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5.1 This test method was developed to evaluate automotive engine oils for protection against oil thickening and piston deposits during moderately high-speed, hightemperature service.5.1.1 The increase in kinematic viscosity of the oil indicates the tendency of an oil to thicken because of oxidation. In automotive service, such thickening can cause oil pump starvation and resultant catastrophic engine failures.5.1.2 The deposit ratings for an oil indicate the tendency for the formation of deposits throughout the engine, including those that can cause sticking of the piston rings in their grooves. In automotive service, such ring sticking can cause a loss of compression pressures in the engine.5.2 The test method was developed to correlate with oils of known good and poor protection against oil thickening and piston deposits. Specially formulated oils that produce less than desirable results with unleaded fuels were also used during the development of this test.5.3 The Sequence IIIH engine oil test has been recommended as a replacement for the Sequence IIIG test and is expected to be used in specifications and classifications of engine lubricating oils, such as the following:5.3.1 Specification D4485.5.3.2 Military Specification MIL-PRF-2104.5.3.3 SAE Classification J183.1.1 This test method covers an engine test procedure for evaluating automotive engine oils for certain high-temperature performance characteristics, including oil thickening (as measured by kinematic viscosity increase), piston deposits, ring sticking, oil consumption, and phosphorus retention. Such oils include both single-viscosity and multiviscosity grade oils that are used in both spark-ignition, gasoline-fueled engines, as well as in diesel engines.1.1.1 Additionally, with nonmandatory supplemental requirements, a Sequence IIIHA Test (Mini Rotary Viscometer and Cold Cranking Simulator measurements), or a Sequence IIIHB Test (phosphorus retention measurement) can be conducted. These supplemental test procedures are contained in Appendix X1 and Appendix X2, respectively.NOTE 1: Companion test methods used to evaluate engine oil performance for specification requirements are discussed in SAE J304.1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.2.1 Exceptions: 1.2.1.1 Where there is no direct SI equivalent such as screw threads, national pipe threads/diameters, tubing sizes, and valve sizes and springs.1.2.1.2 The ring end gaps in Table A8.7, the dimensions for the blowby ventilation support bracket in Fig. A3.2, and the torque wrenches in Table A8.1 are in inch-pound units.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific warning statements are provided in 6.11.6, 7.1, 7.2.1, and 7.3.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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1.1 This practice covers the measurement of energy used for space conditioning and domestic water heating in both single and multifamily buildings. It is intended to assist those who design test methods for field monitoring projects. It establishes a uniform set of guidelines for specifying the data needed to evaluate energy used for space conditioning and domestic water heating in residential buildings, and is intended to promote the exchange of data and to improve measurement practices.1.2 This practice neither specifies the complete experimental design nor defines the data analysis technique to be used.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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3.1 These test methods are intended mainly for referee purposes but may be used for quality control of rubber production. They may also be used in research and development work and for comparison of different rubber samples in a standard formula.3.2 These test methods may be used to obtain values for customer control acceptance of rubber.1.1 These test methods cover the standard materials, test formulas, mixing procedures and test methods for the evaluation of various types of acrylonitrile-butadiene rubber (NBR).1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 The procedure in this test method should be used to evaluate the activity of the test formulation in reducing the bacterial population of the hands immediately after a single use and to determine persistent activity (inhibition of growth) after 6 h. Optionally, measurements of persistent activity after a 3 h period and measurements of cumulative activity may be made after repetitive uses over a five day period.1.1 This test method is designed to measure the reduction of microbial flora on the skin. It is intended for determining both immediate and persistent (continuing antimicrobial effect) microbial reductions, after single or repetitive treatments, or both. It may also be used to measure cumulative antimicrobial activity after repetitive treatments.1.2 A knowledge of microbiological techniques is required for these procedures.1.3 Performance of this procedure requires the knowledge of regulations pertaining to the protection of human subjects (21 CFR, Parts 50 and 56)1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.4.1 In this test method, SI units are used for all applications, except for distance, in which case inches are used and SI units follow in parentheses.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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3.1 These test methods are mainly intended for referee purpose but may be used for quality control of rubber production. They may also be used in research and development work and for comparison of different rubber samples in a standard formula.3.2 These test methods may be used to obtain values for acceptance of rubber.1.1 These test methods cover the standard materials, test formula, mixing procedures, and test methods for the evaluation and production control of non-halogenated isobutene-isoprene rubbers (IIR), commonly known as butyl rubber.1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This practice describes a procedure for preparing and storing a suspension of C. difficile spores that meets the following acceptance criteria: (1) spore titer of approximately 5.0×108 spores/mL, (2) spore purity of ≥95 %, and (3) a mean log10 reduction (LR) value >5.0 for 3 carriers exposed to 5000 ppm and a mean LR of <3.0 for 3 carriers exposed to 1500 ppm sodium hypochlorite. These acceptance criteria are necessary in order to use the spore suspension to evaluate the performance of antimicrobial formulations using Test Method E3218.1.1 This practice specifies the procedures for producing and storing standardized suspensions of Clostridioides difficile spores for the evaluation of the sporicidal activity of antimicrobial formulations using the Quantitative Method for Testing Antimicrobial Agents against Spores of C. difficile on Hard, Non-porous Surfaces or other procedures.1.2 This practice may involve hazardous materials, chemicals, and microorganisms and should be performed only by persons with formal training in microbiology.1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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3.1 These test methods are intended mainly for referee purposes but may be used for quality control of rubber production. They may also be used in research and development work and for comparison of different rubber samples in a standard formula.3.2 These tests may be used to obtain values for customer acceptance of rubber.1.1 These test methods cover the standard materials, test formula, mixing procedures, and test methods for evaluation of butadiene rubber (BR) made by polymerization in solution.1.2 The values stated in SI units are to be regarded as the standard. The values in parentheses are for information only.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 This test method is intended for referee purposes, but may be used for quality control, research and development testing and comparison of different rubber samples in a standard formula.4.2 This test method may also be used to obtain values for customer acceptance of rubber.4.3 This test method is a revision of Test Methods D3190 (prior to 1991), with 3-methyl thiazolidine thione-2 being substituted for ethylene thiourea (ETU), a suspected carcinogen.1.1 This test method covers the standard materials, test formulas, mixing procedures and test methods for evaluation and quality control testing of chloroprene rubbers (CR). CR can be generally classified according to the two types of polymerization modifiers used in their manufacture: sulfur modified types and mercaptan modified types.1.2 The values stated in SI-units are to be regarded as the standard. The values in parentheses are for information only.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 The test results provide an indication of the motor life of an electric motorized nozzle. End of motor life will be judged in accordance with Section 3.1.1 This test method covers the motorized nozzle used with household or commercial vacuum cleaner systems, that is, combination cleaners.1.2 This test method provides a test for determining operating motor life in hours by an accelerated laboratory procedure. The motors are tested while mounted and operated in the motorized nozzle.1.3 This test method covers only the motorized nozzle. The system used to provide the airflow source is not under consideration.1.4 This test method is limited to the determination of motor life for an electrically powered household or commercial motorized nozzle.1.5 The values stated in inch-pound units are to be regarded as the standard. The values given in parentheses are for information only.1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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This practice details the standard procedure for evaluating the resistance of prestressed prepainted metal panels to cracking, or loss of adhesion, or both, after accelerated heat aging by dry heat test. This test method requires the use of gravity or forced air laboratory oven, bench vise, bending dies or test shims, 10x magnifier, and adhesive tape.1.1 This practice can be used to evaluate the resistance of a prestressed prepainted metal panel to cracking, or loss of adhesion, or both, after accelerated heat aging. Most coil coated products are formed and bent into specific shapes to produce a final product. These operations introduce stresses, which may be relieved by cracking of the coating after aging.1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 The determination of dimensional change of pile yarn floor covering is useful in quality and cost control during manufacture of pile yarn floor covering. The appearance and performance may be affected by the changes in the dimensions of pile yarn floor covering. This test method is considered satisfactory for acceptance testing of commercial shipment because current estimates of between laboratory precision are acceptable, and this method is commonly used in the trade for acceptance testing.5.2 If there are differences of practical significance between reported test results for two laboratories (or more), comparative tests should be performed to determine if there is a statistical bias between them using competent statistical assistance. As a minimum use the samples for such comparative tests that are as homogenous as possible, drawn from the same lot of material as the samples that resulted in disparate results during initial testing and randomly assigned in equal numbers to each laboratory. The test results from the laboratories involved should be compared using a statistical test for unpaired data, a probability level chosen prior to the testing series. If a bias is found either its cause must be found and corrected, or future test results for that material must be adjusted in consideration of the known bias.1.1 This test method covers the determination of dimensional changes in the lengthwise and width-wise direction and distortion likely to occur when pile floor coverings are exposed to various conditions of moisture and heat. This method is applicable to all pile floor coverings including carpet module form.1.2 This method is applicable to machine made pile yarn floor covering.1.3 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversion to SI units that are provided for information only and are not considered standard.1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 The test results provide an indication of the motor life of an electric upright vacuum cleaner. End of motor life will be judged in accordance with Section 3.1.1 This test method covers motor life evaluation of household or commercial single and dual motor upright vacuum cleaners (uprights with separate motors for creating the vacuum and driving the floor brush). Self-propelled or power-assist cleaners are not within the scope of this test method.1.2 This test method is limited to evaluation of the upright vacuum cleaner electric motor(s).1.3 This test method provides a test to determine operating life of the motor(s), before servicing is needed, by an accelerated laboratory procedure. Motor(s) are tested while mounted and operated in the upright vacuum cleaner.1.4 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units, which are provided for information only and are not considered standard.1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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3.1 The major portion of carbon black consumed by the rubber industry is used to improve the physical properties, life expectancy, and utility of rubber products. These test methods provide an SBR recipe and directions for evaluating all types of carbon black intended for use in rubber products. Other procedures are available elsewhere in the ASTM standards for the evaluation of carbon black itself.3.2 These test methods may be used to characterize carbon black in terms of specific properties of the standard compound. These test methods are useful for the quality assurance of carbon black production. They may also be used for the preparation of reference compounds, to confirm the day-to-day reliability of testing operations used in the rubber industry, for the evaluation of experimental compounds, and quality control of production compounds.1.1 These test methods cover the standard materials, test formula, mixing procedure, and test methods for the evaluation and production control of carbon blacks in styrene butadiene rubber (SBR).1.2 The values stated in SI units are to be regarded as the standard. The values in parentheses are for information only.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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5.1 This practice should be used to determine if an antimicrobial preserves pigment suspensions, dye solutions, pulp slurries, starch solutions, polymers, sizing agents, latex emulsions, and other aqueous-based materials used in the paper industry from bacterial spoilage.NOTE 1: Control of fungal spoilage of similar products can be evaluated by Test Method E875.NOTE 2: Slimicides for control of fungal or bacterial slime can be evaluated by Test Method E1839.1.1 This laboratory practice is used to determine the efficacy of an antimicrobial for preventing bacterial spoilage of in-process aqueous-based products used in the paper industry. For information on fungal spoilage, see Test Method E875. This practice should be performed by persons who have had basic microbiological training.1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. (See 40 CFR Part 160.)1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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4.1 This guide covers animal implantation methods and analysis of the explanted DBM-containing material to determine whether a material or substance possesses osteoinductive potential, as defined by its ability to cause bone to form in vivo at a site that would otherwise not support bone formation, that is, heterotopically in a skeletal muscle implant site. For in vitro evaluation see Test Method F2131 for in vitro assessment of rhBMP 2.4.2 The test methods described here may be suitable for defining product specifications, cGMP lot release testing, research evaluation, regulatory submission, and so forth, but a positive outcome should not be presumed to indicate that the product will be osteoinductive in a human clinical application. At present, the only direct assays to assess new bone formation are in vivo, since the property of bone conduction or induction can only be assessed in a heterotopic or orthotopic site in a living animal. When these products are implanted in an orthotopic site, osteogenic factors already present at the implantation site may contribute to and enhance bone formation in conjunction with the osteoconductive nature of the product. Thus, orthotopic implantation of products may result in bone formation by acting on existing bone-forming cells and not by causing mesenchymal stem cells to become osteochondroprogenitor cells. In contrast, when these products are implanted in a heterotopic site, no native osteogenic factors are present to contribute to or enhance bone formation. Thus, heterotopic implantation of products will only result in new bone formation by causing mesenchymal stem cells to become osteochondroprogenitor cells. In vitro assays have been described and some believe they may correlate to the results obtained from in vivo assays. However such in vitro assays measure only some of the biochemical marker(s) associated with in vivo bone formation and are therefore only indirect assays for osteoinductive activity or the capacity to promote new bone formation. Many factors or combinations of factors contribute to osteoblast progenitor cells differentiating and/or proliferating into bone-forming cells in vitro that are both osteoinductive and osteoconductive when they are implanted in vivo. Thus, only an in vivo assay method currently directly considers the many potential factors involved in new bone formation induced by DBM-containing biomaterials. The qualification of a DBM or DBM-containing material should also encompass product characterization such as that described in Appendix X1.1.1 This guide covers general guidelines to evaluate the effectiveness of DBM-containing products intended to cause and/or promote bone formation when implanted or injected in vivo. This guide is applicable to products that may be composed of one or more of the following components: natural biomaterials (such as demineralized bone), and synthetic biomaterials (such as calcium sulfate, glycerol, and reverse phase polymeric compounds) that act as additives, fillers, and/or excipients (radioprotective agents, preservatives, and/or handling agents) to make the demineralized bone easier to manipulate. It should not be assumed that products evaluated favorably using this guidance will form bone when used in a clinical setting. The primary purpose of this guide is to facilitate the equitable comparison of unique bone-forming products in in vivo heterotopic models of osteoinductivity. The purpose of this guide is not to exclude other established methods.1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.1.3 This standard does not purport to address all of the safety concerns, if any, associated with the use of DBM-containing bone-forming/promoting products. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices involved in the development of said products in accordance with applicable regulatory guidance documents and in implementing this guide to evaluate the bone-forming/promoting capabilities of the product.1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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