biofilm grown in the CDC biofilm reactor following the procedures outlined in Practice E3161. Biofilm grown in the CDC reactor is representative of biofilm that forms under high fluid shear on surfaces conducive to biofilm formation.5.1.1 Vegetative biofilm bacteria are phenotypically different from suspended planktonic cells of the same genotype. Biofilm growth reactors are engineered to produce biofilm with specific characteristics (2). Altering either the engineered system or operating conditions will modify those characteristics as well as the physicochemical environment. The goal in biofilm research and testing is to choose the growth reactor and operating conditions that generate the most relevant biofilm for the particular study.5.2 The test method was designed to determine the log10 reduction in bacteria after exposure to a disinfectant in a closed system.5.3 The test method uses 50 mL conical tubes. The conical geometry allows for disinfectant exposure to biofilm on all surfaces of the coupon. For foaming disinfectants or for disinfectants requiring a larger volume of neutralizer, 250 mL conical tubes are used which preserve the required geometry and allow for greater neutralization capacity.5.4 Each test includes three untreated control coupons (exposed to buffered dilution water) and five treated coupons (per disinfectant/concentration/contact time combination).1.1 This test method specifies the operational parameters required to perform a quantitative liquid disinfectant efficacy test against bacterial biofilm.1.2 The test method was optimized and validated for a Pseudomonas aeruginosa or Staphylococcus aureus biofilm grown in the CDC Biofilm Reactor (E3161). The method is suitable for evaluating additional bacteria grown using the procedures outlined in methods with comparable coupon dimensions such as Practice E3161, Test Method E2562, or Test Method E2196.1.3 Disinfectant preparation and contact time are used in the assessment according to the manufacturer’s instructions for use.1.4 The test method uses a closed system to treat biofilm. A coupon is placed in a single tube for the treatment, neutralization, and harvesting steps to prevent the loss of cells.1.5 This test method describes a harvesting and analysis procedure which includes vortexing and sonicating treated and untreated control biofilm, and recovery of culturable cells using filtration to lower the limit of detection. Biofilm population density is recorded as log10 colony-forming units per coupon. Efficacy is reported as a log10 reduction of culturable cells.1.6 Basic microbiology training is required to perform this assay.1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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3.1 This guide is the first known attempt to focus on security requirements and compare them to available and known technologies capable of meeting these requirements. This guide describes several steps to select the appropriate anti-CADDSS technology. These steps are described in Section 4.1.1 This general guide is intended to assist the user of the guide in selecting anti-CADDSS technologies to protect their product from CADDSS.1.2 This guide does not address or evaluate specific anti-CADDSS technologies, but rather suggests a path that assists in the objective evaluation of features of anti-CADDSS technologies available protection of their product from CADDSS.1.3 This guide provides a procedure to accomplish the proper selection of a security system. Specific technologies are not addressed, nor are any technologies recommended. There are many security systems available in the public marketplace today. Each has limitations and must be carefully measured against the parameters presented in this guide. Once this careful analysis is done, the user will be in a knowledgeable position to select a security system to meet his needs.

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5.1 Methods such as D3273 Standard Test Method for Resistance to Growth of Mold on the Surface of Interior Coatings in an Environmental Chamber and D3274 Standard Test Method for Evaluating the Degree of Surface Disfigurement of Paint Films by Fungal or Algal Growth or Soil or Dirt Accumulation provide means for assessing mold and algal staining on paints. The Test Method E1428 Evaluating the Performance of Antimicrobials in or on Polymeric Solids Against Staining by Streptomyces species (A Pink Stain Organism) is used for solid polymeric materials, but is not appropriate for all antimicrobial technologies.5.2 This test method provides a technique for evaluating antimicrobials in or on polymeric materials against staining by Streptomyces species and should assist in the prediction of performance of treated articles under actual field conditions.1.1 This test method is intended to assess susceptibility of polymer materials, as well as products that may directly contact the treated polymer, to staining by the Actinomycete Streptomyces species.1.2 This test method is also suitable for evaluating dark-pigmented test samples since the bacterial growth inhibition can be assessed.1.3 Familiarity with microbiological techniques is required. This test method should not be used by persons without at least basic microbiological training.1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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